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A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging.

Jiang Y, Sun LL, Ren GZ, Niu X, Hu WZ, Hu ZQ - ChemistryOpen (2015)

Bottom Line: Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity.Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe.The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.

ABSTRACT
The development of novel selective probes with high sensitivity for the detection of Al(3+) is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, N'-(4-diethylamino-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (1), for Al(3+). Probe 1 was evaluated in a solution of acetonitrile/water (1:1 v/v). Compared with previously reported probes for Al(3+), probe 1 can be synthesized easily and in high yield. A Job plot confirmed that probe 1 is able to complex Al(3+) in a 1:1 ratio, and the binding constant was determined to be 4.25×10(8) m(-1). Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity. Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe 1 to act as a fluorescent probe for Al(3+) in living systems was evaluated in Gram-negative bacteria, Escherichia coli, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

No MeSH data available.


Related in: MedlinePlus

Effect of pH on the fluorescence intensity of chemosensor 1 (5 μm) (▪) at 467 nm, and 1 upon addition of 10 equiv Al3+ in CH3CN/H2O (1:1 v/v) (•).
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fig07: Effect of pH on the fluorescence intensity of chemosensor 1 (5 μm) (▪) at 467 nm, and 1 upon addition of 10 equiv Al3+ in CH3CN/H2O (1:1 v/v) (•).

Mentions: The pH influence on the sensitivity of probe 1 for Al3+ was also examined under various pH conditions (Figure 7). As expected, 1 was found to be weakly fluorescent in acetonitrile/water (1:1 v/v) and showed a significantly positive response (strong fluorescence) between pH 6.8 and 7.4 upon the addition of Al3+. The biologically relevant pH range is 6.0–7.6,20 thus, fluorescent probe 1 could be used to detect intracellular Al3+ without being affected by the physiological pH.


A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging.

Jiang Y, Sun LL, Ren GZ, Niu X, Hu WZ, Hu ZQ - ChemistryOpen (2015)

Effect of pH on the fluorescence intensity of chemosensor 1 (5 μm) (▪) at 467 nm, and 1 upon addition of 10 equiv Al3+ in CH3CN/H2O (1:1 v/v) (•).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522188&req=5

fig07: Effect of pH on the fluorescence intensity of chemosensor 1 (5 μm) (▪) at 467 nm, and 1 upon addition of 10 equiv Al3+ in CH3CN/H2O (1:1 v/v) (•).
Mentions: The pH influence on the sensitivity of probe 1 for Al3+ was also examined under various pH conditions (Figure 7). As expected, 1 was found to be weakly fluorescent in acetonitrile/water (1:1 v/v) and showed a significantly positive response (strong fluorescence) between pH 6.8 and 7.4 upon the addition of Al3+. The biologically relevant pH range is 6.0–7.6,20 thus, fluorescent probe 1 could be used to detect intracellular Al3+ without being affected by the physiological pH.

Bottom Line: Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity.Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe.The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.

ABSTRACT
The development of novel selective probes with high sensitivity for the detection of Al(3+) is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, N'-(4-diethylamino-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (1), for Al(3+). Probe 1 was evaluated in a solution of acetonitrile/water (1:1 v/v). Compared with previously reported probes for Al(3+), probe 1 can be synthesized easily and in high yield. A Job plot confirmed that probe 1 is able to complex Al(3+) in a 1:1 ratio, and the binding constant was determined to be 4.25×10(8) m(-1). Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity. Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe 1 to act as a fluorescent probe for Al(3+) in living systems was evaluated in Gram-negative bacteria, Escherichia coli, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

No MeSH data available.


Related in: MedlinePlus