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Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry analysis of MDA-MB-231 cells after photothermal therapy treatments under different treatment conditions.Notes: The living cell fraction is negative for both Annexin V-FITC and propidium iodide. An earlier stage of apoptosis is linked with positive Annexin V-FITC labeling only. Double-stained cells were considered as necrotic/late apoptotic cells. The concentration of TMSG was 70 μg/mL, and the power density of laser irradiation was 3 W/cm2.Abbreviations: TMSG, tLyp-1 peptide and polyethylene glycol-comodified mesoporous silica-coated gold nanorods; PI, propidium iodide; FL, fluorescence; MDA-MB-231 cells, MD Anderson-metastatic breast-231 cells; min, minutes.
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f12-ijn-10-4747: Flow cytometry analysis of MDA-MB-231 cells after photothermal therapy treatments under different treatment conditions.Notes: The living cell fraction is negative for both Annexin V-FITC and propidium iodide. An earlier stage of apoptosis is linked with positive Annexin V-FITC labeling only. Double-stained cells were considered as necrotic/late apoptotic cells. The concentration of TMSG was 70 μg/mL, and the power density of laser irradiation was 3 W/cm2.Abbreviations: TMSG, tLyp-1 peptide and polyethylene glycol-comodified mesoporous silica-coated gold nanorods; PI, propidium iodide; FL, fluorescence; MDA-MB-231 cells, MD Anderson-metastatic breast-231 cells; min, minutes.

Mentions: The PTT effects were further confirmed with apoptosis detection by flow cytometry. The tumor cells were treated with PTT and then double-labeled with Annexin V-FITC and PI successively. The results show that PTT treatment significantly induced tumor cell necrosis/late apoptosis (72.9%) compared with control groups (12.7%, Figure 12).


Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser.

Liu Y, Xu M, Chen Q, Guan G, Hu W, Zhao X, Qiao M, Hu H, Liang Y, Zhu H, Chen D - Int J Nanomedicine (2015)

Flow cytometry analysis of MDA-MB-231 cells after photothermal therapy treatments under different treatment conditions.Notes: The living cell fraction is negative for both Annexin V-FITC and propidium iodide. An earlier stage of apoptosis is linked with positive Annexin V-FITC labeling only. Double-stained cells were considered as necrotic/late apoptotic cells. The concentration of TMSG was 70 μg/mL, and the power density of laser irradiation was 3 W/cm2.Abbreviations: TMSG, tLyp-1 peptide and polyethylene glycol-comodified mesoporous silica-coated gold nanorods; PI, propidium iodide; FL, fluorescence; MDA-MB-231 cells, MD Anderson-metastatic breast-231 cells; min, minutes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524460&req=5

f12-ijn-10-4747: Flow cytometry analysis of MDA-MB-231 cells after photothermal therapy treatments under different treatment conditions.Notes: The living cell fraction is negative for both Annexin V-FITC and propidium iodide. An earlier stage of apoptosis is linked with positive Annexin V-FITC labeling only. Double-stained cells were considered as necrotic/late apoptotic cells. The concentration of TMSG was 70 μg/mL, and the power density of laser irradiation was 3 W/cm2.Abbreviations: TMSG, tLyp-1 peptide and polyethylene glycol-comodified mesoporous silica-coated gold nanorods; PI, propidium iodide; FL, fluorescence; MDA-MB-231 cells, MD Anderson-metastatic breast-231 cells; min, minutes.
Mentions: The PTT effects were further confirmed with apoptosis detection by flow cytometry. The tumor cells were treated with PTT and then double-labeled with Annexin V-FITC and PI successively. The results show that PTT treatment significantly induced tumor cell necrosis/late apoptosis (72.9%) compared with control groups (12.7%, Figure 12).

Bottom Line: The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous.The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity.More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People's Republic of China ; Department of Pharmacy, Bengbu Medical College, Bengbu, People's Republic of China.

ABSTRACT
Photothermal therapy (PTT) is widely regarded as a promising technology for cancer treatment. Gold nanorods (GNRs), as excellent PTT agent candidates, have shown high-performance photothermal conversion ability under laser irradiation, yet two major obstacles to their clinical application are the lack of selective accumulation in the target site following systemic administration and the greatly reduced photothermal conversion efficiency caused by self-aggregating in aqueous environment. Herein, we demonstrate that tLyp-1 peptide-functionalized, indocyanine green (ICG)-containing mesoporous silica-coated GNRs (I-TMSG) possessed dual-function as tumor cells-targeting near-infrared (NIR) fluorescent probe and PTT agents. The construction of the nanostructure began with synthesis of GNRs by seed-mediated growth method, followed by the coating of mesoporous silica, the chemical conjugation of PEG and tLyp-1 peptide, and the enclosure of ICG as an NIR imaging agent in the mesoporous. The as-prepared nanoparticles could shield the GNRs against their self-aggregation, improve the stability of ICG, and exhibit negligible dark cytotoxicity. More importantly, such a theranostic nanocomposite could realize the combination of GNRs-based photothermal ablation under NIR illumination, ICG-mediated fluorescent imaging, and tLyp-1-enabled more easy endocytosis into breast cancer cells. All in all, I-TMSG nanoparticles, in our opinion, possessed the strong potential to realize the effective diagnosis and PTT treatment of human mammary cancer.

No MeSH data available.


Related in: MedlinePlus