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Effects of treatment with Astragalus Membranaceus on function of rat leydig cells.

Jiang X, Cao X, Huang Y, Chen J, Yao X, Zhao M, Liu Y, Meng J, Li P, Li Z, Yao J, Smith GW, Lv L - BMC Complement Altern Med (2015)

Bottom Line: In the present study, we examined the effects of AM on Leydig cell function in vitro.Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Shanxi Agricultural University, No.1 Mingxian Nan Road, Taigu, 030801, China. 1172754383@qq.com.

ABSTRACT

Background: Astragalus membranaceus (AM) is a Chinese traditional herb which has been reported to have broad positive effects on many diseases, including hepatitis, heart disease, diabetes and skin disease. AM can promote cell proliferation, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and inhibit apoptosis by regulating the transcription of proto-oncogenes controlling cell death. While AM is included in some commercially available "testosterone boosting supplements", studies directly testing ability of AM to modulate testosterone production are lacking. In the present study, we examined the effects of AM on Leydig cell function in vitro.

Methods: Rat Leydig cells were purified and treated with AM at different concentrations (0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL) and cell counting-8 (CCK-8) assay, Enzyme-linked immunosorbent assay, quantitative real time PCR and analysis of activities of SOD and GPx were done respectively.

Results: Treatment with 100 μg/mL (P<0.05) and 150 μg/mL AM (P<0.01) significantly increased Leydig cell numbers. Treatment with AM (20 μg/mL, 50 μg/mL and 100 μg/mL) significantly increased testosterone production (P<0.01). In addition, increased Leydig cell SOD and GPx activities were observed in response to 20 μg/mL and 50 μg/mL AM treatment (P<0.01). Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).

Conclusions: Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

No MeSH data available.


Related in: MedlinePlus

Effect of AM treatment on viable Leydig cell numbers. Cells were treated with 0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL of AM. Cell viability was analyzed by CCK-8 assay. Results are depicted as mean +/- SE * P < 0.05, ** P < 0.01
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Fig2: Effect of AM treatment on viable Leydig cell numbers. Cells were treated with 0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL of AM. Cell viability was analyzed by CCK-8 assay. Results are depicted as mean +/- SE * P < 0.05, ** P < 0.01

Mentions: AM treatment increased Leydig cell numbers To investigate the effect of AM injection on Leydig cells, numbers of viable cells were determined by CCK-8 assay 48 h after AM treatment. Compared with the untreated control, numbers of viable cells increased within a certain range of concentration, particularly at the concentrations of 100 μg/mL (P < 0.05) and 150 μg/mL (P < 0.01) (Fig. 2). The result suggested that addition of AM increases numbers of viable Leydig cells 48 h after treatment.Fig. 2


Effects of treatment with Astragalus Membranaceus on function of rat leydig cells.

Jiang X, Cao X, Huang Y, Chen J, Yao X, Zhao M, Liu Y, Meng J, Li P, Li Z, Yao J, Smith GW, Lv L - BMC Complement Altern Med (2015)

Effect of AM treatment on viable Leydig cell numbers. Cells were treated with 0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL of AM. Cell viability was analyzed by CCK-8 assay. Results are depicted as mean +/- SE * P < 0.05, ** P < 0.01
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4522129&req=5

Fig2: Effect of AM treatment on viable Leydig cell numbers. Cells were treated with 0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL of AM. Cell viability was analyzed by CCK-8 assay. Results are depicted as mean +/- SE * P < 0.05, ** P < 0.01
Mentions: AM treatment increased Leydig cell numbers To investigate the effect of AM injection on Leydig cells, numbers of viable cells were determined by CCK-8 assay 48 h after AM treatment. Compared with the untreated control, numbers of viable cells increased within a certain range of concentration, particularly at the concentrations of 100 μg/mL (P < 0.05) and 150 μg/mL (P < 0.01) (Fig. 2). The result suggested that addition of AM increases numbers of viable Leydig cells 48 h after treatment.Fig. 2

Bottom Line: In the present study, we examined the effects of AM on Leydig cell function in vitro.Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Shanxi Agricultural University, No.1 Mingxian Nan Road, Taigu, 030801, China. 1172754383@qq.com.

ABSTRACT

Background: Astragalus membranaceus (AM) is a Chinese traditional herb which has been reported to have broad positive effects on many diseases, including hepatitis, heart disease, diabetes and skin disease. AM can promote cell proliferation, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and inhibit apoptosis by regulating the transcription of proto-oncogenes controlling cell death. While AM is included in some commercially available "testosterone boosting supplements", studies directly testing ability of AM to modulate testosterone production are lacking. In the present study, we examined the effects of AM on Leydig cell function in vitro.

Methods: Rat Leydig cells were purified and treated with AM at different concentrations (0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL) and cell counting-8 (CCK-8) assay, Enzyme-linked immunosorbent assay, quantitative real time PCR and analysis of activities of SOD and GPx were done respectively.

Results: Treatment with 100 μg/mL (P<0.05) and 150 μg/mL AM (P<0.01) significantly increased Leydig cell numbers. Treatment with AM (20 μg/mL, 50 μg/mL and 100 μg/mL) significantly increased testosterone production (P<0.01). In addition, increased Leydig cell SOD and GPx activities were observed in response to 20 μg/mL and 50 μg/mL AM treatment (P<0.01). Furthermore, expression of Bax mRNA was significantly decreased (P<0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P<0.05).

Conclusions: Results supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

No MeSH data available.


Related in: MedlinePlus