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Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.

Jumaah F, Sandahl M, Turner C - J Am Oil Chem Soc (2015)

Bottom Line: Best SFE condition for total lipids was 450 bar, 60 °C and 45 min.The SFE method was compared to conventional Bligh & Dyer (B&D) extraction.The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Centre for Analysis and Synthesis, Lund University, P.O. Box 124, 22100 Lund, Sweden.

ABSTRACT

A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.

No MeSH data available.


Related in: MedlinePlus

Scheme of the SFE equipment: (1) Liquid CO2 cylinder with a dip tube; (2) valve; (3) CO2 syringe pump (model ISCO 260 D, Teledyne ISCO, Thousand Oaks, CA) with a cooling jacket; (4) check valve; (5) extraction vessel (10 ml); (6) extraction oven (SFX 2-10); (7) restrictor; (8) heater; and (9) collection vial with solvent
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Fig1: Scheme of the SFE equipment: (1) Liquid CO2 cylinder with a dip tube; (2) valve; (3) CO2 syringe pump (model ISCO 260 D, Teledyne ISCO, Thousand Oaks, CA) with a cooling jacket; (4) check valve; (5) extraction vessel (10 ml); (6) extraction oven (SFX 2-10); (7) restrictor; (8) heater; and (9) collection vial with solvent

Mentions: SFE extraction experiments were carried out using SC-CO2 as a solvent. A scheme of the ISCO SFE apparatus is presented in Fig. 1.Fig. 1


Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.

Jumaah F, Sandahl M, Turner C - J Am Oil Chem Soc (2015)

Scheme of the SFE equipment: (1) Liquid CO2 cylinder with a dip tube; (2) valve; (3) CO2 syringe pump (model ISCO 260 D, Teledyne ISCO, Thousand Oaks, CA) with a cooling jacket; (4) check valve; (5) extraction vessel (10 ml); (6) extraction oven (SFX 2-10); (7) restrictor; (8) heater; and (9) collection vial with solvent
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4522036&req=5

Fig1: Scheme of the SFE equipment: (1) Liquid CO2 cylinder with a dip tube; (2) valve; (3) CO2 syringe pump (model ISCO 260 D, Teledyne ISCO, Thousand Oaks, CA) with a cooling jacket; (4) check valve; (5) extraction vessel (10 ml); (6) extraction oven (SFX 2-10); (7) restrictor; (8) heater; and (9) collection vial with solvent
Mentions: SFE extraction experiments were carried out using SC-CO2 as a solvent. A scheme of the ISCO SFE apparatus is presented in Fig. 1.Fig. 1

Bottom Line: Best SFE condition for total lipids was 450 bar, 60 °C and 45 min.The SFE method was compared to conventional Bligh & Dyer (B&D) extraction.The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Centre for Analysis and Synthesis, Lund University, P.O. Box 124, 22100 Lund, Sweden.

ABSTRACT

A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.

No MeSH data available.


Related in: MedlinePlus