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Effects of treatment with Astragalus Membranaceus on function of rat leydig cells.

Jiang X, Cao X, Huang Y, Chen J, Yao X, Zhao M, Liu Y, Meng J, Li P, Li Z, Yao J, Smith GW, Lv L - BMC Complement Altern Med (2015)

Bottom Line: In the present study, we examined the effects of AM on Leydig cell function in vitro.Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Shanxi Agricultural University, No.1 Mingxian Nan Road, Taigu, 030801, China. 1172754383@qq.com.

ABSTRACT

Background: Astragalus membranaceus (AM) is a Chinese traditional herb which has been reported to have broad positive effects on many diseases, including hepatitis, heart disease, diabetes and skin disease. AM can promote cell proliferation, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and inhibit apoptosis by regulating the transcription of proto-oncogenes controlling cell death. While AM is included in some commercially available "testosterone boosting supplements", studies directly testing ability of AM to modulate testosterone production are lacking. In the present study, we examined the effects of AM on Leydig cell function in vitro.

Methods: Rat Leydig cells were purified and treated with AM at different concentrations (0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL) and cell counting-8 (CCK-8) assay, Enzyme-linked immunosorbent assay, quantitative real time PCR and analysis of activities of SOD and GPx were done respectively.

Results: Treatment with 100 μg/mL (P<0.05) and 150 μg/mL AM (P<0.01) significantly increased Leydig cell numbers. Treatment with AM (20 μg/mL, 50 μg/mL and 100 μg/mL) significantly increased testosterone production (P<0.01). In addition, increased Leydig cell SOD and GPx activities were observed in response to 20 μg/mL and 50 μg/mL AM treatment (P<0.01). Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).

Conclusions: Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

No MeSH data available.


Related in: MedlinePlus

Effect of AM treatment on the expression of Bax and Bcl-2 in Leydig cells. qRT-PCR was performed to determine the abundances of Bax (a) and Bcl-2 (b) mRNA in Leydig cells treated with 20 μg/mL of AM, and to further determine the effect of AM treatment on expression level of Bax and Bcl-2, we calculated the ratio of Bcl-2 to Bax (c). Expression of Bax and Bcl-2 mRNA was normalized relative to the abundance of GAPDH mRNA. Results are depicted as mean +/- SE (n = 3). * P < 0.05, ** P < 0.01
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Fig5: Effect of AM treatment on the expression of Bax and Bcl-2 in Leydig cells. qRT-PCR was performed to determine the abundances of Bax (a) and Bcl-2 (b) mRNA in Leydig cells treated with 20 μg/mL of AM, and to further determine the effect of AM treatment on expression level of Bax and Bcl-2, we calculated the ratio of Bcl-2 to Bax (c). Expression of Bax and Bcl-2 mRNA was normalized relative to the abundance of GAPDH mRNA. Results are depicted as mean +/- SE (n = 3). * P < 0.05, ** P < 0.01

Mentions: Effect of AM on Bax and Bcl-2 mRNA expression in AM treated Leydig cells To further elucidate the potential effects of AM on Leydig cell numbers, the expression of apoptosis-related genes Bax and Bcl-2 in Leydig cells treated with 0 μg/mL or 20 μg/mL of AM injection for 48 h were analyzed using qRT-PCR. Results showed in Fig. 5 indicated the expression of Bax mRNA was significantly reduced in AM treated group (P < 0.01) compared with the untreated control, while the expression of Bcl-2 mRNA had no obvious change between AM treated group and the untreated control (P > 0.05). However, the ratio of Bcl-2/Bax mRNA was significantly higher in the AM treated group versus the untreated control (P < 0.05).Fig. 5


Effects of treatment with Astragalus Membranaceus on function of rat leydig cells.

Jiang X, Cao X, Huang Y, Chen J, Yao X, Zhao M, Liu Y, Meng J, Li P, Li Z, Yao J, Smith GW, Lv L - BMC Complement Altern Med (2015)

Effect of AM treatment on the expression of Bax and Bcl-2 in Leydig cells. qRT-PCR was performed to determine the abundances of Bax (a) and Bcl-2 (b) mRNA in Leydig cells treated with 20 μg/mL of AM, and to further determine the effect of AM treatment on expression level of Bax and Bcl-2, we calculated the ratio of Bcl-2 to Bax (c). Expression of Bax and Bcl-2 mRNA was normalized relative to the abundance of GAPDH mRNA. Results are depicted as mean +/- SE (n = 3). * P < 0.05, ** P < 0.01
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4522129&req=5

Fig5: Effect of AM treatment on the expression of Bax and Bcl-2 in Leydig cells. qRT-PCR was performed to determine the abundances of Bax (a) and Bcl-2 (b) mRNA in Leydig cells treated with 20 μg/mL of AM, and to further determine the effect of AM treatment on expression level of Bax and Bcl-2, we calculated the ratio of Bcl-2 to Bax (c). Expression of Bax and Bcl-2 mRNA was normalized relative to the abundance of GAPDH mRNA. Results are depicted as mean +/- SE (n = 3). * P < 0.05, ** P < 0.01
Mentions: Effect of AM on Bax and Bcl-2 mRNA expression in AM treated Leydig cells To further elucidate the potential effects of AM on Leydig cell numbers, the expression of apoptosis-related genes Bax and Bcl-2 in Leydig cells treated with 0 μg/mL or 20 μg/mL of AM injection for 48 h were analyzed using qRT-PCR. Results showed in Fig. 5 indicated the expression of Bax mRNA was significantly reduced in AM treated group (P < 0.01) compared with the untreated control, while the expression of Bcl-2 mRNA had no obvious change between AM treated group and the untreated control (P > 0.05). However, the ratio of Bcl-2/Bax mRNA was significantly higher in the AM treated group versus the untreated control (P < 0.05).Fig. 5

Bottom Line: In the present study, we examined the effects of AM on Leydig cell function in vitro.Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Shanxi Agricultural University, No.1 Mingxian Nan Road, Taigu, 030801, China. 1172754383@qq.com.

ABSTRACT

Background: Astragalus membranaceus (AM) is a Chinese traditional herb which has been reported to have broad positive effects on many diseases, including hepatitis, heart disease, diabetes and skin disease. AM can promote cell proliferation, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and inhibit apoptosis by regulating the transcription of proto-oncogenes controlling cell death. While AM is included in some commercially available "testosterone boosting supplements", studies directly testing ability of AM to modulate testosterone production are lacking. In the present study, we examined the effects of AM on Leydig cell function in vitro.

Methods: Rat Leydig cells were purified and treated with AM at different concentrations (0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL) and cell counting-8 (CCK-8) assay, Enzyme-linked immunosorbent assay, quantitative real time PCR and analysis of activities of SOD and GPx were done respectively.

Results: Treatment with 100 μg/mL (P<0.05) and 150 μg/mL AM (P<0.01) significantly increased Leydig cell numbers. Treatment with AM (20 μg/mL, 50 μg/mL and 100 μg/mL) significantly increased testosterone production (P<0.01). In addition, increased Leydig cell SOD and GPx activities were observed in response to 20 μg/mL and 50 μg/mL AM treatment (P<0.01). Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).

Conclusions: Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

No MeSH data available.


Related in: MedlinePlus