Limits...
Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces.

Lashkarizadeh MR, Asgaripour K, Saedi Dezaki E, Fasihi Harandi M - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively.In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Surgery is the preferred treatment for hydatid cyst (cystic echinococcosis, CE). At present, various scolicidal agents have been used for inactivation of protoscoleces during surgery, but they are associated with adverse side effects. The aim of the present study was to evaluate the scolicidal effects of amphotricin B, Silver nano particles, Foeniculum vulgare Mill, essential oil and hypertonic saline against protoscoleces of hydatid cyst on an in vitro model.

Methods: Protoscoleces were aseptically aspirated from the naturally infected livers of sheep and goats. Various concentrations of AmB (2.5-20 mg/ml), Ag-NPs (0.5-4 mg/ml), F. vulgare essential oil (0.125-1 mg/ml) and hypertonic saline (10-20%) were used for 5-60 min. Eosin exclusion test was used to determine the viability of protoscoleces.

Results: Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.

Conclusion: The results indicated weak scolicidal activity of AmB and Ag-NPs; whereas F. vulgare essential oil had potent scolicidal activity against protoscoleces of hydatid cyst that revealed the potential of F. vulgare as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

No MeSH data available.


Related in: MedlinePlus

Scolicidal effects of hypertonic saline against protoscoleces of Echinococcus granulosus at various concentrations following different exposure times
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4522296&req=5

Figure 6: Scolicidal effects of hypertonic saline against protoscoleces of Echinococcus granulosus at various concentrations following different exposure times

Mentions: The findings revealed that among tested drugs, F. vulgare essential oil and hypertonic saline had potent scolicidal effects against protoscoleces of hydatid cysts in a dose-dependent and also time-dependent manner, while AmB and Ag-NPs demonstrated weak scolocidal activities on protoscoleces of hydatid cysts (Fig. 3 and 4). F. vulgare essential oil at the concentration of 1 mg/ml after 5 min of exposure killed 100% protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 10 min of exposure to concentration of 0.5 mg/ml was 100%. The scolicidal effect of F. vulgare essential oil at the concentration of 0.25 mg/ml was 11.6, 42.6, 73.3, 100 and 100% after 5, 10, 20, 30 and 60 min of incubation, respectively. These values for the concentration of 0.125 mg/ml were 5, 18.3, 39.6, 57.3 and 73.6% respectively (Fig. 5). In contrast, the maximum protoscolicidal effect of AmB and Ag-NPs was found at the concentrations of 20 and 4 mg/ml, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. The mortality rate of protoscoleces in the negative control group was 7.1% after 60 min of exposure, while the scolicidal power of 10, 15 and 20% hypertonic saline as the positive controls was 100% after 15, 10 and 10 min of application (Fig. 6). Results also revealed that among tested drugs, the scolicidal effect of various concentrations of F. vulgare essential oil was extremely significant (P<0.05) compared to the control group at all exposure times.


Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces.

Lashkarizadeh MR, Asgaripour K, Saedi Dezaki E, Fasihi Harandi M - Iran J Parasitol (2015 Apr-Jun)

Scolicidal effects of hypertonic saline against protoscoleces of Echinococcus granulosus at various concentrations following different exposure times
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522296&req=5

Figure 6: Scolicidal effects of hypertonic saline against protoscoleces of Echinococcus granulosus at various concentrations following different exposure times
Mentions: The findings revealed that among tested drugs, F. vulgare essential oil and hypertonic saline had potent scolicidal effects against protoscoleces of hydatid cysts in a dose-dependent and also time-dependent manner, while AmB and Ag-NPs demonstrated weak scolocidal activities on protoscoleces of hydatid cysts (Fig. 3 and 4). F. vulgare essential oil at the concentration of 1 mg/ml after 5 min of exposure killed 100% protoscoleces. Similarly, the mean of mortality rate of protoscoleces after 10 min of exposure to concentration of 0.5 mg/ml was 100%. The scolicidal effect of F. vulgare essential oil at the concentration of 0.25 mg/ml was 11.6, 42.6, 73.3, 100 and 100% after 5, 10, 20, 30 and 60 min of incubation, respectively. These values for the concentration of 0.125 mg/ml were 5, 18.3, 39.6, 57.3 and 73.6% respectively (Fig. 5). In contrast, the maximum protoscolicidal effect of AmB and Ag-NPs was found at the concentrations of 20 and 4 mg/ml, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. The mortality rate of protoscoleces in the negative control group was 7.1% after 60 min of exposure, while the scolicidal power of 10, 15 and 20% hypertonic saline as the positive controls was 100% after 15, 10 and 10 min of application (Fig. 6). Results also revealed that among tested drugs, the scolicidal effect of various concentrations of F. vulgare essential oil was extremely significant (P<0.05) compared to the control group at all exposure times.

Bottom Line: Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively.In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT

Background: Surgery is the preferred treatment for hydatid cyst (cystic echinococcosis, CE). At present, various scolicidal agents have been used for inactivation of protoscoleces during surgery, but they are associated with adverse side effects. The aim of the present study was to evaluate the scolicidal effects of amphotricin B, Silver nano particles, Foeniculum vulgare Mill, essential oil and hypertonic saline against protoscoleces of hydatid cyst on an in vitro model.

Methods: Protoscoleces were aseptically aspirated from the naturally infected livers of sheep and goats. Various concentrations of AmB (2.5-20 mg/ml), Ag-NPs (0.5-4 mg/ml), F. vulgare essential oil (0.125-1 mg/ml) and hypertonic saline (10-20%) were used for 5-60 min. Eosin exclusion test was used to determine the viability of protoscoleces.

Results: Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.

Conclusion: The results indicated weak scolicidal activity of AmB and Ag-NPs; whereas F. vulgare essential oil had potent scolicidal activity against protoscoleces of hydatid cyst that revealed the potential of F. vulgare as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

No MeSH data available.


Related in: MedlinePlus