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Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts.

Fang R, Qiao S, Liu Y, Meng Q, Chen X, Song B, Hou X, Tian W - Int J Nanomedicine (2015)

Bottom Line: However, their delivery for sustained release in MI-affected areas has proved to be challenging.The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function.Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

View Article: PubMed Central - PubMed

Affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China ; Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.

ABSTRACT
Dedifferentiation and proliferation of endogenous cardiomyocytes in situ can effectively improve cardiac repair following myocardial infarction (MI). 6-Bromoindirubin-3-oxime (BIO) and insulin-like growth factor 1 (IGF-1) are two potent factors that promote cardiomyocyte survival and proliferation. However, their delivery for sustained release in MI-affected areas has proved to be challenging. In the current research, we present a study on the sustained co-delivery of BIO and IGF-1 in a hybrid hydrogel system to simulate endogenous cardiac repair in an MI rat model. Both BIO and IGF-1 were efficiently encapsulated in gelatin nanoparticles, which were later cross-linked with the oxidized alginate to form a novel hybrid hydrogel system. The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function. Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

No MeSH data available.


Related in: MedlinePlus

Release assay profile in vitro.Notes: (A) SEM morphology of the prepared gelatin NPs. (B) DLS analysis of the gelatin NPs. (C) SEM image and a zoomed image showing the gelatin NPs covalently conjugated to the hydrogel. (D) The UV results of BIO absorption showing the release profiles in the free NP group and in the gelatin NP encapsulated in hydrogel group. (E) Mechanical properties of the hydrogel before and after release.Abbreviations: SEM, scanning electron microscope; DLS, dynamic light scattering; BIO, 6-bromoindirubin-3-oxime; NP, nanoparticle; UV, ultraviolet.
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f1-ijn-10-4691: Release assay profile in vitro.Notes: (A) SEM morphology of the prepared gelatin NPs. (B) DLS analysis of the gelatin NPs. (C) SEM image and a zoomed image showing the gelatin NPs covalently conjugated to the hydrogel. (D) The UV results of BIO absorption showing the release profiles in the free NP group and in the gelatin NP encapsulated in hydrogel group. (E) Mechanical properties of the hydrogel before and after release.Abbreviations: SEM, scanning electron microscope; DLS, dynamic light scattering; BIO, 6-bromoindirubin-3-oxime; NP, nanoparticle; UV, ultraviolet.

Mentions: The morphology of BgNPs was observed using a scanning electron microscope (SEM) (Figure 1A); the results of dynamic light scattering showed that the NPs formed a cluster featuring different diameters ranging from 180 nm to 255 nm with a polydispersity index (PDI) of 0.158±0.029 (Figure 1B). Then, the NPs were covalently attached to the hydrogel, as we have previously reported.24 The hydrogel formed with the porous network structure (Figure 1C). The loading efficiency of OgNPs and FgNPs, which contained BIO and IGF-1, respectively, were about 55% or 60%, which is similar to the findings of a previous study.28 To detect the release profile of the NPs and the hydrogel composite system, we designed a method to examine the BIO release profile in the NPs conjugated to the hydrogel and in the free NPs, as described in the encapsulation efficiencies of NPs section. We compared the release behavior of BIO in NPs and in NPs covalently conjugated to the hydrogel.


Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts.

Fang R, Qiao S, Liu Y, Meng Q, Chen X, Song B, Hou X, Tian W - Int J Nanomedicine (2015)

Release assay profile in vitro.Notes: (A) SEM morphology of the prepared gelatin NPs. (B) DLS analysis of the gelatin NPs. (C) SEM image and a zoomed image showing the gelatin NPs covalently conjugated to the hydrogel. (D) The UV results of BIO absorption showing the release profiles in the free NP group and in the gelatin NP encapsulated in hydrogel group. (E) Mechanical properties of the hydrogel before and after release.Abbreviations: SEM, scanning electron microscope; DLS, dynamic light scattering; BIO, 6-bromoindirubin-3-oxime; NP, nanoparticle; UV, ultraviolet.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524466&req=5

f1-ijn-10-4691: Release assay profile in vitro.Notes: (A) SEM morphology of the prepared gelatin NPs. (B) DLS analysis of the gelatin NPs. (C) SEM image and a zoomed image showing the gelatin NPs covalently conjugated to the hydrogel. (D) The UV results of BIO absorption showing the release profiles in the free NP group and in the gelatin NP encapsulated in hydrogel group. (E) Mechanical properties of the hydrogel before and after release.Abbreviations: SEM, scanning electron microscope; DLS, dynamic light scattering; BIO, 6-bromoindirubin-3-oxime; NP, nanoparticle; UV, ultraviolet.
Mentions: The morphology of BgNPs was observed using a scanning electron microscope (SEM) (Figure 1A); the results of dynamic light scattering showed that the NPs formed a cluster featuring different diameters ranging from 180 nm to 255 nm with a polydispersity index (PDI) of 0.158±0.029 (Figure 1B). Then, the NPs were covalently attached to the hydrogel, as we have previously reported.24 The hydrogel formed with the porous network structure (Figure 1C). The loading efficiency of OgNPs and FgNPs, which contained BIO and IGF-1, respectively, were about 55% or 60%, which is similar to the findings of a previous study.28 To detect the release profile of the NPs and the hydrogel composite system, we designed a method to examine the BIO release profile in the NPs conjugated to the hydrogel and in the free NPs, as described in the encapsulation efficiencies of NPs section. We compared the release behavior of BIO in NPs and in NPs covalently conjugated to the hydrogel.

Bottom Line: However, their delivery for sustained release in MI-affected areas has proved to be challenging.The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function.Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

View Article: PubMed Central - PubMed

Affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, People's Republic of China ; Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.

ABSTRACT
Dedifferentiation and proliferation of endogenous cardiomyocytes in situ can effectively improve cardiac repair following myocardial infarction (MI). 6-Bromoindirubin-3-oxime (BIO) and insulin-like growth factor 1 (IGF-1) are two potent factors that promote cardiomyocyte survival and proliferation. However, their delivery for sustained release in MI-affected areas has proved to be challenging. In the current research, we present a study on the sustained co-delivery of BIO and IGF-1 in a hybrid hydrogel system to simulate endogenous cardiac repair in an MI rat model. Both BIO and IGF-1 were efficiently encapsulated in gelatin nanoparticles, which were later cross-linked with the oxidized alginate to form a novel hybrid hydrogel system. The in vivo results indicated that the hybrid system could enhance the proliferation of cardiomyocytes in situ and could promote revascularization around the MI sites, allowing improved cardiac function. Taken together, we concluded that the hybrid hydrogel system can co-deliver BIO and IGF-1 to areas of MI and thus improve cardiac function by promoting the proliferation of cardiomyocytes and revascularization.

No MeSH data available.


Related in: MedlinePlus