Limits...
Evaluation of Apoptotic and Antileishmanial Activities of Artemisinin on Promastigotes and BALB/C Mice Infected with Leishmania major.

Ghaffarifar F, Esavand Heydari F, Dalimi A, Hassan ZM, Delavari M, Mikaeiloo H - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05).Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions.Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: In leishmaniasis, some drugs prescribed for treatment have toxic effects and there are reports about drug resistance in some countries. Due to this fact, using herbal drugs such as artemisinin with good efficacy and low toxic effect might be suitable.

Methods: We evaluated the apoptotic effect of artemisinin on Leishmania major in vitro and the antileishmanial activities of artemisinin on leishmaniasis in BALB/c mice and at the end INF-γ and IL-4 cytokines levels were detected by ELISA in spleen cell culture supernatants. During treatment the lesion size and survival rate were measured each four and ten days, respectively.

Results: Percentage of early and late apoptosis in promastigotes of control group and promastigotes treated with 10, 25, 50 and 100 μg/ml of artemisinin after 48 h were 0.13, 16.04, 41.23, 49.03 and 81.83, respectively. The IFN-γ in ointment treated group were higher than those of other groups (P<0.05). The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05). The survival rate of mice 150 days after challenge in treated group with ointment of artemisinin was 66% while all mice in control groups were died.

Conclusion: All of in vitro results represented that this drug had antileishmanial effects and these results were confirmed by evaluation effects in vivo condition of leishmaniasis. Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions. Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

No MeSH data available.


Related in: MedlinePlus

Percentage of survival rate of infected BALB/c mice with 2×106 in treated groups with ointment and intraperitoneal injection form of artemisinin and control goups during 150 days after challenge. The number of mice in each group is six
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4522302&req=5

Figure 4: Percentage of survival rate of infected BALB/c mice with 2×106 in treated groups with ointment and intraperitoneal injection form of artemisinin and control goups during 150 days after challenge. The number of mice in each group is six

Mentions: For evaluation of survival rate, six mice in each group were treated then followed for 150 days. The results are shown in Fig. 4. The higher rate is related to a group that treated with ointment of artemisinin.


Evaluation of Apoptotic and Antileishmanial Activities of Artemisinin on Promastigotes and BALB/C Mice Infected with Leishmania major.

Ghaffarifar F, Esavand Heydari F, Dalimi A, Hassan ZM, Delavari M, Mikaeiloo H - Iran J Parasitol (2015 Apr-Jun)

Percentage of survival rate of infected BALB/c mice with 2×106 in treated groups with ointment and intraperitoneal injection form of artemisinin and control goups during 150 days after challenge. The number of mice in each group is six
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522302&req=5

Figure 4: Percentage of survival rate of infected BALB/c mice with 2×106 in treated groups with ointment and intraperitoneal injection form of artemisinin and control goups during 150 days after challenge. The number of mice in each group is six
Mentions: For evaluation of survival rate, six mice in each group were treated then followed for 150 days. The results are shown in Fig. 4. The higher rate is related to a group that treated with ointment of artemisinin.

Bottom Line: The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05).Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions.Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: In leishmaniasis, some drugs prescribed for treatment have toxic effects and there are reports about drug resistance in some countries. Due to this fact, using herbal drugs such as artemisinin with good efficacy and low toxic effect might be suitable.

Methods: We evaluated the apoptotic effect of artemisinin on Leishmania major in vitro and the antileishmanial activities of artemisinin on leishmaniasis in BALB/c mice and at the end INF-γ and IL-4 cytokines levels were detected by ELISA in spleen cell culture supernatants. During treatment the lesion size and survival rate were measured each four and ten days, respectively.

Results: Percentage of early and late apoptosis in promastigotes of control group and promastigotes treated with 10, 25, 50 and 100 μg/ml of artemisinin after 48 h were 0.13, 16.04, 41.23, 49.03 and 81.83, respectively. The IFN-γ in ointment treated group were higher than those of other groups (P<0.05). The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05). The survival rate of mice 150 days after challenge in treated group with ointment of artemisinin was 66% while all mice in control groups were died.

Conclusion: All of in vitro results represented that this drug had antileishmanial effects and these results were confirmed by evaluation effects in vivo condition of leishmaniasis. Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions. Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

No MeSH data available.


Related in: MedlinePlus