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Conjugated Linoleic Acid Stimulates Apoptosis in RH and Tehran Strains of Toxoplasma gondii, in Vitro.

Shamseddin J, Akhlaghi L, Razmjou E, Shojaee S, Monavari SH, Tajik N, Ebrahimi SA, Meamar AR - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: The results of MTT indicated that CLA could decrease the proliferation of tachyzoites of both strains in HeLa cells.Conjugated linoleic acid has anti-toxoplasmacidal activity on tachyzoites of T. gondii.Therefore, we recommended further studies on this component in order to achieve a new drug against the parasite.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: The aim of this study was to evaluate the effects of conjugated linoleic acid (CLA) on apoptosis of tachyzoites of T. gondii, RH strain (type I) and the cyst-forming Tehran strain (type II) in vitro.

Methods: Toxoplasma strains were injected into the peritoneal cavity of BALB/c mice. The Tehran strain forms cysts in the brain of mice. Bradyzoites within the cysts are reactivated to proliferative tachyzoites, by dexamethasone. Tachyzoites were aspirated from the peritoneum of infected mice, and the percentage of viable parasites was estimated with trypan blue staining. Tachyzoites were inoculated into HeLa cells cultivated in DMEM medium. Different concentrations of CLA were evaluated on T. gondii in HeLa cells by the tetrazolium (MTT) colorimetric assay. Differentiation between apoptosis and cell death was determined by flow cytometry using Annexin V and propidium iodide (PI) double staining. The statistical analysis performed by GraphPad Prism version 6.00.

Results: CLA induces apoptosis in virulent (RH) and avirulent (Tehran) strains of T. gondii. The results of MTT indicated that CLA could decrease the proliferation of tachyzoites of both strains in HeLa cells.

Conclusion: Conjugated linoleic acid has anti-toxoplasmacidal activity on tachyzoites of T. gondii. Therefore, we recommended further studies on this component in order to achieve a new drug against the parasite.

No MeSH data available.


Related in: MedlinePlus

Effects of various concentrations of conjugated linoleic acid on cell viability of Toxoplasma Tehran (A) and RH (B) strains after 24 (I), 48 (II), and 72 h (III). Data are the mean ± SE from at least three separate experiments (P< 0.0001)
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Figure 1: Effects of various concentrations of conjugated linoleic acid on cell viability of Toxoplasma Tehran (A) and RH (B) strains after 24 (I), 48 (II), and 72 h (III). Data are the mean ± SE from at least three separate experiments (P< 0.0001)

Mentions: Different concentrations of CLA were not affected on non-infected HeLa cells. The effects of CLA on cell viability of RH and Tehran strains of T. gondii, assessed by MTT are shown in Fig 1. Cell proliferation decreased 48 and 72 h post-treatment in RH and Tehran strains of T. gondii (Fig. 1), except in the case of 200 μM CLA at 24 and 48 h in the Tehran strain (Fig. 1A). Cell populations gradually declined in RH compared to the Tehran strain (Fig. 1). The IC50 value, the concentration at which parasite growth was inhibited by 50%, in the Tehran strain 24, 48, and 72 h post-treatment with CLA was 20, 30, and 100 μM, respectively. IC50 in the RH strain after 24 and 48 h was 100 μM and 150 μM after 72 h.


Conjugated Linoleic Acid Stimulates Apoptosis in RH and Tehran Strains of Toxoplasma gondii, in Vitro.

Shamseddin J, Akhlaghi L, Razmjou E, Shojaee S, Monavari SH, Tajik N, Ebrahimi SA, Meamar AR - Iran J Parasitol (2015 Apr-Jun)

Effects of various concentrations of conjugated linoleic acid on cell viability of Toxoplasma Tehran (A) and RH (B) strains after 24 (I), 48 (II), and 72 h (III). Data are the mean ± SE from at least three separate experiments (P< 0.0001)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522299&req=5

Figure 1: Effects of various concentrations of conjugated linoleic acid on cell viability of Toxoplasma Tehran (A) and RH (B) strains after 24 (I), 48 (II), and 72 h (III). Data are the mean ± SE from at least three separate experiments (P< 0.0001)
Mentions: Different concentrations of CLA were not affected on non-infected HeLa cells. The effects of CLA on cell viability of RH and Tehran strains of T. gondii, assessed by MTT are shown in Fig 1. Cell proliferation decreased 48 and 72 h post-treatment in RH and Tehran strains of T. gondii (Fig. 1), except in the case of 200 μM CLA at 24 and 48 h in the Tehran strain (Fig. 1A). Cell populations gradually declined in RH compared to the Tehran strain (Fig. 1). The IC50 value, the concentration at which parasite growth was inhibited by 50%, in the Tehran strain 24, 48, and 72 h post-treatment with CLA was 20, 30, and 100 μM, respectively. IC50 in the RH strain after 24 and 48 h was 100 μM and 150 μM after 72 h.

Bottom Line: The results of MTT indicated that CLA could decrease the proliferation of tachyzoites of both strains in HeLa cells.Conjugated linoleic acid has anti-toxoplasmacidal activity on tachyzoites of T. gondii.Therefore, we recommended further studies on this component in order to achieve a new drug against the parasite.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: The aim of this study was to evaluate the effects of conjugated linoleic acid (CLA) on apoptosis of tachyzoites of T. gondii, RH strain (type I) and the cyst-forming Tehran strain (type II) in vitro.

Methods: Toxoplasma strains were injected into the peritoneal cavity of BALB/c mice. The Tehran strain forms cysts in the brain of mice. Bradyzoites within the cysts are reactivated to proliferative tachyzoites, by dexamethasone. Tachyzoites were aspirated from the peritoneum of infected mice, and the percentage of viable parasites was estimated with trypan blue staining. Tachyzoites were inoculated into HeLa cells cultivated in DMEM medium. Different concentrations of CLA were evaluated on T. gondii in HeLa cells by the tetrazolium (MTT) colorimetric assay. Differentiation between apoptosis and cell death was determined by flow cytometry using Annexin V and propidium iodide (PI) double staining. The statistical analysis performed by GraphPad Prism version 6.00.

Results: CLA induces apoptosis in virulent (RH) and avirulent (Tehran) strains of T. gondii. The results of MTT indicated that CLA could decrease the proliferation of tachyzoites of both strains in HeLa cells.

Conclusion: Conjugated linoleic acid has anti-toxoplasmacidal activity on tachyzoites of T. gondii. Therefore, we recommended further studies on this component in order to achieve a new drug against the parasite.

No MeSH data available.


Related in: MedlinePlus