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Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan.

Khisroon M, Gul A, Khan A, Ali N, Zaidi F, Rasheed SB - J Occup Med Toxicol (2015)

Bottom Line: Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively).The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects.The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University of Peshawar, Peshawar, Pakistan.

ABSTRACT

Background: Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons.

Methods: Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.

Results: Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05).

Conclusions: The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.

No MeSH data available.


Related in: MedlinePlus

DNA damage assessed using the comet assay. Mean comet score and comet classes in control and exposed groups. The cells were assessed visually and received class 0 (undamaged) to 4 (maximally damaged), according to the size and shape of the tail. Score were obtained using the mean score of three independent, blind evaluators
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Fig1: DNA damage assessed using the comet assay. Mean comet score and comet classes in control and exposed groups. The cells were assessed visually and received class 0 (undamaged) to 4 (maximally damaged), according to the size and shape of the tail. Score were obtained using the mean score of three independent, blind evaluators

Mentions: The slides were stained with 70 μl Acridine orange dye (20 μg/ml) and kept for 5 min. Cover slips were placed on it, and viewed at 200x of fluorescent microscope (Nikon Eclipse 80 i) equipped with 450-490 nm excitation filter. In order to calculate DNA damage, 100 cells per sample were chosen randomly and analyzed visually according to comet appearance. 5 classes, i.e. from class 0 (no DNA damage) to class 4 (maximum DNA damage) give sufficient declaration (Fig. 1). Visual scoring is a reliable, simple and rapid method for scoring the comets [18]. Total comet score (TCS) was then calculated according to the formula, TCS = 0(n) +1(n) +2(n) +3(n) +4(n), where “n” indicates number of cells in each class [18].Fig. 1


Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan.

Khisroon M, Gul A, Khan A, Ali N, Zaidi F, Rasheed SB - J Occup Med Toxicol (2015)

DNA damage assessed using the comet assay. Mean comet score and comet classes in control and exposed groups. The cells were assessed visually and received class 0 (undamaged) to 4 (maximally damaged), according to the size and shape of the tail. Score were obtained using the mean score of three independent, blind evaluators
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4522117&req=5

Fig1: DNA damage assessed using the comet assay. Mean comet score and comet classes in control and exposed groups. The cells were assessed visually and received class 0 (undamaged) to 4 (maximally damaged), according to the size and shape of the tail. Score were obtained using the mean score of three independent, blind evaluators
Mentions: The slides were stained with 70 μl Acridine orange dye (20 μg/ml) and kept for 5 min. Cover slips were placed on it, and viewed at 200x of fluorescent microscope (Nikon Eclipse 80 i) equipped with 450-490 nm excitation filter. In order to calculate DNA damage, 100 cells per sample were chosen randomly and analyzed visually according to comet appearance. 5 classes, i.e. from class 0 (no DNA damage) to class 4 (maximum DNA damage) give sufficient declaration (Fig. 1). Visual scoring is a reliable, simple and rapid method for scoring the comets [18]. Total comet score (TCS) was then calculated according to the formula, TCS = 0(n) +1(n) +2(n) +3(n) +4(n), where “n” indicates number of cells in each class [18].Fig. 1

Bottom Line: Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively).The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects.The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University of Peshawar, Peshawar, Pakistan.

ABSTRACT

Background: Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons.

Methods: Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.

Results: Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05).

Conclusions: The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.

No MeSH data available.


Related in: MedlinePlus