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Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.


Parameters reported in this study.
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fig01: Parameters reported in this study.

Mentions: Calcium transients and unloaded shortening profiles were analyzed offline using custom-written software developed in MATLAB. Calcium fluorescence ratios were calculated by dividing the emission (510 nm) intensity values recorded during successive excitations by 340 nm and 380 nm wavelengths. Ratios and sarcomere length transients were filtered using Savitzky–Golay filters. The parameters that are reported here for calcium transients are as follows: Fura signal amplitude, time to peak, time to 50% relaxation, and tau (time constant of exponential decay) (Fig.1). The parameters that are reported for sarcomere length profiles are as follows: diastolic length, shortening amplitude, time to peak shortening, and time to 50% relaxation time.


Myocyte contractility can be maintained by storing cells with the myosin ATPase inhibitor 2,3 butanedione monoxime.

Chung CS, Mechas C, Campbell KS - Physiol Rep (2015)

Parameters reported in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4522161&req=5

fig01: Parameters reported in this study.
Mentions: Calcium transients and unloaded shortening profiles were analyzed offline using custom-written software developed in MATLAB. Calcium fluorescence ratios were calculated by dividing the emission (510 nm) intensity values recorded during successive excitations by 340 nm and 380 nm wavelengths. Ratios and sarcomere length transients were filtered using Savitzky–Golay filters. The parameters that are reported here for calcium transients are as follows: Fura signal amplitude, time to peak, time to 50% relaxation, and tau (time constant of exponential decay) (Fig.1). The parameters that are reported for sarcomere length profiles are as follows: diastolic length, shortening amplitude, time to peak shortening, and time to 50% relaxation time.

Bottom Line: Storage in blebbistatin maintained cell morphology for 72 h but inhibited contractility.These data show that storing cells with myosin ATPase inhibitors can extend the viability of myocytes that will be used for functional assays.This may help to refine and reduce the use of animals in experiments.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Center for Muscle Biology, University of Kentucky, Lexington, Kentucky charles.chung@uky.edu.

No MeSH data available.