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Accessible Synthetic Probes for Staining Actin inside Platelets and Megakaryocytes by Employing Lifeact Peptide.

Cardo L, Thomas SG, Mazharian A, Pikramenou Z, Rappoport JZ, Hannon MJ, Watson SP - Chembiochem (2015)

Bottom Line: Lifeact is a 17-residue peptide that can be employed in cell microscopy as a probe for F-actin when fused to fluorescent proteins, but therefore is not suitable for all cell types.Using confocal microscopy, we characterised the cell distribution of the new hybrids in fixed cells, and found that both myr- and pHLIP-Lifeact conjugates provide efficient actin staining upon cleavage of Lifeact from the carriers, without affecting cell spreading.This new approach could facilitate the design of new tools for actin visualisation.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT (UK).

No MeSH data available.


Related in: MedlinePlus

Synthesis of carrier–Lifeact hybrids. Scheme of conjugation between carriers (labelled with TAMRA) and Lifeact (labelled with FAM) by disulfide bond exchange and a list of the cleavable (by disulfide reduction) and uncleavable compounds investigated for cell imaging.
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sch01: Synthesis of carrier–Lifeact hybrids. Scheme of conjugation between carriers (labelled with TAMRA) and Lifeact (labelled with FAM) by disulfide bond exchange and a list of the cleavable (by disulfide reduction) and uncleavable compounds investigated for cell imaging.


Accessible Synthetic Probes for Staining Actin inside Platelets and Megakaryocytes by Employing Lifeact Peptide.

Cardo L, Thomas SG, Mazharian A, Pikramenou Z, Rappoport JZ, Hannon MJ, Watson SP - Chembiochem (2015)

Synthesis of carrier–Lifeact hybrids. Scheme of conjugation between carriers (labelled with TAMRA) and Lifeact (labelled with FAM) by disulfide bond exchange and a list of the cleavable (by disulfide reduction) and uncleavable compounds investigated for cell imaging.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4524417&req=5

sch01: Synthesis of carrier–Lifeact hybrids. Scheme of conjugation between carriers (labelled with TAMRA) and Lifeact (labelled with FAM) by disulfide bond exchange and a list of the cleavable (by disulfide reduction) and uncleavable compounds investigated for cell imaging.
Bottom Line: Lifeact is a 17-residue peptide that can be employed in cell microscopy as a probe for F-actin when fused to fluorescent proteins, but therefore is not suitable for all cell types.Using confocal microscopy, we characterised the cell distribution of the new hybrids in fixed cells, and found that both myr- and pHLIP-Lifeact conjugates provide efficient actin staining upon cleavage of Lifeact from the carriers, without affecting cell spreading.This new approach could facilitate the design of new tools for actin visualisation.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT (UK).

No MeSH data available.


Related in: MedlinePlus