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Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus

Transmission electron micrograph images of (A) GNRs (inset: the photo of CTAB-stabilized GNRs solution) and (B) MSG. (C) High-magnification transmission electron micrograph image of MSG. (D) UV–vis spectra of GNRs and MSG. (E) N2 adsorption–desorption isotherms. (F) The pore diameter distribution of MSG.Abbreviations: GNRs, gold nanorods; CTAB, cetyltrimethylammonium bromide; MSG, mesoporous silica-coated GNRs; UV, ultraviolet; SPT, standard pressure and temperature.
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f3-ijn-10-4747: Transmission electron micrograph images of (A) GNRs (inset: the photo of CTAB-stabilized GNRs solution) and (B) MSG. (C) High-magnification transmission electron micrograph image of MSG. (D) UV–vis spectra of GNRs and MSG. (E) N2 adsorption–desorption isotherms. (F) The pore diameter distribution of MSG.Abbreviations: GNRs, gold nanorods; CTAB, cetyltrimethylammonium bromide; MSG, mesoporous silica-coated GNRs; UV, ultraviolet; SPT, standard pressure and temperature.

Mentions: By using well-known seed-mediation growth method and careful control of pH, GNRs of relatively high aspect ratio (3.50±0.37, n=25) have been produced by surfactant-directed synthesis. Then, by referring to Huang’s reports48 with slight modifications, a 17.34–32.43-nm thick mesoporous silica layer wrapped around GNRs was formed via sol–gel reaction based on the hydrolysis of TEOS. By atomic absorption spectroscopy assay, the Au contents in MSG nanoparticles were 42.92%±1.32% (n=3). The GNRs, as shown in the TEM images (Figure 3A), were rodlike, and the width and length were 47.23±4.92 nm and 13.56±1.48 nm (n=25), respectively. However, the MSG nanoparticles were generally spherical in shape with good monodispersity (Figures 3B and 4A). The widely distributed mesoporous of MSG, which acted as drug or imaging-agent cargo-space, could even be observed from the high-resolution TEM images (Figure 3C).


Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Transmission electron micrograph images of (A) GNRs (inset: the photo of CTAB-stabilized GNRs solution) and (B) MSG. (C) High-magnification transmission electron micrograph image of MSG. (D) UV–vis spectra of GNRs and MSG. (E) N2 adsorption–desorption isotherms. (F) The pore diameter distribution of MSG.Abbreviations: GNRs, gold nanorods; CTAB, cetyltrimethylammonium bromide; MSG, mesoporous silica-coated GNRs; UV, ultraviolet; SPT, standard pressure and temperature.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524460&req=5

f3-ijn-10-4747: Transmission electron micrograph images of (A) GNRs (inset: the photo of CTAB-stabilized GNRs solution) and (B) MSG. (C) High-magnification transmission electron micrograph image of MSG. (D) UV–vis spectra of GNRs and MSG. (E) N2 adsorption–desorption isotherms. (F) The pore diameter distribution of MSG.Abbreviations: GNRs, gold nanorods; CTAB, cetyltrimethylammonium bromide; MSG, mesoporous silica-coated GNRs; UV, ultraviolet; SPT, standard pressure and temperature.
Mentions: By using well-known seed-mediation growth method and careful control of pH, GNRs of relatively high aspect ratio (3.50±0.37, n=25) have been produced by surfactant-directed synthesis. Then, by referring to Huang’s reports48 with slight modifications, a 17.34–32.43-nm thick mesoporous silica layer wrapped around GNRs was formed via sol–gel reaction based on the hydrolysis of TEOS. By atomic absorption spectroscopy assay, the Au contents in MSG nanoparticles were 42.92%±1.32% (n=3). The GNRs, as shown in the TEM images (Figure 3A), were rodlike, and the width and length were 47.23±4.92 nm and 13.56±1.48 nm (n=25), respectively. However, the MSG nanoparticles were generally spherical in shape with good monodispersity (Figures 3B and 4A). The widely distributed mesoporous of MSG, which acted as drug or imaging-agent cargo-space, could even be observed from the high-resolution TEM images (Figure 3C).

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus