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Secondary Data Analytics of Aquaporin Expression Levels in Glioblastoma Stem-Like Cells.

Isokpehi RD, Wollenberg Valero KC, Graham BE, Pacurari M, Sims JN, Udensi UK, Ndebele K - Cancer Inform (2015)

Bottom Line: However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples.Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker).Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Engineering, and Mathematics, Bethune-Cookman University, Daytona Beach, FL, USA.

ABSTRACT
Glioblastoma is the most common brain tumor in adults in which recurrence has been attributed to the presence of cancer stem cells in a hypoxic microenvironment. On the basis of tumor formation in vivo and growth type in vitro, two published microarray gene expression profiling studies grouped nine glioblastoma stem-like (GS) cell lines into one of two groups: full (GSf) or restricted (GSr) stem-like phenotypes. Aquaporin-1 (AQP1) and aquaporin-4 (AQP4) are water transport proteins that are highly expressed in primary glial-derived tumors. However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples. Therefore, we designed secondary data analytics methods to determine the expression levels of AQP1 and AQP4 in GS cell lines and glioblastoma neurospheres. Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker). Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes. Discriminant function analysis further revealed that AQP1 and AQP4 expression are better predictors for tumor formation and growth types in glioblastoma stem-like cells than are CXCR4 and PROM1. Future investigations are needed to characterize the molecular mechanisms for inverse expression levels of AQP1 and AQP4 in the glioblastoma stem-like neurospheres.

No MeSH data available.


Related in: MedlinePlus

Predictive inverse expression levels of AQP1 and AQP4 in glioblastoma stem-like cells.
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Related In: Results  -  Collection


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f5-cin-14-2015-095: Predictive inverse expression levels of AQP1 and AQP4 in glioblastoma stem-like cells.

Mentions: Figure 5 aligns the AQP expression in growth types and tumor formation. For AQP1 and AQP4, Kruskal–Wallis ANOVA retrieved highly significant differences between all categories in both tests as well as differences between AQP1 and AQP4 expression in tumor formation and growth (Table 1). Grouping expression patterns according to group mean showed that AQP1 expression in spherical growth cultures, diffuse tumors, and solid tumors was lower than in semi-adherent and adherent growth cultures, as well as in instances where no tumor was formed. Further, AQP4 expression was lower in semi-adherent and adherent cultures as well as in solid and no tumors than in diffuse tumors and spherical growth cultures.


Secondary Data Analytics of Aquaporin Expression Levels in Glioblastoma Stem-Like Cells.

Isokpehi RD, Wollenberg Valero KC, Graham BE, Pacurari M, Sims JN, Udensi UK, Ndebele K - Cancer Inform (2015)

Predictive inverse expression levels of AQP1 and AQP4 in glioblastoma stem-like cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4524166&req=5

f5-cin-14-2015-095: Predictive inverse expression levels of AQP1 and AQP4 in glioblastoma stem-like cells.
Mentions: Figure 5 aligns the AQP expression in growth types and tumor formation. For AQP1 and AQP4, Kruskal–Wallis ANOVA retrieved highly significant differences between all categories in both tests as well as differences between AQP1 and AQP4 expression in tumor formation and growth (Table 1). Grouping expression patterns according to group mean showed that AQP1 expression in spherical growth cultures, diffuse tumors, and solid tumors was lower than in semi-adherent and adherent growth cultures, as well as in instances where no tumor was formed. Further, AQP4 expression was lower in semi-adherent and adherent cultures as well as in solid and no tumors than in diffuse tumors and spherical growth cultures.

Bottom Line: However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples.Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker).Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Engineering, and Mathematics, Bethune-Cookman University, Daytona Beach, FL, USA.

ABSTRACT
Glioblastoma is the most common brain tumor in adults in which recurrence has been attributed to the presence of cancer stem cells in a hypoxic microenvironment. On the basis of tumor formation in vivo and growth type in vitro, two published microarray gene expression profiling studies grouped nine glioblastoma stem-like (GS) cell lines into one of two groups: full (GSf) or restricted (GSr) stem-like phenotypes. Aquaporin-1 (AQP1) and aquaporin-4 (AQP4) are water transport proteins that are highly expressed in primary glial-derived tumors. However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples. Therefore, we designed secondary data analytics methods to determine the expression levels of AQP1 and AQP4 in GS cell lines and glioblastoma neurospheres. Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker). Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes. Discriminant function analysis further revealed that AQP1 and AQP4 expression are better predictors for tumor formation and growth types in glioblastoma stem-like cells than are CXCR4 and PROM1. Future investigations are needed to characterize the molecular mechanisms for inverse expression levels of AQP1 and AQP4 in the glioblastoma stem-like neurospheres.

No MeSH data available.


Related in: MedlinePlus