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Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Cell morphology and sarcomere lengths. Top: Cells stored in perfusion solution (PS) show short sarcomere lengths (SL) and blebbing (arrows) after 1 day of storage at 4°C. Middle: Cells stored in PS + 10 mmol L−1 BDM maintain control sarcomere lengths for 2 days but begin to show blebbing. Cells shorten significantly after 3 days of storage. Bottom: Cells stored in PS + 3 μmol L−1 blebbistatin maintain control sarcomere lengths and do not exhibit blebbing.
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fig02: Cell morphology and sarcomere lengths. Top: Cells stored in perfusion solution (PS) show short sarcomere lengths (SL) and blebbing (arrows) after 1 day of storage at 4°C. Middle: Cells stored in PS + 10 mmol L−1 BDM maintain control sarcomere lengths for 2 days but begin to show blebbing. Cells shorten significantly after 3 days of storage. Bottom: Cells stored in PS + 3 μmol L−1 blebbistatin maintain control sarcomere lengths and do not exhibit blebbing.

Mentions: Freshly isolated myocytes that were tolerant to calcium had well-defined edges, clear striation patterns, and a distinctive rod-like shape (length to width ratio more than 2.5) (Fig.2).


Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Cell morphology and sarcomere lengths. Top: Cells stored in perfusion solution (PS) show short sarcomere lengths (SL) and blebbing (arrows) after 1 day of storage at 4°C. Middle: Cells stored in PS + 10 mmol L−1 BDM maintain control sarcomere lengths for 2 days but begin to show blebbing. Cells shorten significantly after 3 days of storage. Bottom: Cells stored in PS + 3 μmol L−1 blebbistatin maintain control sarcomere lengths and do not exhibit blebbing.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522161&req=5

fig02: Cell morphology and sarcomere lengths. Top: Cells stored in perfusion solution (PS) show short sarcomere lengths (SL) and blebbing (arrows) after 1 day of storage at 4°C. Middle: Cells stored in PS + 10 mmol L−1 BDM maintain control sarcomere lengths for 2 days but begin to show blebbing. Cells shorten significantly after 3 days of storage. Bottom: Cells stored in PS + 3 μmol L−1 blebbistatin maintain control sarcomere lengths and do not exhibit blebbing.
Mentions: Freshly isolated myocytes that were tolerant to calcium had well-defined edges, clear striation patterns, and a distinctive rod-like shape (length to width ratio more than 2.5) (Fig.2).

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.