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A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging.

Jiang Y, Sun LL, Ren GZ, Niu X, Hu WZ, Hu ZQ - ChemistryOpen (2015)

Bottom Line: Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity.Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe.The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.

ABSTRACT
The development of novel selective probes with high sensitivity for the detection of Al(3+) is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, N'-(4-diethylamino-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (1), for Al(3+). Probe 1 was evaluated in a solution of acetonitrile/water (1:1 v/v). Compared with previously reported probes for Al(3+), probe 1 can be synthesized easily and in high yield. A Job plot confirmed that probe 1 is able to complex Al(3+) in a 1:1 ratio, and the binding constant was determined to be 4.25×10(8) m(-1). Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity. Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe 1 to act as a fluorescent probe for Al(3+) in living systems was evaluated in Gram-negative bacteria, Escherichia coli, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

No MeSH data available.


Related in: MedlinePlus

a) UV-vis absorption spectra of 1 upon addition of various metal ions (5.0 equiv) in CH3CN/H2O (1:1 v/v); b) Absorption titration spectra of 1 (5 μm) in CH3CN/H2O (1:1 v/v) in the presence of increasing concentrations of Al3+ (0–8 equiv).
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fig03: a) UV-vis absorption spectra of 1 upon addition of various metal ions (5.0 equiv) in CH3CN/H2O (1:1 v/v); b) Absorption titration spectra of 1 (5 μm) in CH3CN/H2O (1:1 v/v) in the presence of increasing concentrations of Al3+ (0–8 equiv).

Mentions: The UV-vis absorption of 1 in acetonitrile/water (1:1 v/v) was investigated by spectrophotometric titration. As shown in Figure 3 a, in the presence of Al3+ (5.0 equiv), a new absorbance band appears at approximately 350 nm. However, no obvious changes in the UV-vis absorption spectra of 1 were observed in the presence of other metal ions. Furthermore, the absorbance peak at ∼350 nm increased gradually upon gradual addition of Al3+ to the solution of 1 (Figure 3 b).


A New Fluorescence Turn-On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging.

Jiang Y, Sun LL, Ren GZ, Niu X, Hu WZ, Hu ZQ - ChemistryOpen (2015)

a) UV-vis absorption spectra of 1 upon addition of various metal ions (5.0 equiv) in CH3CN/H2O (1:1 v/v); b) Absorption titration spectra of 1 (5 μm) in CH3CN/H2O (1:1 v/v) in the presence of increasing concentrations of Al3+ (0–8 equiv).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522188&req=5

fig03: a) UV-vis absorption spectra of 1 upon addition of various metal ions (5.0 equiv) in CH3CN/H2O (1:1 v/v); b) Absorption titration spectra of 1 (5 μm) in CH3CN/H2O (1:1 v/v) in the presence of increasing concentrations of Al3+ (0–8 equiv).
Mentions: The UV-vis absorption of 1 in acetonitrile/water (1:1 v/v) was investigated by spectrophotometric titration. As shown in Figure 3 a, in the presence of Al3+ (5.0 equiv), a new absorbance band appears at approximately 350 nm. However, no obvious changes in the UV-vis absorption spectra of 1 were observed in the presence of other metal ions. Furthermore, the absorbance peak at ∼350 nm increased gradually upon gradual addition of Al3+ to the solution of 1 (Figure 3 b).

Bottom Line: Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity.Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe.The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology Zhengzhou Road No. 53, Qingdao, 266042, P. R. China.

ABSTRACT
The development of novel selective probes with high sensitivity for the detection of Al(3+) is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, N'-(4-diethylamino-2-hydroxybenzylidene)-2-hydroxybenzohydrazide (1), for Al(3+). Probe 1 was evaluated in a solution of acetonitrile/water (1:1 v/v). Compared with previously reported probes for Al(3+), probe 1 can be synthesized easily and in high yield. A Job plot confirmed that probe 1 is able to complex Al(3+) in a 1:1 ratio, and the binding constant was determined to be 4.25×10(8) m(-1). Moreover, the detection limit was as low as 6.7×10(-9) m, suggesting that probe 1 has a high sensitivity. Common coexistent metal ions, such as K(+), Co(2+), Ca(2+), Ba(2+), Ni(2+), Pb(2+), Hg(2+), Ce(2+), Zn(2+), Cd(2+), Fe(3+), showed little or no interference in the detection of Al(3+) in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe 1 to act as a fluorescent probe for Al(3+) in living systems was evaluated in Gram-negative bacteria, Escherichia coli, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that 1 has appropriate properties to be developed for application as a fluorescent probe of Al(3+) for use in biological studies.

No MeSH data available.


Related in: MedlinePlus