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Applying Advanced Imaging Techniques to a Murine Model of Orthotopic Osteosarcoma.

Broadhead ML, Lokmic Z, Tan ML, Stevenson A, Binns DS, Cullinane C, Hicks RJ, Choong PF, Myers DE - Front Surg (2015)

Bottom Line: Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity.Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET. [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease.Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, St. Vincent's Hospital Melbourne, University of Melbourne , Fitzroy, VIC , Australia.

ABSTRACT

Introduction: Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease.

Materials and methods: Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET.

Results: [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease. Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (pā€‰<ā€‰0.05) by the study endpoint, respectively. In vivo bioluminescent assay showed that systemically delivered PEDF inhibited growth of primary osteosarcoma.

Discussion: Application of [(18)F]-Fluoride-PET and [(18)F]-FDG-PET to an established murine model of orthotopic osteosarcoma has improved the assessment of disease. The use of targeted imaging should prove beneficial for the evaluation of new approaches to osteosarcoma therapy.

No MeSH data available.


Related in: MedlinePlus

Detection of pulmonary metastases by in vivo bioluminescent assay. Shielding was applied to the hind limbs of mice before imaging of the thorax. This allowed pulmonary metastatic disease to be identified. Total flux (p/s) for the region of interest (ROI) was determined at day 27 and 31. Photomicrographs taken at day 31 are shown. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals.
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Figure 5: Detection of pulmonary metastases by in vivo bioluminescent assay. Shielding was applied to the hind limbs of mice before imaging of the thorax. This allowed pulmonary metastatic disease to be identified. Total flux (p/s) for the region of interest (ROI) was determined at day 27 and 31. Photomicrographs taken at day 31 are shown. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals.

Mentions: At day 27 of the study, a total of 16 mice showed pulmonary metastases on in vivo bioluminescent assay. By day 31, pulmonary metastatic disease could be identified in a further seven mice. At day 31 of the study, six mice receiving water as control showed evidence of pulmonary metastatic disease compared to two mice receiving PEDF. For those animals that did possess metastases at day 27 and 31, the mean total flux for pulmonary metastases was determined. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals (Figure 5).


Applying Advanced Imaging Techniques to a Murine Model of Orthotopic Osteosarcoma.

Broadhead ML, Lokmic Z, Tan ML, Stevenson A, Binns DS, Cullinane C, Hicks RJ, Choong PF, Myers DE - Front Surg (2015)

Detection of pulmonary metastases by in vivo bioluminescent assay. Shielding was applied to the hind limbs of mice before imaging of the thorax. This allowed pulmonary metastatic disease to be identified. Total flux (p/s) for the region of interest (ROI) was determined at day 27 and 31. Photomicrographs taken at day 31 are shown. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522961&req=5

Figure 5: Detection of pulmonary metastases by in vivo bioluminescent assay. Shielding was applied to the hind limbs of mice before imaging of the thorax. This allowed pulmonary metastatic disease to be identified. Total flux (p/s) for the region of interest (ROI) was determined at day 27 and 31. Photomicrographs taken at day 31 are shown. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals.
Mentions: At day 27 of the study, a total of 16 mice showed pulmonary metastases on in vivo bioluminescent assay. By day 31, pulmonary metastatic disease could be identified in a further seven mice. At day 31 of the study, six mice receiving water as control showed evidence of pulmonary metastatic disease compared to two mice receiving PEDF. For those animals that did possess metastases at day 27 and 31, the mean total flux for pulmonary metastases was determined. Treatment with PEDF did not affect the mean burden of pulmonary metastases in these animals (Figure 5).

Bottom Line: Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity.Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET. [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease.Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, St. Vincent's Hospital Melbourne, University of Melbourne , Fitzroy, VIC , Australia.

ABSTRACT

Introduction: Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease.

Materials and methods: Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET.

Results: [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease. Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (pā€‰<ā€‰0.05) by the study endpoint, respectively. In vivo bioluminescent assay showed that systemically delivered PEDF inhibited growth of primary osteosarcoma.

Discussion: Application of [(18)F]-Fluoride-PET and [(18)F]-FDG-PET to an established murine model of orthotopic osteosarcoma has improved the assessment of disease. The use of targeted imaging should prove beneficial for the evaluation of new approaches to osteosarcoma therapy.

No MeSH data available.


Related in: MedlinePlus