Limits...
Histological Changes of the Ovary in Pregnant Mice Vaginally Exposed to Toxoplasma gondii.

Eslamirad Z, Bayat PD, Babaei S - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: Ovaries and uterine horns of animals were removed and prepared for light microscopy.In ovaries of experimental groups, changes of corpus luteum were observed.The comparison of experimental and control groups revealed that the number of primary follicles, secondary follicle, atretic primary follicles and atretic secondary follicles had significant differences (P≤0.001).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

ABSTRACT

Background: Congenital toxoplasmosis is one cause of abortion. Infection can disrupt ovarian cycles and because toxoplasmosis is an infectious disease may have a similar effect on the ovaries. The purpose of this study was to investigate the pathological changes in the ovaries due to toxoplasmosis.

Methods: Tachyzoites of Toxoplasma gondii were harvested from peritoneal fluid of mice, experimentally infected. Two females and one male mouse were housed per cage for mating in the overnight. The pregnant mice were divided into experimental and control groups. Experimental group were infected by parasite but the control group received the normal saline. The experimental and control mice were euthanized. Ovaries and uterine horns of animals were removed and prepared for light microscopy.

Results: Ovaries of infected pregnant mice presented gross morphological differences compared to the control groups. In ovaries of experimental groups, changes of corpus luteum were observed. The comparison of experimental and control groups revealed that the number of primary follicles, secondary follicle, atretic primary follicles and atretic secondary follicles had significant differences (P≤0.001).

Conclusion: Toxoplasma gondii alters ovarian follicular growth and development in mice. In addition, it alters number of different phases of follicles and corpus luteum in ovaries of mice.

No MeSH data available.


Related in: MedlinePlus

The section of ovary in mice in 16th day of pregnancy. A. Mice ovary in control group (10×10). B. Mice ovary in experimental group (10×10). 1) Corpus luteum, 2) Primary follicle 3) secondary follicle 4) Atretic primary follicles 5) Atretic secondary follicles
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4522304&req=5

Figure 1: The section of ovary in mice in 16th day of pregnancy. A. Mice ovary in control group (10×10). B. Mice ovary in experimental group (10×10). 1) Corpus luteum, 2) Primary follicle 3) secondary follicle 4) Atretic primary follicles 5) Atretic secondary follicles

Mentions: Ovaries of pregnant mice that were vaginally infected by Toxplasma presented gross morphologic differences compared to those of the control groups. The primary and secondary healthy follicles and corpus luteum of the control groups showed normal appearance (Fig. 1A). All ovarian sections in experimental groups showed great defects in follicles, atretic follicles and corpus luteum in developmental stages (Fig. 1B). Furthermore, in these groups some of the primary follicles showed normal appearance, although with high congestion compared to the control group (Fig. 1B).


Histological Changes of the Ovary in Pregnant Mice Vaginally Exposed to Toxoplasma gondii.

Eslamirad Z, Bayat PD, Babaei S - Iran J Parasitol (2015 Apr-Jun)

The section of ovary in mice in 16th day of pregnancy. A. Mice ovary in control group (10×10). B. Mice ovary in experimental group (10×10). 1) Corpus luteum, 2) Primary follicle 3) secondary follicle 4) Atretic primary follicles 5) Atretic secondary follicles
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522304&req=5

Figure 1: The section of ovary in mice in 16th day of pregnancy. A. Mice ovary in control group (10×10). B. Mice ovary in experimental group (10×10). 1) Corpus luteum, 2) Primary follicle 3) secondary follicle 4) Atretic primary follicles 5) Atretic secondary follicles
Mentions: Ovaries of pregnant mice that were vaginally infected by Toxplasma presented gross morphologic differences compared to those of the control groups. The primary and secondary healthy follicles and corpus luteum of the control groups showed normal appearance (Fig. 1A). All ovarian sections in experimental groups showed great defects in follicles, atretic follicles and corpus luteum in developmental stages (Fig. 1B). Furthermore, in these groups some of the primary follicles showed normal appearance, although with high congestion compared to the control group (Fig. 1B).

Bottom Line: Ovaries and uterine horns of animals were removed and prepared for light microscopy.In ovaries of experimental groups, changes of corpus luteum were observed.The comparison of experimental and control groups revealed that the number of primary follicles, secondary follicle, atretic primary follicles and atretic secondary follicles had significant differences (P≤0.001).

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.

ABSTRACT

Background: Congenital toxoplasmosis is one cause of abortion. Infection can disrupt ovarian cycles and because toxoplasmosis is an infectious disease may have a similar effect on the ovaries. The purpose of this study was to investigate the pathological changes in the ovaries due to toxoplasmosis.

Methods: Tachyzoites of Toxoplasma gondii were harvested from peritoneal fluid of mice, experimentally infected. Two females and one male mouse were housed per cage for mating in the overnight. The pregnant mice were divided into experimental and control groups. Experimental group were infected by parasite but the control group received the normal saline. The experimental and control mice were euthanized. Ovaries and uterine horns of animals were removed and prepared for light microscopy.

Results: Ovaries of infected pregnant mice presented gross morphological differences compared to the control groups. In ovaries of experimental groups, changes of corpus luteum were observed. The comparison of experimental and control groups revealed that the number of primary follicles, secondary follicle, atretic primary follicles and atretic secondary follicles had significant differences (P≤0.001).

Conclusion: Toxoplasma gondii alters ovarian follicular growth and development in mice. In addition, it alters number of different phases of follicles and corpus luteum in ovaries of mice.

No MeSH data available.


Related in: MedlinePlus