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The Full Globin Repertoire of Turtles Provides Insights into Vertebrate Globin Evolution and Functions.

Schwarze K, Singh A, Burmester T - Genome Biol Evol (2015)

Bottom Line: Quantitative real-time reverse transcription polymerase chain reaction experiments revealed that myoglobin, neuroglobin, and globin E are highly expressed with tissue-specific patterns, which are in line with their roles in the oxidative metabolism of the striated muscles, the brain, and the retina, respectively.Histochemical analyses showed high levels of globin E in the pigment epithelium of the eye.Globin E probably has a myoglobin-like role in transporting O2 across the pigment epithelium to supply in the metabolically highly active retina.

View Article: PubMed Central - PubMed

Affiliation: Institute of Zoology, Department of Biology, University of Hamburg, Germany.

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Alignment of globins of the Chinese softshell turtle (Pelodiscus sinensis; Psi) and the western painted turtle (Chrysemys picta bellii; Cpi). The secondary structure of human Mb is superimposed in the upper row, with α-helices designated A through H, the globin consensus numbering is given below the sequences. Conserved residues are shaded (100% conservation, black; 75%, dark gray). The conserved histidine and phenylalanine residues required for oxygen binding are marked. Note that PsiHbAa harbors a glutamine instead of the histidine at helix position E7.
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evv114-F1: Alignment of globins of the Chinese softshell turtle (Pelodiscus sinensis; Psi) and the western painted turtle (Chrysemys picta bellii; Cpi). The secondary structure of human Mb is superimposed in the upper row, with α-helices designated A through H, the globin consensus numbering is given below the sequences. Conserved residues are shaded (100% conservation, black; 75%, dark gray). The conserved histidine and phenylalanine residues required for oxygen binding are marked. Note that PsiHbAa harbors a glutamine instead of the histidine at helix position E7.

Mentions: The globin gene sequences of the Chinese softshell turtle (P. sinensis) and the western painted turtle (C. picta bellii) were identified in the published genome assemblies (Shaffer et al. 2013; Wang et al. 2013) and with RACE if required. The globin repertoire of the Chinese softshell turtle comprised the full set of vertebrate globins with three Hbα and two Hbβ genes, and one of each Mb, Ngb, GbE, GbY, GbX, and Adgb gene (fig. 1 and supplementary table S2, Supplementary Material online). The western painted turtle has a higher quality in genome assembly and annotation. It harbors the same gene set and has an additional Hbβ gene (figs. 1 and 2B and supplementary table S3, Supplementary Material online). Supplementary tables S2 and S3, Supplementary Material online, summarize the names, the lengths, the accession numbers, and the genomic positions of the globins of the softshell turtle and the painted turtle, respectively.Fig. 1.—


The Full Globin Repertoire of Turtles Provides Insights into Vertebrate Globin Evolution and Functions.

Schwarze K, Singh A, Burmester T - Genome Biol Evol (2015)

Alignment of globins of the Chinese softshell turtle (Pelodiscus sinensis; Psi) and the western painted turtle (Chrysemys picta bellii; Cpi). The secondary structure of human Mb is superimposed in the upper row, with α-helices designated A through H, the globin consensus numbering is given below the sequences. Conserved residues are shaded (100% conservation, black; 75%, dark gray). The conserved histidine and phenylalanine residues required for oxygen binding are marked. Note that PsiHbAa harbors a glutamine instead of the histidine at helix position E7.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524481&req=5

evv114-F1: Alignment of globins of the Chinese softshell turtle (Pelodiscus sinensis; Psi) and the western painted turtle (Chrysemys picta bellii; Cpi). The secondary structure of human Mb is superimposed in the upper row, with α-helices designated A through H, the globin consensus numbering is given below the sequences. Conserved residues are shaded (100% conservation, black; 75%, dark gray). The conserved histidine and phenylalanine residues required for oxygen binding are marked. Note that PsiHbAa harbors a glutamine instead of the histidine at helix position E7.
Mentions: The globin gene sequences of the Chinese softshell turtle (P. sinensis) and the western painted turtle (C. picta bellii) were identified in the published genome assemblies (Shaffer et al. 2013; Wang et al. 2013) and with RACE if required. The globin repertoire of the Chinese softshell turtle comprised the full set of vertebrate globins with three Hbα and two Hbβ genes, and one of each Mb, Ngb, GbE, GbY, GbX, and Adgb gene (fig. 1 and supplementary table S2, Supplementary Material online). The western painted turtle has a higher quality in genome assembly and annotation. It harbors the same gene set and has an additional Hbβ gene (figs. 1 and 2B and supplementary table S3, Supplementary Material online). Supplementary tables S2 and S3, Supplementary Material online, summarize the names, the lengths, the accession numbers, and the genomic positions of the globins of the softshell turtle and the painted turtle, respectively.Fig. 1.—

Bottom Line: Quantitative real-time reverse transcription polymerase chain reaction experiments revealed that myoglobin, neuroglobin, and globin E are highly expressed with tissue-specific patterns, which are in line with their roles in the oxidative metabolism of the striated muscles, the brain, and the retina, respectively.Histochemical analyses showed high levels of globin E in the pigment epithelium of the eye.Globin E probably has a myoglobin-like role in transporting O2 across the pigment epithelium to supply in the metabolically highly active retina.

View Article: PubMed Central - PubMed

Affiliation: Institute of Zoology, Department of Biology, University of Hamburg, Germany.

Show MeSH