Limits...
Hoarseness as the Presenting Symptom of Visceral Leishmaniasis with Muco-Cutaneous Lesions: A Case Report.

Mortazavi H, Mohebali M, Taslimi Y, Sadeghipour P, Ansari M, Kamyab K, Talebi M, Khamesipour A - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: PCR was performed on the specimens of skin, bone marrow, mucosa, and saliva, the results were positive.The pathogenic agent was identified as Leishmania major by the nested PCR.To the best of our knowledge, probably hoarseness due to L. major has not been previously reported from Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Herein, a 28-year-old man with hoarseness, skin and oral lesions is presented. At the time of admission, the patient had an erythematous plaque on his chin near his lower lip and an erythematous-violaceous plaque on his palate near the opening of the pharynx and 20 kg weight lost in last one year. The biopsy of his skin lesions by hematoxylin and eosin staining revealed an infiltration of the dermis by lymphoplasma and histiocytic cells with a loose granuloma formation suggestive of leishmaniasis. Biopsy of mucosal lesions revealed Leishman bodies in dermis. PCR was performed on the specimens of skin, bone marrow, mucosa, and saliva, the results were positive. The pathogenic agent was identified as Leishmania major by the nested PCR. Serologic tests including direct agglutination test (DAT) and indirect immunofluorescence test (IFAT) were positive with high titers of anti-L. infantum antibodies (1:102400 versus 1:800, respectively), indicative of visceral involvement. The patient responded to a combination of miltefosine and meglumine antimoniate (Glucantime®). Visceral involvement due to L. major is rarely reported. To the best of our knowledge, probably hoarseness due to L. major has not been previously reported from Iran.

No MeSH data available.


Related in: MedlinePlus

Nested –PCR amplification of KDNA extracted from skin, bone marrow, mucosa and saliva of the patient which then confirmed by sequence analysesLane 1: Molecular weight marker 100bpLane 2: L. tropicaLane 3: Patient sampleLane 4: L. major
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4522308&req=5

Figure 3: Nested –PCR amplification of KDNA extracted from skin, bone marrow, mucosa and saliva of the patient which then confirmed by sequence analysesLane 1: Molecular weight marker 100bpLane 2: L. tropicaLane 3: Patient sampleLane 4: L. major

Mentions: In order to determine the possibility of Leishmania infection, nested PCR method was applied. The primers were already designed by Noyes (6). External primers CSB2XF and CSB1XR in the first round of PCR, and internal primers 13Z and LIR in the second round of PCR were utilized (invitrogen, UK). As a positive control, genomic DNAs of standard L. major (MRHOM/IR/75/ER) and L. tropica (MOHM/IR/09/Khamesipour-Mashhad) were used in parallel. The PCR products were analyzed by 1.5% agarose gel electrophoresis. The result of the nested PCR is shown in Fig. 3.


Hoarseness as the Presenting Symptom of Visceral Leishmaniasis with Muco-Cutaneous Lesions: A Case Report.

Mortazavi H, Mohebali M, Taslimi Y, Sadeghipour P, Ansari M, Kamyab K, Talebi M, Khamesipour A - Iran J Parasitol (2015 Apr-Jun)

Nested –PCR amplification of KDNA extracted from skin, bone marrow, mucosa and saliva of the patient which then confirmed by sequence analysesLane 1: Molecular weight marker 100bpLane 2: L. tropicaLane 3: Patient sampleLane 4: L. major
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522308&req=5

Figure 3: Nested –PCR amplification of KDNA extracted from skin, bone marrow, mucosa and saliva of the patient which then confirmed by sequence analysesLane 1: Molecular weight marker 100bpLane 2: L. tropicaLane 3: Patient sampleLane 4: L. major
Mentions: In order to determine the possibility of Leishmania infection, nested PCR method was applied. The primers were already designed by Noyes (6). External primers CSB2XF and CSB1XR in the first round of PCR, and internal primers 13Z and LIR in the second round of PCR were utilized (invitrogen, UK). As a positive control, genomic DNAs of standard L. major (MRHOM/IR/75/ER) and L. tropica (MOHM/IR/09/Khamesipour-Mashhad) were used in parallel. The PCR products were analyzed by 1.5% agarose gel electrophoresis. The result of the nested PCR is shown in Fig. 3.

Bottom Line: PCR was performed on the specimens of skin, bone marrow, mucosa, and saliva, the results were positive.The pathogenic agent was identified as Leishmania major by the nested PCR.To the best of our knowledge, probably hoarseness due to L. major has not been previously reported from Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT
Herein, a 28-year-old man with hoarseness, skin and oral lesions is presented. At the time of admission, the patient had an erythematous plaque on his chin near his lower lip and an erythematous-violaceous plaque on his palate near the opening of the pharynx and 20 kg weight lost in last one year. The biopsy of his skin lesions by hematoxylin and eosin staining revealed an infiltration of the dermis by lymphoplasma and histiocytic cells with a loose granuloma formation suggestive of leishmaniasis. Biopsy of mucosal lesions revealed Leishman bodies in dermis. PCR was performed on the specimens of skin, bone marrow, mucosa, and saliva, the results were positive. The pathogenic agent was identified as Leishmania major by the nested PCR. Serologic tests including direct agglutination test (DAT) and indirect immunofluorescence test (IFAT) were positive with high titers of anti-L. infantum antibodies (1:102400 versus 1:800, respectively), indicative of visceral involvement. The patient responded to a combination of miltefosine and meglumine antimoniate (Glucantime®). Visceral involvement due to L. major is rarely reported. To the best of our knowledge, probably hoarseness due to L. major has not been previously reported from Iran.

No MeSH data available.


Related in: MedlinePlus