Limits...
Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Rod-shaped cell counts plotted by days of storage for each of the three storage conditions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4522161&req=5

fig03: Rod-shaped cell counts plotted by days of storage for each of the three storage conditions.

Mentions: Cells that were stored at 4°C without myosin ATPase inhibitors showed characteristic blebbing at their edges and had resting sarcomere lengths below 1.7 μm after 24 h. The normalized fraction of rod-shaped cells was 0.16 ± 0.08 after 24 h (Fig.3; Supplemental Fig. S1 and S2) and zero within 3 days.


Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Rod-shaped cell counts plotted by days of storage for each of the three storage conditions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522161&req=5

fig03: Rod-shaped cell counts plotted by days of storage for each of the three storage conditions.
Mentions: Cells that were stored at 4°C without myosin ATPase inhibitors showed characteristic blebbing at their edges and had resting sarcomere lengths below 1.7 μm after 24 h. The normalized fraction of rod-shaped cells was 0.16 ± 0.08 after 24 h (Fig.3; Supplemental Fig. S1 and S2) and zero within 3 days.

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.