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Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization.

Meshgi B, Jalousian F, Masih Z - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.The genotypic results are in agreement with the phenotypic findings of both species.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).

ABSTRACT

Background: Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel.

Methods: The identification took place based on the morphometrics of the spicules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each animal) at the slaughterhouses from different localities in Iran. Samples were morphologically identified according to the spicules' morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.

Results: PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. contortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different fragments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.

Conclusion: The genotypic results are in agreement with the phenotypic findings of both species.

No MeSH data available.


Related in: MedlinePlus

Alignment of the ITS2 nuclear ribosomal DNA sequences (5′ to 3′) of H. contortus of sheep and H. longistipes of camel, by Clustal W2, Conserved nucleotides are indicated with asterisks and gaps are indicated with hyphens. AB682683.1 isolated from camel and HQ 844231.1 isolated from sheep
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Figure 4: Alignment of the ITS2 nuclear ribosomal DNA sequences (5′ to 3′) of H. contortus of sheep and H. longistipes of camel, by Clustal W2, Conserved nucleotides are indicated with asterisks and gaps are indicated with hyphens. AB682683.1 isolated from camel and HQ 844231.1 isolated from sheep

Mentions: Despite the fact that differences in 16 nucleotides between H. contortus and H. longistipes were observed, all the ITS2-rDNA complete sequence of H. contortus and H. longistipes showed to be 100% identical with the data sequence presented in the GenBank database (Fig. 4).


Phylogenetic Study of Haemonchus Species from Iran Based On Morpho-Molecular Characterization.

Meshgi B, Jalousian F, Masih Z - Iran J Parasitol (2015 Apr-Jun)

Alignment of the ITS2 nuclear ribosomal DNA sequences (5′ to 3′) of H. contortus of sheep and H. longistipes of camel, by Clustal W2, Conserved nucleotides are indicated with asterisks and gaps are indicated with hyphens. AB682683.1 isolated from camel and HQ 844231.1 isolated from sheep
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522294&req=5

Figure 4: Alignment of the ITS2 nuclear ribosomal DNA sequences (5′ to 3′) of H. contortus of sheep and H. longistipes of camel, by Clustal W2, Conserved nucleotides are indicated with asterisks and gaps are indicated with hyphens. AB682683.1 isolated from camel and HQ 844231.1 isolated from sheep
Mentions: Despite the fact that differences in 16 nucleotides between H. contortus and H. longistipes were observed, all the ITS2-rDNA complete sequence of H. contortus and H. longistipes showed to be 100% identical with the data sequence presented in the GenBank database (Fig. 4).

Bottom Line: A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.The genotypic results are in agreement with the phenotypic findings of both species.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes).

ABSTRACT

Background: Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel.

Methods: The identification took place based on the morphometrics of the spicules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each animal) at the slaughterhouses from different localities in Iran. Samples were morphologically identified according to the spicules' morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results.

Results: PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. contortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different fragments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively.

Conclusion: The genotypic results are in agreement with the phenotypic findings of both species.

No MeSH data available.


Related in: MedlinePlus