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Protection of Gastrointestinal Mucosa from Acute Heavy Alcohol Consumption: The Effect of Berberine and Its Correlation with TLR2, 4/IL1β-TNFα Signaling.

Wang XP, Lei F, Du F, Chai YS, Jiang JF, Wang YG, Yu X, Yan XJ, Xing DM, Du LJ - PLoS ONE (2015)

Bottom Line: The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously.Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa.Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

ABSTRACT
The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously. Our research details how BBR protects against gastrointestinal injuries from acute alcohol exposure using both in vivo and in vitro experiments. Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa. Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions. Alcohol consumption is a popular human social behavior worldwide, and the present study reports a comprehensive mechanism by which BBR protects against gastrointestinal injuries from alcohol stress, providing people with a novel application of BBR.

No MeSH data available.


Related in: MedlinePlus

The expressions of inflammatory cytokines of mouse small intestines after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
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pone.0134044.g004: The expressions of inflammatory cytokines of mouse small intestines after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.

Mentions: Consistent with the studies in stomach, acute alcohol administration significantly increased the expressions of TNFα, IL1-β, TLR2, and TLR4 at the mRNA and protein level (Fig 4), and BBR dose-dependently inhibited alcohol-induced changes. However, inconsistent with NOD2 expression in the stomach, BBR inhibited the mRNA and protein upregulation of NOD2 caused by alcohol (Fig 4C& 4J), suggesting the greater sensitivity of the NOD2 response of the small intestinal mucosa to alcohol stress. The expression of other proteins was consistent with the mRNA results. In the small intestine, alcohol administration suppressed the expression of occludin but increased the expression of claudin4. BBR enhanced occludin expression and attenuated claudin4 expression (Fig 4F and 4G & 4M and 4N).


Protection of Gastrointestinal Mucosa from Acute Heavy Alcohol Consumption: The Effect of Berberine and Its Correlation with TLR2, 4/IL1β-TNFα Signaling.

Wang XP, Lei F, Du F, Chai YS, Jiang JF, Wang YG, Yu X, Yan XJ, Xing DM, Du LJ - PLoS ONE (2015)

The expressions of inflammatory cytokines of mouse small intestines after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4520689&req=5

pone.0134044.g004: The expressions of inflammatory cytokines of mouse small intestines after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
Mentions: Consistent with the studies in stomach, acute alcohol administration significantly increased the expressions of TNFα, IL1-β, TLR2, and TLR4 at the mRNA and protein level (Fig 4), and BBR dose-dependently inhibited alcohol-induced changes. However, inconsistent with NOD2 expression in the stomach, BBR inhibited the mRNA and protein upregulation of NOD2 caused by alcohol (Fig 4C& 4J), suggesting the greater sensitivity of the NOD2 response of the small intestinal mucosa to alcohol stress. The expression of other proteins was consistent with the mRNA results. In the small intestine, alcohol administration suppressed the expression of occludin but increased the expression of claudin4. BBR enhanced occludin expression and attenuated claudin4 expression (Fig 4F and 4G & 4M and 4N).

Bottom Line: The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously.Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa.Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

ABSTRACT
The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously. Our research details how BBR protects against gastrointestinal injuries from acute alcohol exposure using both in vivo and in vitro experiments. Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa. Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions. Alcohol consumption is a popular human social behavior worldwide, and the present study reports a comprehensive mechanism by which BBR protects against gastrointestinal injuries from alcohol stress, providing people with a novel application of BBR.

No MeSH data available.


Related in: MedlinePlus