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Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Effects of storage in 3 μmol L−1 blebbistatin on calcium handling and contractile function.
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fig05: Effects of storage in 3 μmol L−1 blebbistatin on calcium handling and contractile function.

Mentions: Storing cells in 3 μmol L−1 blebbistatin was less effective at maintaining myocyte function than storing cells in BDM. As shown in Figure5, calcium transients were statistically different from control values after just 24 h of storage. The amplitude of shortening was also significantly reduced at this time-point and continued to decline in subsequent days.


Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Effects of storage in 3 μmol L−1 blebbistatin on calcium handling and contractile function.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522161&req=5

fig05: Effects of storage in 3 μmol L−1 blebbistatin on calcium handling and contractile function.
Mentions: Storing cells in 3 μmol L−1 blebbistatin was less effective at maintaining myocyte function than storing cells in BDM. As shown in Figure5, calcium transients were statistically different from control values after just 24 h of storage. The amplitude of shortening was also significantly reduced at this time-point and continued to decline in subsequent days.

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.