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Applying Advanced Imaging Techniques to a Murine Model of Orthotopic Osteosarcoma.

Broadhead ML, Lokmic Z, Tan ML, Stevenson A, Binns DS, Cullinane C, Hicks RJ, Choong PF, Myers DE - Front Surg (2015)

Bottom Line: Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity.Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET. [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease.Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, St. Vincent's Hospital Melbourne, University of Melbourne , Fitzroy, VIC , Australia.

ABSTRACT

Introduction: Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease.

Materials and methods: Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET.

Results: [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease. Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively. In vivo bioluminescent assay showed that systemically delivered PEDF inhibited growth of primary osteosarcoma.

Discussion: Application of [(18)F]-Fluoride-PET and [(18)F]-FDG-PET to an established murine model of orthotopic osteosarcoma has improved the assessment of disease. The use of targeted imaging should prove beneficial for the evaluation of new approaches to osteosarcoma therapy.

No MeSH data available.


Related in: MedlinePlus

Primary tumor growth after treatment by in vivo bioluminescent assay. Total flux (p/s) for the primary tumor region of interest (ROI 1) was determined at day 20, 24, 27, and 31. Primary tumor total flux is reduced by PEDF treatment at day 31 (*p < 0.001). Photomicrographs taken at day 31 are shown.
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Figure 3: Primary tumor growth after treatment by in vivo bioluminescent assay. Total flux (p/s) for the primary tumor region of interest (ROI 1) was determined at day 20, 24, 27, and 31. Primary tumor total flux is reduced by PEDF treatment at day 31 (*p < 0.001). Photomicrographs taken at day 31 are shown.

Mentions: Treatment with PEDF was started at day 20. Average tumor volume at day 20 was 7.50 ± 1.48mm3. Treatment with PEDF caused a significant reduction in tumor growth by day 31 of the study when compared to the group receiving sterile water. Primary tumor total flux was reduced by 51.21% (p < 0.001) at day 31 (Figure 3). Tumor volume, as calculated from anterioposterior and lateral dimensions, from day 20 to the study endpoint, showed no significant difference between treatment groups (Table 1).


Applying Advanced Imaging Techniques to a Murine Model of Orthotopic Osteosarcoma.

Broadhead ML, Lokmic Z, Tan ML, Stevenson A, Binns DS, Cullinane C, Hicks RJ, Choong PF, Myers DE - Front Surg (2015)

Primary tumor growth after treatment by in vivo bioluminescent assay. Total flux (p/s) for the primary tumor region of interest (ROI 1) was determined at day 20, 24, 27, and 31. Primary tumor total flux is reduced by PEDF treatment at day 31 (*p < 0.001). Photomicrographs taken at day 31 are shown.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4522961&req=5

Figure 3: Primary tumor growth after treatment by in vivo bioluminescent assay. Total flux (p/s) for the primary tumor region of interest (ROI 1) was determined at day 20, 24, 27, and 31. Primary tumor total flux is reduced by PEDF treatment at day 31 (*p < 0.001). Photomicrographs taken at day 31 are shown.
Mentions: Treatment with PEDF was started at day 20. Average tumor volume at day 20 was 7.50 ± 1.48mm3. Treatment with PEDF caused a significant reduction in tumor growth by day 31 of the study when compared to the group receiving sterile water. Primary tumor total flux was reduced by 51.21% (p < 0.001) at day 31 (Figure 3). Tumor volume, as calculated from anterioposterior and lateral dimensions, from day 20 to the study endpoint, showed no significant difference between treatment groups (Table 1).

Bottom Line: Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity.Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET. [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease.Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, St. Vincent's Hospital Melbourne, University of Melbourne , Fitzroy, VIC , Australia.

ABSTRACT

Introduction: Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease.

Materials and methods: Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET.

Results: [(18)F]-Fluoride-PET was more sensitive than [(18)F]-FDG-PET for detecting early disease. Both [(18)F]-Fluoride-PET and [(18)F]-FDG-PET showed progressive disease in the model, with fourfold and twofold increases in standardized uptake value (p < 0.05) by the study endpoint, respectively. In vivo bioluminescent assay showed that systemically delivered PEDF inhibited growth of primary osteosarcoma.

Discussion: Application of [(18)F]-Fluoride-PET and [(18)F]-FDG-PET to an established murine model of orthotopic osteosarcoma has improved the assessment of disease. The use of targeted imaging should prove beneficial for the evaluation of new approaches to osteosarcoma therapy.

No MeSH data available.


Related in: MedlinePlus