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Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Related in: MedlinePlus

Effects of exposure to 460 nm light on calcium handling and contractile function in myocytes stored in 3 μmol L−1 blebbistatin.
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fig06: Effects of exposure to 460 nm light on calcium handling and contractile function in myocytes stored in 3 μmol L−1 blebbistatin.

Mentions: Prior experiments have shown that blebbistatin can be photoinactivated by blue light (Sakamoto et al. 2005; Kabaeva et al. 2008). We studied 12 myocytes that had previously been stored in blebbistatin for 2 days before and after exposure to blue light to test whether light could be used to reverse the contractile inhibition. As shown in Fig.6, 60 sec of exposure to 460 nm light partially reversed some of the effects of blebbistatin on cell shortening and calcium.


Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Effects of exposure to 460 nm light on calcium handling and contractile function in myocytes stored in 3 μmol L−1 blebbistatin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522161&req=5

fig06: Effects of exposure to 460 nm light on calcium handling and contractile function in myocytes stored in 3 μmol L−1 blebbistatin.
Mentions: Prior experiments have shown that blebbistatin can be photoinactivated by blue light (Sakamoto et al. 2005; Kabaeva et al. 2008). We studied 12 myocytes that had previously been stored in blebbistatin for 2 days before and after exposure to blue light to test whether light could be used to reverse the contractile inhibition. As shown in Fig.6, 60 sec of exposure to 460 nm light partially reversed some of the effects of blebbistatin on cell shortening and calcium.

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Related in: MedlinePlus