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Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus

Relative viabilities of MDA-MB-231 cells after being incubated with (A) PMSG and TMSG nanoparticles or (B) I-PMSG and I-TMSG nanoparticles for 48 hours (n=4).Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; I-PMSG, indocyanine green-containing PMSG; I-TMSG, indocyanine green-containing TMSG; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
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f9-ijn-10-4747: Relative viabilities of MDA-MB-231 cells after being incubated with (A) PMSG and TMSG nanoparticles or (B) I-PMSG and I-TMSG nanoparticles for 48 hours (n=4).Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; I-PMSG, indocyanine green-containing PMSG; I-TMSG, indocyanine green-containing TMSG; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.

Mentions: Before conducting PTT experiments, we first assessed in vitro dark cytotoxicity of TMSG and PMSG by the cell viability assay. As consistent with the previous report, silicon or gold nanomaterials in itself show negligible cytotoxicity. We found that TMSG and PMSG exhibited low cytotoxicity (cell survival rate exceeded 90%) even at nanoparticle concentrations as high as 170 μg/mL (Figure 9A). ICG-containing nanoparticles also show good cellular compatibility under dark, and the cell viability could exceed 80% at a concentration of 120 μg/mL (Figure 9B). This result also indicated that ICG, as a FDA-approved clinical imaging agent, presented low dark cytotoxicity.


Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Relative viabilities of MDA-MB-231 cells after being incubated with (A) PMSG and TMSG nanoparticles or (B) I-PMSG and I-TMSG nanoparticles for 48 hours (n=4).Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; I-PMSG, indocyanine green-containing PMSG; I-TMSG, indocyanine green-containing TMSG; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524460&req=5

f9-ijn-10-4747: Relative viabilities of MDA-MB-231 cells after being incubated with (A) PMSG and TMSG nanoparticles or (B) I-PMSG and I-TMSG nanoparticles for 48 hours (n=4).Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; I-PMSG, indocyanine green-containing PMSG; I-TMSG, indocyanine green-containing TMSG; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
Mentions: Before conducting PTT experiments, we first assessed in vitro dark cytotoxicity of TMSG and PMSG by the cell viability assay. As consistent with the previous report, silicon or gold nanomaterials in itself show negligible cytotoxicity. We found that TMSG and PMSG exhibited low cytotoxicity (cell survival rate exceeded 90%) even at nanoparticle concentrations as high as 170 μg/mL (Figure 9A). ICG-containing nanoparticles also show good cellular compatibility under dark, and the cell viability could exceed 80% at a concentration of 120 μg/mL (Figure 9B). This result also indicated that ICG, as a FDA-approved clinical imaging agent, presented low dark cytotoxicity.

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus