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Angiotensin-converting enzyme inhibitors of Bothrops jararaca snake venom affect the structure of mice seminiferous epithelium.

Alberto-Silva C, Gilio JM, Portaro FC, Querobino SM, Camargo AC - J Venom Anim Toxins Incl Trop Dis (2015)

Bottom Line: In addition, both synthetic peptides led to a significant reduction in the number of spermatocytes and round spermatids in stages I, V and VII/VIII of the seminiferous cycle, thickness of the seminiferous epithelium and diameter of the seminiferous tubule lumen.Interestingly, no morphological or morphometric alterations were observed in animals treated with captopril or BPP-11e.The major finding of the present study was that the demonstrated effects of BPP-10c and BPP-AP on the seminiferous epithelium are dependent on their primary structure and cannot be extrapolated to other BPPs.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural and Humanities Sciences (CCNH), Federal University of ABC (UFABC), R. Santa Adélia, 166, Santo André, SP CEP 09210-170 Brazil.

ABSTRACT

Background: Considering the similarity between the testis-specific isoform of angiotensin-converting enzyme and the C-terminal catalytic domain of somatic ACE as well as the structural and functional variability of its natural inhibitors, known as bradykinin-potentiating peptides (BPPs), the effects of different synthetic peptides, BPP-10c (

Methods: The adult animals received either one of the synthetic peptides or captopril (120 nmol/dose per testis) via injection into the testicular parenchyma. After seven days, the mice were sacrificed, and the testes were collected for histopathological evaluation.

Results: BPP-10c and BPP-AP showed an intense disruption of the epithelium, presence of atypical multinucleated cells in the lumen and high degree of seminiferous tubule degeneration, especially in BPP-AP-treated animals. In addition, both synthetic peptides led to a significant reduction in the number of spermatocytes and round spermatids in stages I, V and VII/VIII of the seminiferous cycle, thickness of the seminiferous epithelium and diameter of the seminiferous tubule lumen. Interestingly, no morphological or morphometric alterations were observed in animals treated with captopril or BPP-11e.

Conclusions: The major finding of the present study was that the demonstrated effects of BPP-10c and BPP-AP on the seminiferous epithelium are dependent on their primary structure and cannot be extrapolated to other BPPs.

No MeSH data available.


Related in: MedlinePlus

Morphometric aspects of the seminiferous tubules in mice treated with angiotensin-converting enzyme (ACE) inhibitors. a Thickness of the epithelium and diameter of tubules and lumen of the seminiferous tubules of control and treated animals. b Total support capacity of Sertoli cells during stages I, V, VII/VIII and XII of the seminiferous epithelium cycle of control and treated animals. The data are presented as the mean ± SEM. The various letters indicate significant differences between the analyzed groups as determined via one-way ANOVA followed by the Tukey test as a post hoc test (a, p < 0.001; b, p < 0.0001). C, control; CAP, captopril; (inv)10c, containing the inverted BPP-10c sequence; 10c, BPP-10c; 11e, BPP-11e; AP, BPP-AP
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Fig2: Morphometric aspects of the seminiferous tubules in mice treated with angiotensin-converting enzyme (ACE) inhibitors. a Thickness of the epithelium and diameter of tubules and lumen of the seminiferous tubules of control and treated animals. b Total support capacity of Sertoli cells during stages I, V, VII/VIII and XII of the seminiferous epithelium cycle of control and treated animals. The data are presented as the mean ± SEM. The various letters indicate significant differences between the analyzed groups as determined via one-way ANOVA followed by the Tukey test as a post hoc test (a, p < 0.001; b, p < 0.0001). C, control; CAP, captopril; (inv)10c, containing the inverted BPP-10c sequence; 10c, BPP-10c; 11e, BPP-11e; AP, BPP-AP

Mentions: BPP-10c treatment displayed a reduction in the thickness of the seminiferous epithelium and an increase in the diameter of the seminiferous tubule lumen compared with the (inv)BPP-10c, BPP-11e, captopril and control groups (Fig. 2 –a). Treatment with BPP-AP led to a significant reduction in the thickness of the seminiferous epithelium and an increase in the diameter of the seminiferous tubule lumen compared with the BPP-11e, captopril and control groups (Fig. 2 – a). No alteration was detected in the diameter of tubules in all treatment groups.Fig. 2


Angiotensin-converting enzyme inhibitors of Bothrops jararaca snake venom affect the structure of mice seminiferous epithelium.

Alberto-Silva C, Gilio JM, Portaro FC, Querobino SM, Camargo AC - J Venom Anim Toxins Incl Trop Dis (2015)

Morphometric aspects of the seminiferous tubules in mice treated with angiotensin-converting enzyme (ACE) inhibitors. a Thickness of the epithelium and diameter of tubules and lumen of the seminiferous tubules of control and treated animals. b Total support capacity of Sertoli cells during stages I, V, VII/VIII and XII of the seminiferous epithelium cycle of control and treated animals. The data are presented as the mean ± SEM. The various letters indicate significant differences between the analyzed groups as determined via one-way ANOVA followed by the Tukey test as a post hoc test (a, p < 0.001; b, p < 0.0001). C, control; CAP, captopril; (inv)10c, containing the inverted BPP-10c sequence; 10c, BPP-10c; 11e, BPP-11e; AP, BPP-AP
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4524108&req=5

Fig2: Morphometric aspects of the seminiferous tubules in mice treated with angiotensin-converting enzyme (ACE) inhibitors. a Thickness of the epithelium and diameter of tubules and lumen of the seminiferous tubules of control and treated animals. b Total support capacity of Sertoli cells during stages I, V, VII/VIII and XII of the seminiferous epithelium cycle of control and treated animals. The data are presented as the mean ± SEM. The various letters indicate significant differences between the analyzed groups as determined via one-way ANOVA followed by the Tukey test as a post hoc test (a, p < 0.001; b, p < 0.0001). C, control; CAP, captopril; (inv)10c, containing the inverted BPP-10c sequence; 10c, BPP-10c; 11e, BPP-11e; AP, BPP-AP
Mentions: BPP-10c treatment displayed a reduction in the thickness of the seminiferous epithelium and an increase in the diameter of the seminiferous tubule lumen compared with the (inv)BPP-10c, BPP-11e, captopril and control groups (Fig. 2 –a). Treatment with BPP-AP led to a significant reduction in the thickness of the seminiferous epithelium and an increase in the diameter of the seminiferous tubule lumen compared with the BPP-11e, captopril and control groups (Fig. 2 – a). No alteration was detected in the diameter of tubules in all treatment groups.Fig. 2

Bottom Line: In addition, both synthetic peptides led to a significant reduction in the number of spermatocytes and round spermatids in stages I, V and VII/VIII of the seminiferous cycle, thickness of the seminiferous epithelium and diameter of the seminiferous tubule lumen.Interestingly, no morphological or morphometric alterations were observed in animals treated with captopril or BPP-11e.The major finding of the present study was that the demonstrated effects of BPP-10c and BPP-AP on the seminiferous epithelium are dependent on their primary structure and cannot be extrapolated to other BPPs.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural and Humanities Sciences (CCNH), Federal University of ABC (UFABC), R. Santa Adélia, 166, Santo André, SP CEP 09210-170 Brazil.

ABSTRACT

Background: Considering the similarity between the testis-specific isoform of angiotensin-converting enzyme and the C-terminal catalytic domain of somatic ACE as well as the structural and functional variability of its natural inhibitors, known as bradykinin-potentiating peptides (BPPs), the effects of different synthetic peptides, BPP-10c (

Methods: The adult animals received either one of the synthetic peptides or captopril (120 nmol/dose per testis) via injection into the testicular parenchyma. After seven days, the mice were sacrificed, and the testes were collected for histopathological evaluation.

Results: BPP-10c and BPP-AP showed an intense disruption of the epithelium, presence of atypical multinucleated cells in the lumen and high degree of seminiferous tubule degeneration, especially in BPP-AP-treated animals. In addition, both synthetic peptides led to a significant reduction in the number of spermatocytes and round spermatids in stages I, V and VII/VIII of the seminiferous cycle, thickness of the seminiferous epithelium and diameter of the seminiferous tubule lumen. Interestingly, no morphological or morphometric alterations were observed in animals treated with captopril or BPP-11e.

Conclusions: The major finding of the present study was that the demonstrated effects of BPP-10c and BPP-AP on the seminiferous epithelium are dependent on their primary structure and cannot be extrapolated to other BPPs.

No MeSH data available.


Related in: MedlinePlus