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Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study.

Pan CH, Chuang KJ, Chen JK, Hsiao TC, Lai CH, Jones TP, BéruBé KA, Hong GB, Ho KF, Chuang HC - Int J Nanomedicine (2015)

Bottom Line: An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways.A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein.Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times.

View Article: PubMed Central - PubMed

Affiliation: Institute of Occupational Safety and Health, Council of Labor Affairs, Executive Yuan, Taiwan ; School of Public Health, National Defense Medical Center, Taipei Medical University, Taipei, Taiwan.

ABSTRACT
Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.

No MeSH data available.


Related in: MedlinePlus

SEM photographs of commercial 20 nm-diameter ZnONPs.Notes: The shapes of the ZnONPs were hexagonal columns of various lengths. Only Zn and O detected in the ZnONP with the addition of C (from the carbon stick) and Pt (from the coating material) as indicated by EDX analysis.Abbreviations: SEM, scanning electron microscopy; ZnONPs, zinc oxide nanoparticles; EDX, energy-dispersive X-ray.
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f2-ijn-10-4705: SEM photographs of commercial 20 nm-diameter ZnONPs.Notes: The shapes of the ZnONPs were hexagonal columns of various lengths. Only Zn and O detected in the ZnONP with the addition of C (from the carbon stick) and Pt (from the coating material) as indicated by EDX analysis.Abbreviations: SEM, scanning electron microscopy; ZnONPs, zinc oxide nanoparticles; EDX, energy-dispersive X-ray.

Mentions: ZnONPs with an average diameter of 20 nm were used in this study. The basic characteristics of the ZnONP as provided by the supplier were 99.5 wt% purity and 50 m2/g specific surface area. The shapes of most of the ZnONPs were hexagonal columns of various lengths (Figure 2). Only Zn and O were detected in the ZnONP, except for C (from the carbon tab) and Pt (from the coating material) (Figure 2).


Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study.

Pan CH, Chuang KJ, Chen JK, Hsiao TC, Lai CH, Jones TP, BéruBé KA, Hong GB, Ho KF, Chuang HC - Int J Nanomedicine (2015)

SEM photographs of commercial 20 nm-diameter ZnONPs.Notes: The shapes of the ZnONPs were hexagonal columns of various lengths. Only Zn and O detected in the ZnONP with the addition of C (from the carbon stick) and Pt (from the coating material) as indicated by EDX analysis.Abbreviations: SEM, scanning electron microscopy; ZnONPs, zinc oxide nanoparticles; EDX, energy-dispersive X-ray.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4524458&req=5

f2-ijn-10-4705: SEM photographs of commercial 20 nm-diameter ZnONPs.Notes: The shapes of the ZnONPs were hexagonal columns of various lengths. Only Zn and O detected in the ZnONP with the addition of C (from the carbon stick) and Pt (from the coating material) as indicated by EDX analysis.Abbreviations: SEM, scanning electron microscopy; ZnONPs, zinc oxide nanoparticles; EDX, energy-dispersive X-ray.
Mentions: ZnONPs with an average diameter of 20 nm were used in this study. The basic characteristics of the ZnONP as provided by the supplier were 99.5 wt% purity and 50 m2/g specific surface area. The shapes of most of the ZnONPs were hexagonal columns of various lengths (Figure 2). Only Zn and O were detected in the ZnONP, except for C (from the carbon tab) and Pt (from the coating material) (Figure 2).

Bottom Line: An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways.A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein.Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times.

View Article: PubMed Central - PubMed

Affiliation: Institute of Occupational Safety and Health, Council of Labor Affairs, Executive Yuan, Taiwan ; School of Public Health, National Defense Medical Center, Taipei Medical University, Taipei, Taiwan.

ABSTRACT
Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.

No MeSH data available.


Related in: MedlinePlus