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Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection.

Yu Q, Xiong Y, Gao H, Liu J, Chen Z, Wang Q, Wen D - Virol. J. (2015)

Bottom Line: However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance.The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. qianyucn@126.com.

ABSTRACT

Background: Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.

Methods: In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.

Results: A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.

Conclusions: The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.

No MeSH data available.


Related in: MedlinePlus

Representative KEGG pathway for carbon metabolism. The differentially expressed proteins were mapped as green
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Fig4: Representative KEGG pathway for carbon metabolism. The differentially expressed proteins were mapped as green

Mentions: For protein processing in the endoplasmic reticulum, AcMNPV is entirely dependent on the Sf9 cell translational machinery. We found that the expression of Calreticulin, EIF2α, Heat shock protein 90β and glycoprotein glucosyltransferase, among others, in Sf9 cells which are closely related to protein translation were significantly changed after AcMNPV infection. These proteins are involved in the translation of AcMNPV proteins using the Sf9 cell translation system. Concerning carbon metabolism, the gluconeogenesis/glycolysis pathways held the largest number of proteins. As no gene for an enzyme involved in energy metabolism has been found in any sequenced baculovirus, AcMNPV have to rely on Sf9 cell enzymes for energy metabolism (Fig. 5). The proteins with differential abundances involved in protein processing, carbon metabolism and ribosome were searched in KEGG (http://www.genome.jp/kegg/) and colored in Fig. 3, Fig. 4 and Fig. 5, respectively.Fig. 3


Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection.

Yu Q, Xiong Y, Gao H, Liu J, Chen Z, Wang Q, Wen D - Virol. J. (2015)

Representative KEGG pathway for carbon metabolism. The differentially expressed proteins were mapped as green
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4524103&req=5

Fig4: Representative KEGG pathway for carbon metabolism. The differentially expressed proteins were mapped as green
Mentions: For protein processing in the endoplasmic reticulum, AcMNPV is entirely dependent on the Sf9 cell translational machinery. We found that the expression of Calreticulin, EIF2α, Heat shock protein 90β and glycoprotein glucosyltransferase, among others, in Sf9 cells which are closely related to protein translation were significantly changed after AcMNPV infection. These proteins are involved in the translation of AcMNPV proteins using the Sf9 cell translation system. Concerning carbon metabolism, the gluconeogenesis/glycolysis pathways held the largest number of proteins. As no gene for an enzyme involved in energy metabolism has been found in any sequenced baculovirus, AcMNPV have to rely on Sf9 cell enzymes for energy metabolism (Fig. 5). The proteins with differential abundances involved in protein processing, carbon metabolism and ribosome were searched in KEGG (http://www.genome.jp/kegg/) and colored in Fig. 3, Fig. 4 and Fig. 5, respectively.Fig. 3

Bottom Line: However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance.The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.

View Article: PubMed Central - PubMed

Affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangdong, 510225, People's Republic of China. qianyucn@126.com.

ABSTRACT

Background: Increasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.

Methods: In this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.

Results: A total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.

Conclusions: The determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.

No MeSH data available.


Related in: MedlinePlus