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Production of poly(3-hydroxybutyrate) by Halomonas boliviensis in an air-lift reactor.

Rivera-Terceros P, Tito-Claros E, Torrico S, Carballo S, Van-Thuoc D, Quillaguamán J - J Biol Res (Thessalon) (2015)

Bottom Line: The largest amount of PHB, 41 % (wt.), was attained after 24 hrs of cultivation during which maltose in the hydrolysate was assimilated more rapidly than glucose during active cell growth; however, the rate of assimilation of both the carbohydrates was found to be similar during synthesis of PHB.Both maltose and glucose in the hydrolysate induce cell growth and PHB synthesis; most likely the cells balance adequately CoA and NAD(P)H during the assimilation of these carbohydrates.The combination of cheap substrates, simple production systems and the use of non-strict sterile conditions by the halophile H. boliviensis are desirable traits for large scale production of PHB, and should lead to a competitive bioprocess.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology, Faculty of Sciences and Technology, San Simon University, Cochabamba, Bolivia.

ABSTRACT

Background: Microbial polyesters, also known as polyhydroxyalkanoates (PHAs), closely resemble physical and mechanical features of petroleum derived plastics. Recombinant Escherichia coli strains are being used in industrial production of PHAs in Stirred Tank Bioreactors (STRs). However, use of Air-Lift Reactors (ALRs) has been known to offer numerous technical operating options over STRs, and as such has been successfully implemented in many bioprocesses. Halomonas boliviensis is a halophilic bacterium that is known to assimilate various carbohydrates and convert them into a particular type of PHA known as poly(3-hydroxybutyrate) (PHB). Owing to this capability, it has been used to synthesize the polyester using hydrolysates of starch or wheat bran in stirred tank bioreactors.

Results: This research article firstly describes the production of PHB in shake flasks by H. boliviensis using different combinations of carbohydrates and partially hydrolyzed starch as carbon sources. The highest PHB yields, between 56 and 61 % (wt.), were achieved when either starch hydrolysate or a mixture of glucose and xylose were used as carbon sources. The starch hydrolysate obtained in this study was then used as carbon source in an ALR. The largest amount of PHB, 41 % (wt.), was attained after 24 hrs of cultivation during which maltose in the hydrolysate was assimilated more rapidly than glucose during active cell growth; however, the rate of assimilation of both the carbohydrates was found to be similar during synthesis of PHB. An incomplete pentose phosphate pathway, which lacks 6-phosphogluconate dehydrogenase, was deduced from the genome sequence of this bacterium and may result in the characteristic assimilation of glucose and maltose by the cells.

Conclusions: This study showed that the production of PHB by H. boliviensis using cheap substrates such as starch hydrolysate in a simple production system involving an ALR is feasible. Both maltose and glucose in the hydrolysate induce cell growth and PHB synthesis; most likely the cells balance adequately CoA and NAD(P)H during the assimilation of these carbohydrates. The combination of cheap substrates, simple production systems and the use of non-strict sterile conditions by the halophile H. boliviensis are desirable traits for large scale production of PHB, and should lead to a competitive bioprocess.

No MeSH data available.


Related in: MedlinePlus

Cell growth, PHB production, and carbohydrate uptake by H. boliviensis as a function of production time in an ALR operated in a batch system. Air flow rate into the reactor was set at 2.0 L min−1, pH was maintained at 7.5 ± 0.3 and water at 35 °C was circulated by the jacket of the reactor. The initial MSG concentration was 2 g L−1. The experiments were performed in triplicate
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Fig2: Cell growth, PHB production, and carbohydrate uptake by H. boliviensis as a function of production time in an ALR operated in a batch system. Air flow rate into the reactor was set at 2.0 L min−1, pH was maintained at 7.5 ± 0.3 and water at 35 °C was circulated by the jacket of the reactor. The initial MSG concentration was 2 g L−1. The experiments were performed in triplicate

Mentions: Starch hydrolysate was used as carbon source in an ALR (Fig. 1). CDW, PHB accumulation, RCM, and glucose and maltose consumed by the bacterium as a function of production time are shown in Fig. 2. Glucose and maltose in the starch hydrolysate were rapidly assimilated and used mainly in the formation of cell biomass up to the first 12 hrs of cultivation; during this time period, H. boliviensis yielded only 10.5 % (wt.) PHB (Fig. 2), with maltose being assimilated faster than glucose. Between 12 and 24 hrs of cultivation, glucose and maltose were consumed at almost the same rate, with concomitant increase in PHB production that reached a maximum of 41 % (wt.) (Fig. 2). The onset of PHB depolymerization coincided with low concentrations of glucose and maltose in the medium. During this time, cells continued to grow as observed by the increase in RCM, although they were only using glucose as carbon source.Fig. 1


Production of poly(3-hydroxybutyrate) by Halomonas boliviensis in an air-lift reactor.

Rivera-Terceros P, Tito-Claros E, Torrico S, Carballo S, Van-Thuoc D, Quillaguamán J - J Biol Res (Thessalon) (2015)

Cell growth, PHB production, and carbohydrate uptake by H. boliviensis as a function of production time in an ALR operated in a batch system. Air flow rate into the reactor was set at 2.0 L min−1, pH was maintained at 7.5 ± 0.3 and water at 35 °C was circulated by the jacket of the reactor. The initial MSG concentration was 2 g L−1. The experiments were performed in triplicate
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4522284&req=5

Fig2: Cell growth, PHB production, and carbohydrate uptake by H. boliviensis as a function of production time in an ALR operated in a batch system. Air flow rate into the reactor was set at 2.0 L min−1, pH was maintained at 7.5 ± 0.3 and water at 35 °C was circulated by the jacket of the reactor. The initial MSG concentration was 2 g L−1. The experiments were performed in triplicate
Mentions: Starch hydrolysate was used as carbon source in an ALR (Fig. 1). CDW, PHB accumulation, RCM, and glucose and maltose consumed by the bacterium as a function of production time are shown in Fig. 2. Glucose and maltose in the starch hydrolysate were rapidly assimilated and used mainly in the formation of cell biomass up to the first 12 hrs of cultivation; during this time period, H. boliviensis yielded only 10.5 % (wt.) PHB (Fig. 2), with maltose being assimilated faster than glucose. Between 12 and 24 hrs of cultivation, glucose and maltose were consumed at almost the same rate, with concomitant increase in PHB production that reached a maximum of 41 % (wt.) (Fig. 2). The onset of PHB depolymerization coincided with low concentrations of glucose and maltose in the medium. During this time, cells continued to grow as observed by the increase in RCM, although they were only using glucose as carbon source.Fig. 1

Bottom Line: The largest amount of PHB, 41 % (wt.), was attained after 24 hrs of cultivation during which maltose in the hydrolysate was assimilated more rapidly than glucose during active cell growth; however, the rate of assimilation of both the carbohydrates was found to be similar during synthesis of PHB.Both maltose and glucose in the hydrolysate induce cell growth and PHB synthesis; most likely the cells balance adequately CoA and NAD(P)H during the assimilation of these carbohydrates.The combination of cheap substrates, simple production systems and the use of non-strict sterile conditions by the halophile H. boliviensis are desirable traits for large scale production of PHB, and should lead to a competitive bioprocess.

View Article: PubMed Central - PubMed

Affiliation: Center of Biotechnology, Faculty of Sciences and Technology, San Simon University, Cochabamba, Bolivia.

ABSTRACT

Background: Microbial polyesters, also known as polyhydroxyalkanoates (PHAs), closely resemble physical and mechanical features of petroleum derived plastics. Recombinant Escherichia coli strains are being used in industrial production of PHAs in Stirred Tank Bioreactors (STRs). However, use of Air-Lift Reactors (ALRs) has been known to offer numerous technical operating options over STRs, and as such has been successfully implemented in many bioprocesses. Halomonas boliviensis is a halophilic bacterium that is known to assimilate various carbohydrates and convert them into a particular type of PHA known as poly(3-hydroxybutyrate) (PHB). Owing to this capability, it has been used to synthesize the polyester using hydrolysates of starch or wheat bran in stirred tank bioreactors.

Results: This research article firstly describes the production of PHB in shake flasks by H. boliviensis using different combinations of carbohydrates and partially hydrolyzed starch as carbon sources. The highest PHB yields, between 56 and 61 % (wt.), were achieved when either starch hydrolysate or a mixture of glucose and xylose were used as carbon sources. The starch hydrolysate obtained in this study was then used as carbon source in an ALR. The largest amount of PHB, 41 % (wt.), was attained after 24 hrs of cultivation during which maltose in the hydrolysate was assimilated more rapidly than glucose during active cell growth; however, the rate of assimilation of both the carbohydrates was found to be similar during synthesis of PHB. An incomplete pentose phosphate pathway, which lacks 6-phosphogluconate dehydrogenase, was deduced from the genome sequence of this bacterium and may result in the characteristic assimilation of glucose and maltose by the cells.

Conclusions: This study showed that the production of PHB by H. boliviensis using cheap substrates such as starch hydrolysate in a simple production system involving an ALR is feasible. Both maltose and glucose in the hydrolysate induce cell growth and PHB synthesis; most likely the cells balance adequately CoA and NAD(P)H during the assimilation of these carbohydrates. The combination of cheap substrates, simple production systems and the use of non-strict sterile conditions by the halophile H. boliviensis are desirable traits for large scale production of PHB, and should lead to a competitive bioprocess.

No MeSH data available.


Related in: MedlinePlus