Limits...
Protection of Gastrointestinal Mucosa from Acute Heavy Alcohol Consumption: The Effect of Berberine and Its Correlation with TLR2, 4/IL1β-TNFα Signaling.

Wang XP, Lei F, Du F, Chai YS, Jiang JF, Wang YG, Yu X, Yan XJ, Xing DM, Du LJ - PLoS ONE (2015)

Bottom Line: The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously.Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa.Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

ABSTRACT
The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously. Our research details how BBR protects against gastrointestinal injuries from acute alcohol exposure using both in vivo and in vitro experiments. Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa. Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions. Alcohol consumption is a popular human social behavior worldwide, and the present study reports a comprehensive mechanism by which BBR protects against gastrointestinal injuries from alcohol stress, providing people with a novel application of BBR.

No MeSH data available.


Related in: MedlinePlus

The expressions of inflammatory cytokines of mouse stomach after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4520689&req=5

pone.0134044.g003: The expressions of inflammatory cytokines of mouse stomach after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.

Mentions: To understand the inflammatory response and abnormal expression of pattern recognition receptors (e.g., Toll-like receptors (TLRs) and NOD2) accompanying the ethanol-induced gastrointestinal mucosal injury, we studied the expression of TLR2, TLR4 and NOD2 and their down-stream effectors TNFα and IL1-β in mouse stomachs after acute alcohol exposure by q-PCR and western blot. The results revealed that acute alcohol exposure could significantly up-regulate the transcription and protein level of TNFα, IL1-β, TLR2, and TLR4, and these alcohol-dependent enhancements were antagonized by BBR administration (Fig 3). Only one BBR dose group exhibited decreased expression of NOD2 protein as well as consistent q-PCR results, suggesting that BBR exhibits a greater effect on TLR2 and TLR4 compared with NOD2 in the stomach mucosa. Occludin and claudin4 are major components of tight conjunctions in the gastrointestinal epithelium and act to regulate intestinal epithelial permeability. Acute high alcohol exposure can up-regulate occludin and claudin4 expression. However, alcohol-induced expression profile changes could be antagonized by BBR, which attenuated occludin and claudin4 expression (Fig 3F and 3G & 3M and 3N).


Protection of Gastrointestinal Mucosa from Acute Heavy Alcohol Consumption: The Effect of Berberine and Its Correlation with TLR2, 4/IL1β-TNFα Signaling.

Wang XP, Lei F, Du F, Chai YS, Jiang JF, Wang YG, Yu X, Yan XJ, Xing DM, Du LJ - PLoS ONE (2015)

The expressions of inflammatory cytokines of mouse stomach after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520689&req=5

pone.0134044.g003: The expressions of inflammatory cytokines of mouse stomach after acute alcohol exposure.The concentration of alcohol was 60%. (A–G): mRNA expressions using real time PCR assay. (H–N): The expressions of protein using western blot assay. Berberine (BBR) was administered at three different doses of 75,150 and 300 mg/kg. Data are expressed as the mean ± S.D. from six different mice. ## vs. normal mice, P < 0.01. *, ** vs. model mice, P < 0.05, P < 0.01.
Mentions: To understand the inflammatory response and abnormal expression of pattern recognition receptors (e.g., Toll-like receptors (TLRs) and NOD2) accompanying the ethanol-induced gastrointestinal mucosal injury, we studied the expression of TLR2, TLR4 and NOD2 and their down-stream effectors TNFα and IL1-β in mouse stomachs after acute alcohol exposure by q-PCR and western blot. The results revealed that acute alcohol exposure could significantly up-regulate the transcription and protein level of TNFα, IL1-β, TLR2, and TLR4, and these alcohol-dependent enhancements were antagonized by BBR administration (Fig 3). Only one BBR dose group exhibited decreased expression of NOD2 protein as well as consistent q-PCR results, suggesting that BBR exhibits a greater effect on TLR2 and TLR4 compared with NOD2 in the stomach mucosa. Occludin and claudin4 are major components of tight conjunctions in the gastrointestinal epithelium and act to regulate intestinal epithelial permeability. Acute high alcohol exposure can up-regulate occludin and claudin4 expression. However, alcohol-induced expression profile changes could be antagonized by BBR, which attenuated occludin and claudin4 expression (Fig 3F and 3G & 3M and 3N).

Bottom Line: The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously.Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa.Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Laboratory of Protein Sciences, Laboratory of Molecular Pharmacology and Pharmaceutical Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

ABSTRACT
The purpose of the present study is to confirm the protective effect of berberine (BBR) on gastrointestinal injury caused by acute heavy alcohol exposure, an effect that has not been reported previously. Our research details how BBR protects against gastrointestinal injuries from acute alcohol exposure using both in vivo and in vitro experiments. Acute high alcohol concentrations lead to obvious damage to the gastrointestinal mucosa, resulting in necrosis of the intestinal mucosa. Oral administration of BBR was able to significantly reduce this alcohol-induced damage, inhibit increases of alcohol-induced TNFα and IL-1β expression in gastrointestinal mucosa as well as their upstream signals TLR2 and TLR4, and regulate cytokines that modulate tight junctions. Alcohol consumption is a popular human social behavior worldwide, and the present study reports a comprehensive mechanism by which BBR protects against gastrointestinal injuries from alcohol stress, providing people with a novel application of BBR.

No MeSH data available.


Related in: MedlinePlus