The Full Globin Repertoire of Turtles Provides Insights into Vertebrate Globin Evolution and Functions.
Bottom Line: Quantitative real-time reverse transcription polymerase chain reaction experiments revealed that myoglobin, neuroglobin, and globin E are highly expressed with tissue-specific patterns, which are in line with their roles in the oxidative metabolism of the striated muscles, the brain, and the retina, respectively.Histochemical analyses showed high levels of globin E in the pigment epithelium of the eye.Globin E probably has a myoglobin-like role in transporting O2 across the pigment epithelium to supply in the metabolically highly active retina.
Affiliation: Institute of Zoology, Department of Biology, University of Hamburg, Germany.Show MeSH
Mentions: The globin gene sequences of the Chinese softshell turtle (P. sinensis) and the western painted turtle (C. picta bellii) were identified in the published genome assemblies (Shaffer et al. 2013; Wang et al. 2013) and with RACE if required. The globin repertoire of the Chinese softshell turtle comprised the full set of vertebrate globins with three Hbα and two Hbβ genes, and one of each Mb, Ngb, GbE, GbY, GbX, and Adgb gene (fig. 1 and supplementary table S2, Supplementary Material online). The western painted turtle has a higher quality in genome assembly and annotation. It harbors the same gene set and has an additional Hbβ gene (figs. 1 and 2B and supplementary table S3, Supplementary Material online). Supplementary tables S2 and S3, Supplementary Material online, summarize the names, the lengths, the accession numbers, and the genomic positions of the globins of the softshell turtle and the painted turtle, respectively.Fig. 1.—
Affiliation: Institute of Zoology, Department of Biology, University of Hamburg, Germany.