Limits...
Secondary Data Analytics of Aquaporin Expression Levels in Glioblastoma Stem-Like Cells.

Isokpehi RD, Wollenberg Valero KC, Graham BE, Pacurari M, Sims JN, Udensi UK, Ndebele K - Cancer Inform (2015)

Bottom Line: However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples.Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker).Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Engineering, and Mathematics, Bethune-Cookman University, Daytona Beach, FL, USA.

ABSTRACT
Glioblastoma is the most common brain tumor in adults in which recurrence has been attributed to the presence of cancer stem cells in a hypoxic microenvironment. On the basis of tumor formation in vivo and growth type in vitro, two published microarray gene expression profiling studies grouped nine glioblastoma stem-like (GS) cell lines into one of two groups: full (GSf) or restricted (GSr) stem-like phenotypes. Aquaporin-1 (AQP1) and aquaporin-4 (AQP4) are water transport proteins that are highly expressed in primary glial-derived tumors. However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples. Therefore, we designed secondary data analytics methods to determine the expression levels of AQP1 and AQP4 in GS cell lines and glioblastoma neurospheres. Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker). Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes. Discriminant function analysis further revealed that AQP1 and AQP4 expression are better predictors for tumor formation and growth types in glioblastoma stem-like cells than are CXCR4 and PROM1. Future investigations are needed to characterize the molecular mechanisms for inverse expression levels of AQP1 and AQP4 in the glioblastoma stem-like neurospheres.

No MeSH data available.


Related in: MedlinePlus

Comparison of expression levels for selected probe sets of AQP1, AQP4, CXCR4, and PROM1 in glioblastoma neurospheres. GSf, full stem-like phenotype, GS-3; GSr, restricted stem-like phenotype, GS-7. Interactive version of figure is available at https://public.tableausoftware.com/views/glioma_aqp/figure4.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4524166&req=5

f4-cin-14-2015-095: Comparison of expression levels for selected probe sets of AQP1, AQP4, CXCR4, and PROM1 in glioblastoma neurospheres. GSf, full stem-like phenotype, GS-3; GSr, restricted stem-like phenotype, GS-7. Interactive version of figure is available at https://public.tableausoftware.com/views/glioma_aqp/figure4.

Mentions: Another set of statements evaluated using visual representations (Fig. 4) was: “In conclusion, the group of GSf cell lines emerges as a more representative model for human glioblastomas than other GS lines or conventional glioma cell lines, mirroring original tumor gene expression signatures most closely and maintaining highly invasive growth in vivo as well as stem cell characteristics in vitro.”6


Secondary Data Analytics of Aquaporin Expression Levels in Glioblastoma Stem-Like Cells.

Isokpehi RD, Wollenberg Valero KC, Graham BE, Pacurari M, Sims JN, Udensi UK, Ndebele K - Cancer Inform (2015)

Comparison of expression levels for selected probe sets of AQP1, AQP4, CXCR4, and PROM1 in glioblastoma neurospheres. GSf, full stem-like phenotype, GS-3; GSr, restricted stem-like phenotype, GS-7. Interactive version of figure is available at https://public.tableausoftware.com/views/glioma_aqp/figure4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4524166&req=5

f4-cin-14-2015-095: Comparison of expression levels for selected probe sets of AQP1, AQP4, CXCR4, and PROM1 in glioblastoma neurospheres. GSf, full stem-like phenotype, GS-3; GSr, restricted stem-like phenotype, GS-7. Interactive version of figure is available at https://public.tableausoftware.com/views/glioma_aqp/figure4.
Mentions: Another set of statements evaluated using visual representations (Fig. 4) was: “In conclusion, the group of GSf cell lines emerges as a more representative model for human glioblastomas than other GS lines or conventional glioma cell lines, mirroring original tumor gene expression signatures most closely and maintaining highly invasive growth in vivo as well as stem cell characteristics in vitro.”6

Bottom Line: However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples.Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker).Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes.

View Article: PubMed Central - PubMed

Affiliation: College of Science, Engineering, and Mathematics, Bethune-Cookman University, Daytona Beach, FL, USA.

ABSTRACT
Glioblastoma is the most common brain tumor in adults in which recurrence has been attributed to the presence of cancer stem cells in a hypoxic microenvironment. On the basis of tumor formation in vivo and growth type in vitro, two published microarray gene expression profiling studies grouped nine glioblastoma stem-like (GS) cell lines into one of two groups: full (GSf) or restricted (GSr) stem-like phenotypes. Aquaporin-1 (AQP1) and aquaporin-4 (AQP4) are water transport proteins that are highly expressed in primary glial-derived tumors. However, the expression levels of AQP1 and AQP4 have not been previously described in a panel of 92 glioma samples. Therefore, we designed secondary data analytics methods to determine the expression levels of AQP1 and AQP4 in GS cell lines and glioblastoma neurospheres. Our investigation also included a total of 2,566 expression levels from 28 Affymetrix microarray probe sets encoding 13 human aquaporins (AQP0-AQP12); CXCR4 (the receptor for stromal cell derived factor-1 [SDF-1], a potential glioma stem cell therapeutic target]); and PROM1 (gene encoding CD133, the widely used glioma stem cell marker). Interactive visual representation designs for integrating phenotypic features and expression levels revealed that inverse expression levels of AQP1 and AQP4 correlate with distinct phenotypes in a set of cell lines grouped into full and restricted stem-like phenotypes. Discriminant function analysis further revealed that AQP1 and AQP4 expression are better predictors for tumor formation and growth types in glioblastoma stem-like cells than are CXCR4 and PROM1. Future investigations are needed to characterize the molecular mechanisms for inverse expression levels of AQP1 and AQP4 in the glioblastoma stem-like neurospheres.

No MeSH data available.


Related in: MedlinePlus