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Hesperetin prevents selenite-induced cataract in rats.

Nakazawa Y, Oka M, Bando M, Takehana M - Mol. Vis. (2015)

Bottom Line: Lenses were observed with slit-lamp microscopy, and filensin degradation and the decreased glutathione (GSH) and ascorbic acid levels in the lens were measured on day 6.Expression of the 94 kDa and 50 kDa forms of filensin was significantly decreased in the lenses in the G3 group compared with those in the G1 and G2 groups.In the G3 group lenses, the GSH and ascorbic acid levels were lower than in the control group but were normalized in the G4 group lenses.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Function and Physiology, Faculty of Pharmacy, Keio University ; Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University.

ABSTRACT

Purpose: This study investigated the ability of hesperetin, a natural flavonoid, to prevent selenite-induced cataracts in a rat model.

Methods: Animals were divided into four treatment groups: G1 (control group), G2 (hesperetin-treated group), G3 (selenite-induced cataract group), and G4 (hesperetin-treated selenite cataract group). Animals in the G1 and G3 groups were injected with vehicle alone, while those in the G2 and G4 groups received a subcutaneous injection of hesperetin (0.4 μg/g bodyweight on days 0, 1, and 2, corresponding to P13, P14, and P15). Sodium selenite (20 μmol/g bodyweight given 4 h after the hesperetin injection on day 0) was administered to rats in the G3 and G4 groups to induce cataract formation. Lenses were observed with slit-lamp microscopy, and filensin degradation and the decreased glutathione (GSH) and ascorbic acid levels in the lens were measured on day 6.

Results: Lenses in the G3 group showed mature central opacity, while some lenses in the G4 group lacked central opacity and had lower-grade cataracts. All lenses in the G1 and G2 groups were transparent. Expression of the 94 kDa and 50 kDa forms of filensin was significantly decreased in the lenses in the G3 group compared with those in the G1 and G2 groups. Interestingly, these forms of filensin rescued the rat lenses in the G4 group. In the G3 group lenses, the GSH and ascorbic acid levels were lower than in the control group but were normalized in the G4 group lenses.

Conclusions: The results suggest that hesperetin can prevent selenite-induced cataract formation.

No MeSH data available.


Related in: MedlinePlus

AsA concentrations in lenses with selenite-induced cataracts. Group 1: control group. Group 2: hesperetin-treated rats. Group 3: selenite-treated rats. Group 4: rats cotreated with selenite and hesperetin. All results are expressed as the mean ± SEM, and statistical analysis was determined using the unpaired Student t-test. *p<0.05 versus control (Group 1) and †p<0.05 versus rats with selenite-induced cataracts without hesperetin treatment (Group 3; m = 9 in each group).
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f4: AsA concentrations in lenses with selenite-induced cataracts. Group 1: control group. Group 2: hesperetin-treated rats. Group 3: selenite-treated rats. Group 4: rats cotreated with selenite and hesperetin. All results are expressed as the mean ± SEM, and statistical analysis was determined using the unpaired Student t-test. *p<0.05 versus control (Group 1) and †p<0.05 versus rats with selenite-induced cataracts without hesperetin treatment (Group 3; m = 9 in each group).

Mentions: The AsA concentration was 30.0 μg/g wet lens weight in the G1 group, similar to that in the G2 group (28.9 μg/g). The AsA concentration was significantly lower in the G3 group than in the control group (17.2 μg/g), which was 57.3% concentration compared to the G1 group. The cotreatment with hesperetin and selenite (the G4 group) rescued the decreasing AsA concentration in the selenite-induced rat cataracts (25.0 μg/g), which was 83.3% concentration compared to the G1 group (Figure 4).


Hesperetin prevents selenite-induced cataract in rats.

Nakazawa Y, Oka M, Bando M, Takehana M - Mol. Vis. (2015)

AsA concentrations in lenses with selenite-induced cataracts. Group 1: control group. Group 2: hesperetin-treated rats. Group 3: selenite-treated rats. Group 4: rats cotreated with selenite and hesperetin. All results are expressed as the mean ± SEM, and statistical analysis was determined using the unpaired Student t-test. *p<0.05 versus control (Group 1) and †p<0.05 versus rats with selenite-induced cataracts without hesperetin treatment (Group 3; m = 9 in each group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522242&req=5

f4: AsA concentrations in lenses with selenite-induced cataracts. Group 1: control group. Group 2: hesperetin-treated rats. Group 3: selenite-treated rats. Group 4: rats cotreated with selenite and hesperetin. All results are expressed as the mean ± SEM, and statistical analysis was determined using the unpaired Student t-test. *p<0.05 versus control (Group 1) and †p<0.05 versus rats with selenite-induced cataracts without hesperetin treatment (Group 3; m = 9 in each group).
Mentions: The AsA concentration was 30.0 μg/g wet lens weight in the G1 group, similar to that in the G2 group (28.9 μg/g). The AsA concentration was significantly lower in the G3 group than in the control group (17.2 μg/g), which was 57.3% concentration compared to the G1 group. The cotreatment with hesperetin and selenite (the G4 group) rescued the decreasing AsA concentration in the selenite-induced rat cataracts (25.0 μg/g), which was 83.3% concentration compared to the G1 group (Figure 4).

Bottom Line: Lenses were observed with slit-lamp microscopy, and filensin degradation and the decreased glutathione (GSH) and ascorbic acid levels in the lens were measured on day 6.Expression of the 94 kDa and 50 kDa forms of filensin was significantly decreased in the lenses in the G3 group compared with those in the G1 and G2 groups.In the G3 group lenses, the GSH and ascorbic acid levels were lower than in the control group but were normalized in the G4 group lenses.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Function and Physiology, Faculty of Pharmacy, Keio University ; Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University.

ABSTRACT

Purpose: This study investigated the ability of hesperetin, a natural flavonoid, to prevent selenite-induced cataracts in a rat model.

Methods: Animals were divided into four treatment groups: G1 (control group), G2 (hesperetin-treated group), G3 (selenite-induced cataract group), and G4 (hesperetin-treated selenite cataract group). Animals in the G1 and G3 groups were injected with vehicle alone, while those in the G2 and G4 groups received a subcutaneous injection of hesperetin (0.4 μg/g bodyweight on days 0, 1, and 2, corresponding to P13, P14, and P15). Sodium selenite (20 μmol/g bodyweight given 4 h after the hesperetin injection on day 0) was administered to rats in the G3 and G4 groups to induce cataract formation. Lenses were observed with slit-lamp microscopy, and filensin degradation and the decreased glutathione (GSH) and ascorbic acid levels in the lens were measured on day 6.

Results: Lenses in the G3 group showed mature central opacity, while some lenses in the G4 group lacked central opacity and had lower-grade cataracts. All lenses in the G1 and G2 groups were transparent. Expression of the 94 kDa and 50 kDa forms of filensin was significantly decreased in the lenses in the G3 group compared with those in the G1 and G2 groups. Interestingly, these forms of filensin rescued the rat lenses in the G4 group. In the G3 group lenses, the GSH and ascorbic acid levels were lower than in the control group but were normalized in the G4 group lenses.

Conclusions: The results suggest that hesperetin can prevent selenite-induced cataract formation.

No MeSH data available.


Related in: MedlinePlus