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Anti-Toxoplasma Activity of 2-(Naphthalene-2-γlthiol)-1H Indole.

Asgari Q, Keshavarz H, Rezaeian M, Sadeghpour H, Miri R, Motazedian MH - Iran J Parasitol (2015 Apr-Jun)

Bottom Line: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 μmol.Our findings demonstrated the toxoplasmocidal activity of the compound.The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1H-indole.

Methods: Tachyzoites of RH strain were incubated in various concentrations of 2-(naphthalene-2-ylthio)-1H-indole (25-800 μM) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS). To evaluate the infectivity, the tachyzoites exposed to the different concentrations of the compound were inoculated to 10 BALB/c mice groups. For Control, parasites exposed to DMSO (0.2% v/v) were also intraperitoneally inoculated into two groups of mice. The immunity of the exposed tachyzoites was evaluated by inoculation of the naïve parasite to the survived mice.

Results: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 μmol. The longevity of mice was dose dependent. Five mice out of group 400μmol and 3 out of group 800μmol showed immunization to the parasite.

Conclusion: Our findings demonstrated the toxoplasmocidal activity of the compound. The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

No MeSH data available.


Related in: MedlinePlus

Flowcytometry analyses of unexposed Toxoplasma tachyzoites and Toxoplasma tachyzoites exposed to saponine and different doses of 2-(naphthalene-2-ylthio)-1H-indole
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Figure 2: Flowcytometry analyses of unexposed Toxoplasma tachyzoites and Toxoplasma tachyzoites exposed to saponine and different doses of 2-(naphthalene-2-ylthio)-1H-indole

Mentions: According to the flowcytometry findings, approximately 85% of the Toxoplasma tachyzoites obtained from peritoneal passages could survive. Apoptosis or mortality was seen in 91% of tachyzoites that were exposed to 0.2% saponin and stained by PI (Fig.2). Flow cytometry analyses of different concentration (25–800 μM) of 2-(naphthalene-2-ylthio)-1H-indole on Toxoplasma tachyzoites viability was demonstrated in Fig. 3. The IC50 of the compound was 57 μM.


Anti-Toxoplasma Activity of 2-(Naphthalene-2-γlthiol)-1H Indole.

Asgari Q, Keshavarz H, Rezaeian M, Sadeghpour H, Miri R, Motazedian MH - Iran J Parasitol (2015 Apr-Jun)

Flowcytometry analyses of unexposed Toxoplasma tachyzoites and Toxoplasma tachyzoites exposed to saponine and different doses of 2-(naphthalene-2-ylthio)-1H-indole
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522292&req=5

Figure 2: Flowcytometry analyses of unexposed Toxoplasma tachyzoites and Toxoplasma tachyzoites exposed to saponine and different doses of 2-(naphthalene-2-ylthio)-1H-indole
Mentions: According to the flowcytometry findings, approximately 85% of the Toxoplasma tachyzoites obtained from peritoneal passages could survive. Apoptosis or mortality was seen in 91% of tachyzoites that were exposed to 0.2% saponin and stained by PI (Fig.2). Flow cytometry analyses of different concentration (25–800 μM) of 2-(naphthalene-2-ylthio)-1H-indole on Toxoplasma tachyzoites viability was demonstrated in Fig. 3. The IC50 of the compound was 57 μM.

Bottom Line: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 μmol.Our findings demonstrated the toxoplasmocidal activity of the compound.The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Background: This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1H-indole.

Methods: Tachyzoites of RH strain were incubated in various concentrations of 2-(naphthalene-2-ylthio)-1H-indole (25-800 μM) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS). To evaluate the infectivity, the tachyzoites exposed to the different concentrations of the compound were inoculated to 10 BALB/c mice groups. For Control, parasites exposed to DMSO (0.2% v/v) were also intraperitoneally inoculated into two groups of mice. The immunity of the exposed tachyzoites was evaluated by inoculation of the naïve parasite to the survived mice.

Results: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 μmol. The longevity of mice was dose dependent. Five mice out of group 400μmol and 3 out of group 800μmol showed immunization to the parasite.

Conclusion: Our findings demonstrated the toxoplasmocidal activity of the compound. The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines.

No MeSH data available.


Related in: MedlinePlus