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Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus

A schematic procedure for the preparation of TMSG nanoparticles.Abbreviations: TMSG, tLyp-1 peptide-functionalized and polyethylene glycol-modified mesoporous silica-coated gold nanorods; APTES, aminopropyl triethoxysilane; NHS-PEG3500-MAL maleimide PEG N-hydroxylsuccinimide ester; CTAB, cetyltrimethylammonium bromide; h, hours; TEOS, tetraethyl orthosilicate.
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f2-ijn-10-4747: A schematic procedure for the preparation of TMSG nanoparticles.Abbreviations: TMSG, tLyp-1 peptide-functionalized and polyethylene glycol-modified mesoporous silica-coated gold nanorods; APTES, aminopropyl triethoxysilane; NHS-PEG3500-MAL maleimide PEG N-hydroxylsuccinimide ester; CTAB, cetyltrimethylammonium bromide; h, hours; TEOS, tetraethyl orthosilicate.

Mentions: The synthesis of GNRs, GNRs@mSiO2 (MSG), PEG-GNRs@mSiO2 (PMSG), and PEG-GNRs@mSiO2-tLyp-1 (TMSG) is schematically illustrated in Figure 2. GNRs were prepared by seed-mediated growth method with minor revisions.47 First, 600 μL of NaBH4 (0.01 M) was mixed with 9.4 mL of aqueous solution containing 7.5 mL of CTAB (0.1 M) and 250 μL of HAuCl4 (0.01 M). It was then kept at 30°C for 3 hours before being further used as seed solution. Second, the growth solution contained 100 mL of CTAB (0.1 M), 5 mL of HAuCl4 (0.01 M), 800 μL of AgNO3 (0.01 M), 2 mL of H2SO4 (0.5 M), and 800 μL of ascorbic acid (0.1 M). After vigorous stirring of the growth solution, 240 μL of seed solution was added and let to stew for another 15 hours at 30°C to obtain GNRs.


Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

A schematic procedure for the preparation of TMSG nanoparticles.Abbreviations: TMSG, tLyp-1 peptide-functionalized and polyethylene glycol-modified mesoporous silica-coated gold nanorods; APTES, aminopropyl triethoxysilane; NHS-PEG3500-MAL maleimide PEG N-hydroxylsuccinimide ester; CTAB, cetyltrimethylammonium bromide; h, hours; TEOS, tetraethyl orthosilicate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524460&req=5

f2-ijn-10-4747: A schematic procedure for the preparation of TMSG nanoparticles.Abbreviations: TMSG, tLyp-1 peptide-functionalized and polyethylene glycol-modified mesoporous silica-coated gold nanorods; APTES, aminopropyl triethoxysilane; NHS-PEG3500-MAL maleimide PEG N-hydroxylsuccinimide ester; CTAB, cetyltrimethylammonium bromide; h, hours; TEOS, tetraethyl orthosilicate.
Mentions: The synthesis of GNRs, GNRs@mSiO2 (MSG), PEG-GNRs@mSiO2 (PMSG), and PEG-GNRs@mSiO2-tLyp-1 (TMSG) is schematically illustrated in Figure 2. GNRs were prepared by seed-mediated growth method with minor revisions.47 First, 600 μL of NaBH4 (0.01 M) was mixed with 9.4 mL of aqueous solution containing 7.5 mL of CTAB (0.1 M) and 250 μL of HAuCl4 (0.01 M). It was then kept at 30°C for 3 hours before being further used as seed solution. Second, the growth solution contained 100 mL of CTAB (0.1 M), 5 mL of HAuCl4 (0.01 M), 800 μL of AgNO3 (0.01 M), 2 mL of H2SO4 (0.5 M), and 800 μL of ascorbic acid (0.1 M). After vigorous stirring of the growth solution, 240 μL of seed solution was added and let to stew for another 15 hours at 30°C to obtain GNRs.

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus