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Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus

(A) Relative viabilities of MDA-MB-231 cells incubated with TMSG or PMSG at different concentrations under exposure to a 785 nm laser at a power density of 3 W/cm2 for 5 minutes. (B) Relative viabilities of MDA-MB-231 cells after TMSG (80 μg/mL) induced photothermal ablation over different laser exposure periods.Notes: The cell viability values were all normalized to control untreated cells (n=3). **P<0.01.Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; s, seconds; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
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f13-ijn-10-4747: (A) Relative viabilities of MDA-MB-231 cells incubated with TMSG or PMSG at different concentrations under exposure to a 785 nm laser at a power density of 3 W/cm2 for 5 minutes. (B) Relative viabilities of MDA-MB-231 cells after TMSG (80 μg/mL) induced photothermal ablation over different laser exposure periods.Notes: The cell viability values were all normalized to control untreated cells (n=3). **P<0.01.Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; s, seconds; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.

Mentions: Finally, cell viability test based on CCK-8 assay was performed. Under a 785-nm laser exposure at a density of 3 W/cm2 for 5 minutes, enhanced photothermal ablation efficacy was observed as the increase in nanoparticle concentrations. More importantly, TMSG showed a significantly stronger cell-killing effect compared with PMSG under the same nanoparticle concentrations (P<0.01, Figure 13A), which might be attributed to its robust endocytosis. Besides, PTT effects could be enhanced by increasing laser irradiation time from 30 seconds to 3 minutes. However, relative cell viability was <20% by using a dose of 80 μg/mL of TMSG plus 3 minutes NIR irradiation with power density of 3 W/cm2 (Figure 13B). All together, these data strongly confirmed that the enhanced photothermal toxicity of tLyp-1 modified TMSG compared with PMSG and further suggested that TMSG nanoparticles could be an effective and promising active-targeting PTT agent.


Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

(A) Relative viabilities of MDA-MB-231 cells incubated with TMSG or PMSG at different concentrations under exposure to a 785 nm laser at a power density of 3 W/cm2 for 5 minutes. (B) Relative viabilities of MDA-MB-231 cells after TMSG (80 μg/mL) induced photothermal ablation over different laser exposure periods.Notes: The cell viability values were all normalized to control untreated cells (n=3). **P<0.01.Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; s, seconds; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524460&req=5

f13-ijn-10-4747: (A) Relative viabilities of MDA-MB-231 cells incubated with TMSG or PMSG at different concentrations under exposure to a 785 nm laser at a power density of 3 W/cm2 for 5 minutes. (B) Relative viabilities of MDA-MB-231 cells after TMSG (80 μg/mL) induced photothermal ablation over different laser exposure periods.Notes: The cell viability values were all normalized to control untreated cells (n=3). **P<0.01.Abbreviations: PMSG, polyethylene glycol-modified mesoporous silica-coated gold nanorods; TMSG, tLyp-1 peptide-functionalized PMSG; s, seconds; MDA-MB-231cells, MD Anderson-metastatic breast-231 cells.
Mentions: Finally, cell viability test based on CCK-8 assay was performed. Under a 785-nm laser exposure at a density of 3 W/cm2 for 5 minutes, enhanced photothermal ablation efficacy was observed as the increase in nanoparticle concentrations. More importantly, TMSG showed a significantly stronger cell-killing effect compared with PMSG under the same nanoparticle concentrations (P<0.01, Figure 13A), which might be attributed to its robust endocytosis. Besides, PTT effects could be enhanced by increasing laser irradiation time from 30 seconds to 3 minutes. However, relative cell viability was <20% by using a dose of 80 μg/mL of TMSG plus 3 minutes NIR irradiation with power density of 3 W/cm2 (Figure 13B). All together, these data strongly confirmed that the enhanced photothermal toxicity of tLyp-1 modified TMSG compared with PMSG and further suggested that TMSG nanoparticles could be an effective and promising active-targeting PTT agent.

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus