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Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.

Mahmoudi MR, Kazemi B, Haghighi A, Karanis P - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively.Totally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively.For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Microbiology and Parasitology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

ABSTRACT

Background: Free-living amoebae such as Acanthamoeba species may act as carriers of Cryptosporidium and Toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these parasites. In the present study, a loop mediated isothermal amplification (LAMP) method for detection of Toxoplasma and a PCR assay were developed for investigation of Acanthamoeba in environmental water samples.

Methods: A total of 34 samples were collected from the surface water in Guilan Province. Water samples were filtrated with membrane filters and followed by DNA extraction. PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively.

Results: Totally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively. Two samples were positive for both investigated parasites.

Conclusion: The investigated water supplies, are contaminated by Toxoplasma and Acanthamoeba (oo)cystes. Acanthamoeba may play an important role in water-borne transmission of Toxoplasma in the study area. For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.

No MeSH data available.


Related in: MedlinePlus

Map of Guilan and study area
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Figure 1: Map of Guilan and study area

Mentions: All rivers investigated in present study have different uses including agriculture, industry, residential and recreational activities. The rivers are popular as weekend summer resorts, too. Thirty four water samples were collected during 2009 to 2010 from the river waters in Guilan Province (north of Iran). Some of the sampling sites chosen for sampling in this study were along the Sefidrood River, in different cities of Guilan (Fig. 1), and some samples collected from others surface waters including rivers, dams and lagoons in Guilan (Table 1).


Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.

Mahmoudi MR, Kazemi B, Haghighi A, Karanis P - Iran J Parasitol (2015 Apr-Jun)

Map of Guilan and study area
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522301&req=5

Figure 1: Map of Guilan and study area
Mentions: All rivers investigated in present study have different uses including agriculture, industry, residential and recreational activities. The rivers are popular as weekend summer resorts, too. Thirty four water samples were collected during 2009 to 2010 from the river waters in Guilan Province (north of Iran). Some of the sampling sites chosen for sampling in this study were along the Sefidrood River, in different cities of Guilan (Fig. 1), and some samples collected from others surface waters including rivers, dams and lagoons in Guilan (Table 1).

Bottom Line: PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively.Totally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively.For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Microbiology and Parasitology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

ABSTRACT

Background: Free-living amoebae such as Acanthamoeba species may act as carriers of Cryptosporidium and Toxoplasma oocysts, thus, may play an important role in the water-borne transmission of these parasites. In the present study, a loop mediated isothermal amplification (LAMP) method for detection of Toxoplasma and a PCR assay were developed for investigation of Acanthamoeba in environmental water samples.

Methods: A total of 34 samples were collected from the surface water in Guilan Province. Water samples were filtrated with membrane filters and followed by DNA extraction. PCR and LAMP methods used for detection of the protozoan parasites Acanthamoeba and Toxoplasma respectively.

Results: Totally 30 and 2 of 34 samples were positive for Acanthamoeba and Toxoplasma oocysts respectively. Two samples were positive for both investigated parasites.

Conclusion: The investigated water supplies, are contaminated by Toxoplasma and Acanthamoeba (oo)cystes. Acanthamoeba may play an important role in water-borne transmission of Toxoplasma in the study area. For the first time in Iran, protocol of LAMP method was used effectively for the detection of Toxoplasma in surface water samples in Iran.

No MeSH data available.


Related in: MedlinePlus