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Expression of AKR1C3 and CNN3 as markers for detection of lymph node metastases in colorectal cancer.

Nakarai C, Osawa K, Akiyama M, Matsubara N, Ikeuchi H, Yamano T, Hirota S, Tomita N, Usami M, Kido Y - Clin. Exp. Med. (2014)

Bottom Line: The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo-keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing].These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients.There were significant correlations between the expression levels of AKR1C3 and CNN3.

View Article: PubMed Central - PubMed

Affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe, 654-0142, Japan.

ABSTRACT
The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo-keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing]. These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients. The level of AKR1C3 mRNA expression was significantly different between the Dukes' stage A, B, and C groups and the control group (p < 0.05, p < 0.001, and p < 0.001) and was also significantly different between Dukes' stage C and A or B groups (p < 0.05 and p < 0.001, respectively). The expression of CNN3 was significantly different between the Dukes' stage C and B or control groups (p < 0.001 and p < 0.01, respectively). There were significant correlations between the expression levels of AKR1C3 and CNN3. AKR1C3 and CNN3 expressions are more accurate and suitable markers for the diagnosis of LN metastasis than the other six genes examined in this study.

No MeSH data available.


Related in: MedlinePlus

Relative mRNA expression of S100P, AKR1C3, CNN3, AKR1B1, MACC1, HBE1, TFF3, and FGGY in tissues from colorectal cancer (CRC) patients determined by real-time quantitative RT-PCR. Dots showed mRNA levels in 13 non-tumor tissues and 14 tumor tissues from CRC patients compared with 11 inflammatory tissues from ulcerative colitis patients as controls. Bars showed means. a The relative quantity values (Mean ± SD) of S100P are 28.68 ± 24.20, 14.43 ± 12.90, and 199.51 ± 549.78 (control, non-tumor, and tumor). b Those of AKR1C3 are 21.09 ± 26.93, 214.87 ± 291.55, and 124.88 ± 266.74. c Those of CNN3 are 2.86 ± 1.53, 10.44 ± 16.20, and 6.93 ± 10.30. d Those of AKR1B1 are 1.32 ± 1.57, 2.15 ± 3.55, and 0.96 ± 1.63. e Those of MACC1 are 10.04 ± 6.12, 69.13 ± 158.41, and 145.37 ± 289.74. f Those of HBE are 0.89 ± 1.43, 0.55 ± 1.15, and 0.67 ± 1.61. g Those of TFF3 are 154.79 ± 216.76, 1,116.46 ± 1,610.72, and 908.91 ± 1,158.59. h Those of FGGY are 1.02 ± 0.50, 13.30 ± 25.12, and 30.65 ± 49.14, respectively. The p values are based on Kruskal–Wallis test. +p<0.05 and *p < 0.01 are based on Mann–Whitney U test with a Bonferroni correction
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Fig1: Relative mRNA expression of S100P, AKR1C3, CNN3, AKR1B1, MACC1, HBE1, TFF3, and FGGY in tissues from colorectal cancer (CRC) patients determined by real-time quantitative RT-PCR. Dots showed mRNA levels in 13 non-tumor tissues and 14 tumor tissues from CRC patients compared with 11 inflammatory tissues from ulcerative colitis patients as controls. Bars showed means. a The relative quantity values (Mean ± SD) of S100P are 28.68 ± 24.20, 14.43 ± 12.90, and 199.51 ± 549.78 (control, non-tumor, and tumor). b Those of AKR1C3 are 21.09 ± 26.93, 214.87 ± 291.55, and 124.88 ± 266.74. c Those of CNN3 are 2.86 ± 1.53, 10.44 ± 16.20, and 6.93 ± 10.30. d Those of AKR1B1 are 1.32 ± 1.57, 2.15 ± 3.55, and 0.96 ± 1.63. e Those of MACC1 are 10.04 ± 6.12, 69.13 ± 158.41, and 145.37 ± 289.74. f Those of HBE are 0.89 ± 1.43, 0.55 ± 1.15, and 0.67 ± 1.61. g Those of TFF3 are 154.79 ± 216.76, 1,116.46 ± 1,610.72, and 908.91 ± 1,158.59. h Those of FGGY are 1.02 ± 0.50, 13.30 ± 25.12, and 30.65 ± 49.14, respectively. The p values are based on Kruskal–Wallis test. +p<0.05 and *p < 0.01 are based on Mann–Whitney U test with a Bonferroni correction

Mentions: qRT-PCR was performed to quantify these genes in tumor tissues (n = 14), non-tumor tissues from CRC patients (n = 13), and inflammatory tissues from patients with UC; the latter tissues served as the controls (n = 11). The results are shown in Fig. 1. There were no significant differences in the relative levels of mRNA expression for S100P, AKR1C3, CNN3, AKR1B1, HBE1, and TFF3 among tumor tissues, non-tumor tissues, and inflammatory tissues. For MACC1, there were significant differences in levels of expression between tumor tissues (Mean ± SD, 145.37 ± 289.74), non-tumor tissues (Mean ± SD, 69.13 ± 158.41), and inflammatory tissues (Mean ± SD, 10.04 ± 6.12) (Kruskal–Wallis test; p < 0.05). MACC1 mRNA expression was significantly different between tumor and inflammatory tissues (Mann–Whitney U test with a Bonferroni correction; p < 0.05). For FGGY, there were significant differences in levels of expression between tumor tissues (Mean ± SD, 30.65 ± 49.14), non-tumor tissues (Mean ± SD, 13.30 ± 25.12), and inflammatory tissues (Mean ± SD, 1.02 ± 0.50) (Kruskal–Wallis test; p < 0.001). A subsequent Mann–Whitney U test with a Bonferroni correction showed that the mean values for FGGY expression were significantly different between non-tumor and inflammatory tissues (p < 0.05) and between tumor and inflammatory tissues (p < 0.01).Fig. 1


Expression of AKR1C3 and CNN3 as markers for detection of lymph node metastases in colorectal cancer.

Nakarai C, Osawa K, Akiyama M, Matsubara N, Ikeuchi H, Yamano T, Hirota S, Tomita N, Usami M, Kido Y - Clin. Exp. Med. (2014)

Relative mRNA expression of S100P, AKR1C3, CNN3, AKR1B1, MACC1, HBE1, TFF3, and FGGY in tissues from colorectal cancer (CRC) patients determined by real-time quantitative RT-PCR. Dots showed mRNA levels in 13 non-tumor tissues and 14 tumor tissues from CRC patients compared with 11 inflammatory tissues from ulcerative colitis patients as controls. Bars showed means. a The relative quantity values (Mean ± SD) of S100P are 28.68 ± 24.20, 14.43 ± 12.90, and 199.51 ± 549.78 (control, non-tumor, and tumor). b Those of AKR1C3 are 21.09 ± 26.93, 214.87 ± 291.55, and 124.88 ± 266.74. c Those of CNN3 are 2.86 ± 1.53, 10.44 ± 16.20, and 6.93 ± 10.30. d Those of AKR1B1 are 1.32 ± 1.57, 2.15 ± 3.55, and 0.96 ± 1.63. e Those of MACC1 are 10.04 ± 6.12, 69.13 ± 158.41, and 145.37 ± 289.74. f Those of HBE are 0.89 ± 1.43, 0.55 ± 1.15, and 0.67 ± 1.61. g Those of TFF3 are 154.79 ± 216.76, 1,116.46 ± 1,610.72, and 908.91 ± 1,158.59. h Those of FGGY are 1.02 ± 0.50, 13.30 ± 25.12, and 30.65 ± 49.14, respectively. The p values are based on Kruskal–Wallis test. +p<0.05 and *p < 0.01 are based on Mann–Whitney U test with a Bonferroni correction
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Fig1: Relative mRNA expression of S100P, AKR1C3, CNN3, AKR1B1, MACC1, HBE1, TFF3, and FGGY in tissues from colorectal cancer (CRC) patients determined by real-time quantitative RT-PCR. Dots showed mRNA levels in 13 non-tumor tissues and 14 tumor tissues from CRC patients compared with 11 inflammatory tissues from ulcerative colitis patients as controls. Bars showed means. a The relative quantity values (Mean ± SD) of S100P are 28.68 ± 24.20, 14.43 ± 12.90, and 199.51 ± 549.78 (control, non-tumor, and tumor). b Those of AKR1C3 are 21.09 ± 26.93, 214.87 ± 291.55, and 124.88 ± 266.74. c Those of CNN3 are 2.86 ± 1.53, 10.44 ± 16.20, and 6.93 ± 10.30. d Those of AKR1B1 are 1.32 ± 1.57, 2.15 ± 3.55, and 0.96 ± 1.63. e Those of MACC1 are 10.04 ± 6.12, 69.13 ± 158.41, and 145.37 ± 289.74. f Those of HBE are 0.89 ± 1.43, 0.55 ± 1.15, and 0.67 ± 1.61. g Those of TFF3 are 154.79 ± 216.76, 1,116.46 ± 1,610.72, and 908.91 ± 1,158.59. h Those of FGGY are 1.02 ± 0.50, 13.30 ± 25.12, and 30.65 ± 49.14, respectively. The p values are based on Kruskal–Wallis test. +p<0.05 and *p < 0.01 are based on Mann–Whitney U test with a Bonferroni correction
Mentions: qRT-PCR was performed to quantify these genes in tumor tissues (n = 14), non-tumor tissues from CRC patients (n = 13), and inflammatory tissues from patients with UC; the latter tissues served as the controls (n = 11). The results are shown in Fig. 1. There were no significant differences in the relative levels of mRNA expression for S100P, AKR1C3, CNN3, AKR1B1, HBE1, and TFF3 among tumor tissues, non-tumor tissues, and inflammatory tissues. For MACC1, there were significant differences in levels of expression between tumor tissues (Mean ± SD, 145.37 ± 289.74), non-tumor tissues (Mean ± SD, 69.13 ± 158.41), and inflammatory tissues (Mean ± SD, 10.04 ± 6.12) (Kruskal–Wallis test; p < 0.05). MACC1 mRNA expression was significantly different between tumor and inflammatory tissues (Mann–Whitney U test with a Bonferroni correction; p < 0.05). For FGGY, there were significant differences in levels of expression between tumor tissues (Mean ± SD, 30.65 ± 49.14), non-tumor tissues (Mean ± SD, 13.30 ± 25.12), and inflammatory tissues (Mean ± SD, 1.02 ± 0.50) (Kruskal–Wallis test; p < 0.001). A subsequent Mann–Whitney U test with a Bonferroni correction showed that the mean values for FGGY expression were significantly different between non-tumor and inflammatory tissues (p < 0.05) and between tumor and inflammatory tissues (p < 0.01).Fig. 1

Bottom Line: The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo-keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing].These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients.There were significant correlations between the expression levels of AKR1C3 and CNN3.

View Article: PubMed Central - PubMed

Affiliation: Department of Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe, 654-0142, Japan.

ABSTRACT
The aim of the study was to identify a set of discriminating genes that could be used for the prediction of Lymph node (LN) metastasis in human colorectal cancer (CRC), and for this, we compared the whole genome profiles of two CRC cell lines (the primary cell line SW480 and its LN metastatic variant, SW620) and identified eight genes [S100 calcium-binding protein P; aldo-keto reductase family 1(AKR1), member B1 (aldose reductase; AKR1B1); AKR1, member C3 (AKR1C3); calponin 3, acidic; metastasis associated in colon cancer 1; hemoglobin, epsilon 1; trefoil factor 3; and FGGY carbohydrate kinase domain containing]. These genes were examined by quantitative RT-PCR in tissues and LNs in 14 CRC patients and 11 control patients. The level of AKR1C3 mRNA expression was significantly different between the Dukes' stage A, B, and C groups and the control group (p < 0.05, p < 0.001, and p < 0.001) and was also significantly different between Dukes' stage C and A or B groups (p < 0.05 and p < 0.001, respectively). The expression of CNN3 was significantly different between the Dukes' stage C and B or control groups (p < 0.001 and p < 0.01, respectively). There were significant correlations between the expression levels of AKR1C3 and CNN3. AKR1C3 and CNN3 expressions are more accurate and suitable markers for the diagnosis of LN metastasis than the other six genes examined in this study.

No MeSH data available.


Related in: MedlinePlus