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Circulating CCR7+ICOS+ Memory T Follicular Helper Cells in Patients with Multiple Sclerosis.

Fan X, Jin T, Zhao S, Liu C, Han J, Jiang X, Jiang Y - PLoS ONE (2015)

Bottom Line: In comparison with that in the HC, the numbers of circulating CD3+CD4+CXCR5+CD45RA-, ICOS+, CCR7+ and CCR7+ICOS+ memory Tfh cells and the levels of plasma IL-21 significantly increased in MS patients, but significantly decreased in the patients with complete remission (CR).The levels of CSF IL-21 were significantly higher in the MS patients than that in the NND patients.Our findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Jilin University, Changchun, China.

ABSTRACT

Objective: This study is aimed at examining the potential roles of circulating memory T follicular helper (Tfh) cells in patients with multiple sclerosis (MS).

Methods: The numbers of different subsets of circulating memory Tfh cells in 25 patients with relapsed MS before and after treatment as well as 14 healthy controls (HC) were examined by flow cytometry. The levels of plasma IL-21 in all patients and cerebrospinal fluid (CSF) IL-21 in some MS patients and controls with non-inflammatory neuronal diseases (NND) were measured by ELISA.

Results: In comparison with that in the HC, the numbers of circulating CD3+CD4+CXCR5+CD45RA-, ICOS+, CCR7+ and CCR7+ICOS+ memory Tfh cells and the levels of plasma IL-21 significantly increased in MS patients, but significantly decreased in the patients with complete remission (CR). The levels of CSF IL-21 were significantly higher in the MS patients than that in the NND patients. The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the EDSS scores, the levels of plasma and CSF IL-21, IgG, MBP-Ab or MOG-Ab.

Conclusions: Our findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target.

No MeSH data available.


Related in: MedlinePlus

Correlation analysis of the numbers of different subsets of circulating memory Tfh cells and the values of clinical measures in MS patients.(A-B) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the EDSS scores in MS patients. (C-D) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the levels of plasma IL-21. (E-F) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF IL-21 and CSF IgG. (G-H) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab.
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pone.0134523.g004: Correlation analysis of the numbers of different subsets of circulating memory Tfh cells and the values of clinical measures in MS patients.(A-B) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the EDSS scores in MS patients. (C-D) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the levels of plasma IL-21. (E-F) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF IL-21 and CSF IgG. (G-H) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab.

Mentions: To understand the potential importance of memory Tfh cells, we analyzed the relationship between the numbers of memory Tfh cells and the values of clinical measures tested in the MS patients. We found that the numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively correlated with the EDSS scores and the levels of plasma IL-21 in the MS patients (Fig 4A–4D). Furthermore, there was a significant correlation between the number of CCR7+ICOS+ memory Tfh cells and the levels of CSF IL-21 or IgG in the MS patients (Fig 4E and 4F). In addition, the numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab (Fig 4G and 4H). However, there was no correlation among the values of other measures tested.


Circulating CCR7+ICOS+ Memory T Follicular Helper Cells in Patients with Multiple Sclerosis.

Fan X, Jin T, Zhao S, Liu C, Han J, Jiang X, Jiang Y - PLoS ONE (2015)

Correlation analysis of the numbers of different subsets of circulating memory Tfh cells and the values of clinical measures in MS patients.(A-B) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the EDSS scores in MS patients. (C-D) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the levels of plasma IL-21. (E-F) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF IL-21 and CSF IgG. (G-H) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4521720&req=5

pone.0134523.g004: Correlation analysis of the numbers of different subsets of circulating memory Tfh cells and the values of clinical measures in MS patients.(A-B) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the EDSS scores in MS patients. (C-D) The numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively associated with the levels of plasma IL-21. (E-F) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF IL-21 and CSF IgG. (G-H) The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab.
Mentions: To understand the potential importance of memory Tfh cells, we analyzed the relationship between the numbers of memory Tfh cells and the values of clinical measures tested in the MS patients. We found that the numbers of ICOS+ and CCR7+ICOS+ memory Tfh cells were positively correlated with the EDSS scores and the levels of plasma IL-21 in the MS patients (Fig 4A–4D). Furthermore, there was a significant correlation between the number of CCR7+ICOS+ memory Tfh cells and the levels of CSF IL-21 or IgG in the MS patients (Fig 4E and 4F). In addition, the numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the levels of CSF MBP-Ab and CSF MOG-Ab (Fig 4G and 4H). However, there was no correlation among the values of other measures tested.

Bottom Line: In comparison with that in the HC, the numbers of circulating CD3+CD4+CXCR5+CD45RA-, ICOS+, CCR7+ and CCR7+ICOS+ memory Tfh cells and the levels of plasma IL-21 significantly increased in MS patients, but significantly decreased in the patients with complete remission (CR).The levels of CSF IL-21 were significantly higher in the MS patients than that in the NND patients.Our findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target.

View Article: PubMed Central - PubMed

Affiliation: Neuroscience Center, Department of Neurology, the First Hospital of Jilin University, Jilin University, Changchun, China.

ABSTRACT

Objective: This study is aimed at examining the potential roles of circulating memory T follicular helper (Tfh) cells in patients with multiple sclerosis (MS).

Methods: The numbers of different subsets of circulating memory Tfh cells in 25 patients with relapsed MS before and after treatment as well as 14 healthy controls (HC) were examined by flow cytometry. The levels of plasma IL-21 in all patients and cerebrospinal fluid (CSF) IL-21 in some MS patients and controls with non-inflammatory neuronal diseases (NND) were measured by ELISA.

Results: In comparison with that in the HC, the numbers of circulating CD3+CD4+CXCR5+CD45RA-, ICOS+, CCR7+ and CCR7+ICOS+ memory Tfh cells and the levels of plasma IL-21 significantly increased in MS patients, but significantly decreased in the patients with complete remission (CR). The levels of CSF IL-21 were significantly higher in the MS patients than that in the NND patients. The numbers of CCR7+ICOS+ memory Tfh cells were positively correlated with the EDSS scores, the levels of plasma and CSF IL-21, IgG, MBP-Ab or MOG-Ab.

Conclusions: Our findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target.

No MeSH data available.


Related in: MedlinePlus