Limits...
Evaluation of Apoptotic and Antileishmanial Activities of Artemisinin on Promastigotes and BALB/C Mice Infected with Leishmania major.

Ghaffarifar F, Esavand Heydari F, Dalimi A, Hassan ZM, Delavari M, Mikaeiloo H - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05).Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions.Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: In leishmaniasis, some drugs prescribed for treatment have toxic effects and there are reports about drug resistance in some countries. Due to this fact, using herbal drugs such as artemisinin with good efficacy and low toxic effect might be suitable.

Methods: We evaluated the apoptotic effect of artemisinin on Leishmania major in vitro and the antileishmanial activities of artemisinin on leishmaniasis in BALB/c mice and at the end INF-γ and IL-4 cytokines levels were detected by ELISA in spleen cell culture supernatants. During treatment the lesion size and survival rate were measured each four and ten days, respectively.

Results: Percentage of early and late apoptosis in promastigotes of control group and promastigotes treated with 10, 25, 50 and 100 μg/ml of artemisinin after 48 h were 0.13, 16.04, 41.23, 49.03 and 81.83, respectively. The IFN-γ in ointment treated group were higher than those of other groups (P<0.05). The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05). The survival rate of mice 150 days after challenge in treated group with ointment of artemisinin was 66% while all mice in control groups were died.

Conclusion: All of in vitro results represented that this drug had antileishmanial effects and these results were confirmed by evaluation effects in vivo condition of leishmaniasis. Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions. Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

No MeSH data available.


Related in: MedlinePlus

Mean and SD of promastigotes of Leishmania major cultured with 5, 10, 25, 50, and 100 μg/ml concentrations of artemisininat 24, 48 and 72h and comparing them with control group. There are significant differences between the control group and treated groups in 24, 48 and 72 h (P<0.05)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4522302&req=5

Figure 1: Mean and SD of promastigotes of Leishmania major cultured with 5, 10, 25, 50, and 100 μg/ml concentrations of artemisininat 24, 48 and 72h and comparing them with control group. There are significant differences between the control group and treated groups in 24, 48 and 72 h (P<0.05)

Mentions: The cytotoxic effect of artemisinin on promastigotes was observed. These result show artemisinin has antileishmanial effects on promastigotes. There were significant differences with control group and treated groups in 24, 48 and 72 h (P<0.05) (Fig. 1).


Evaluation of Apoptotic and Antileishmanial Activities of Artemisinin on Promastigotes and BALB/C Mice Infected with Leishmania major.

Ghaffarifar F, Esavand Heydari F, Dalimi A, Hassan ZM, Delavari M, Mikaeiloo H - Iran J Parasitol (2015 Apr-Jun)

Mean and SD of promastigotes of Leishmania major cultured with 5, 10, 25, 50, and 100 μg/ml concentrations of artemisininat 24, 48 and 72h and comparing them with control group. There are significant differences between the control group and treated groups in 24, 48 and 72 h (P<0.05)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522302&req=5

Figure 1: Mean and SD of promastigotes of Leishmania major cultured with 5, 10, 25, 50, and 100 μg/ml concentrations of artemisininat 24, 48 and 72h and comparing them with control group. There are significant differences between the control group and treated groups in 24, 48 and 72 h (P<0.05)
Mentions: The cytotoxic effect of artemisinin on promastigotes was observed. These result show artemisinin has antileishmanial effects on promastigotes. There were significant differences with control group and treated groups in 24, 48 and 72 h (P<0.05) (Fig. 1).

Bottom Line: The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05).Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions.Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: In leishmaniasis, some drugs prescribed for treatment have toxic effects and there are reports about drug resistance in some countries. Due to this fact, using herbal drugs such as artemisinin with good efficacy and low toxic effect might be suitable.

Methods: We evaluated the apoptotic effect of artemisinin on Leishmania major in vitro and the antileishmanial activities of artemisinin on leishmaniasis in BALB/c mice and at the end INF-γ and IL-4 cytokines levels were detected by ELISA in spleen cell culture supernatants. During treatment the lesion size and survival rate were measured each four and ten days, respectively.

Results: Percentage of early and late apoptosis in promastigotes of control group and promastigotes treated with 10, 25, 50 and 100 μg/ml of artemisinin after 48 h were 0.13, 16.04, 41.23, 49.03 and 81.83, respectively. The IFN-γ in ointment treated group were higher than those of other groups (P<0.05). The in vivo results showed that ointment compounds healed the lesions more effectively rather than intraperitoneal injection method (P<0.05). The survival rate of mice 150 days after challenge in treated group with ointment of artemisinin was 66% while all mice in control groups were died.

Conclusion: All of in vitro results represented that this drug had antileishmanial effects and these results were confirmed by evaluation effects in vivo condition of leishmaniasis. Interestingly, according to these results it can be concluded that this drug has antileishmanial effects in vitro and in vivo conditions. Artemisinin induces cytotoxic effect on L. major via apoptosis-related mechanism.

No MeSH data available.


Related in: MedlinePlus