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High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients.

Kawate T, Iwaya K, Koshikawa K, Moriya T, Yamasaki T, Hasegawa S, Kaise H, Fujita T, Matsuo H, Nakamura T, Ishikawa T, Hiroi S, Iguchi-Ariga SM, Ariga H, Murota K, Fujimori M, Yamamoto J, Matsubara O, Kohno N - Cancer Sci. (2015)

Bottom Line: Higher DJ-1 levels were significantly associated with advanced clinical grade, according to the TNM classification, negative hormone receptor status, and high Ki-67 labeling index, of biopsied materials; samples showed low DJ-1 protein expression despite upregulated DJ-1 mRNA.DJ-1 isoforms could be detected clearly in 17 blood samples (from 11 breast cancer patients, and 6 non-cancerous controls) by 2-D gel electrophoresis and immunoblot analysis.Conversely, in the 6 non-cancerous cases, isoforms other than the pI 6.3 isoform were highly expressed, and there was a significant difference in the isoform pattern between breast cancer cases and controls (P = 0.00025).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, National Defense Medical College, Saitama, Japan.

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Related in: MedlinePlus

Expression of DJ-1 protein and mRNA in a breast cancer biopsy specimen. (a) The breast cancer nest is located in the center of the H&E stained specimen. Non-cancerous ducts are seen in the right lower corner. Bar = 50 μm (originally 400 high power fields). (b) Immunohistochemistry of DJ-1, in the same area as in (a). The immunoreactivity in the cancerous area is marked by arrowheads, and was analyzed by the image analyzer WinROOF. The average intensity of DJ-1 staining in the non-cancerous ducts located in the right corner was determined as the threshold value by applying the hue–lightness–saturation color space. The expression of DJ-1 in the cancerous area was judged as low because the intensity of DJ-1 staining was lower than the threshold value in the cancerous area. (c) In situ hybridization of DJ-1, in the same area as in (a, b). Upregulation of DJ-1 mRNA was detected in the cancerous lesion (arrowheads).
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fig02: Expression of DJ-1 protein and mRNA in a breast cancer biopsy specimen. (a) The breast cancer nest is located in the center of the H&E stained specimen. Non-cancerous ducts are seen in the right lower corner. Bar = 50 μm (originally 400 high power fields). (b) Immunohistochemistry of DJ-1, in the same area as in (a). The immunoreactivity in the cancerous area is marked by arrowheads, and was analyzed by the image analyzer WinROOF. The average intensity of DJ-1 staining in the non-cancerous ducts located in the right corner was determined as the threshold value by applying the hue–lightness–saturation color space. The expression of DJ-1 in the cancerous area was judged as low because the intensity of DJ-1 staining was lower than the threshold value in the cancerous area. (c) In situ hybridization of DJ-1, in the same area as in (a, b). Upregulation of DJ-1 mRNA was detected in the cancerous lesion (arrowheads).

Mentions: In situ hybridization of the needle biopsies revealed upregulated levels of DJ-1 mRNA in breast cancer tissue in 171 (98.8%) of 173 pre-therapeutic samples. At the protein level, low expression of DJ-1 was detected in 85 of 173 (49.1%) breast cancer cases by semiquantitative immunohistochemical analysis. Figure2 shows a breast cancer case with high DJ-1 mRNA levels and low expression of DJ-1 protein, whose expression pattern has been observed with secretion and with high DJ-1 levels in the nipple discharge, reported previously.9,10 In cases with low expression of DJ-1 protein, the mean level of DJ-1 protein in the sera was 67.0 ng/mL, significantly higher than that in cases with high expression of DJ-1 protein (23.5 ng/mL).


High levels of DJ-1 protein and isoelectric point 6.3 isoform in sera of breast cancer patients.

Kawate T, Iwaya K, Koshikawa K, Moriya T, Yamasaki T, Hasegawa S, Kaise H, Fujita T, Matsuo H, Nakamura T, Ishikawa T, Hiroi S, Iguchi-Ariga SM, Ariga H, Murota K, Fujimori M, Yamamoto J, Matsubara O, Kohno N - Cancer Sci. (2015)

Expression of DJ-1 protein and mRNA in a breast cancer biopsy specimen. (a) The breast cancer nest is located in the center of the H&E stained specimen. Non-cancerous ducts are seen in the right lower corner. Bar = 50 μm (originally 400 high power fields). (b) Immunohistochemistry of DJ-1, in the same area as in (a). The immunoreactivity in the cancerous area is marked by arrowheads, and was analyzed by the image analyzer WinROOF. The average intensity of DJ-1 staining in the non-cancerous ducts located in the right corner was determined as the threshold value by applying the hue–lightness–saturation color space. The expression of DJ-1 in the cancerous area was judged as low because the intensity of DJ-1 staining was lower than the threshold value in the cancerous area. (c) In situ hybridization of DJ-1, in the same area as in (a, b). Upregulation of DJ-1 mRNA was detected in the cancerous lesion (arrowheads).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520647&req=5

fig02: Expression of DJ-1 protein and mRNA in a breast cancer biopsy specimen. (a) The breast cancer nest is located in the center of the H&E stained specimen. Non-cancerous ducts are seen in the right lower corner. Bar = 50 μm (originally 400 high power fields). (b) Immunohistochemistry of DJ-1, in the same area as in (a). The immunoreactivity in the cancerous area is marked by arrowheads, and was analyzed by the image analyzer WinROOF. The average intensity of DJ-1 staining in the non-cancerous ducts located in the right corner was determined as the threshold value by applying the hue–lightness–saturation color space. The expression of DJ-1 in the cancerous area was judged as low because the intensity of DJ-1 staining was lower than the threshold value in the cancerous area. (c) In situ hybridization of DJ-1, in the same area as in (a, b). Upregulation of DJ-1 mRNA was detected in the cancerous lesion (arrowheads).
Mentions: In situ hybridization of the needle biopsies revealed upregulated levels of DJ-1 mRNA in breast cancer tissue in 171 (98.8%) of 173 pre-therapeutic samples. At the protein level, low expression of DJ-1 was detected in 85 of 173 (49.1%) breast cancer cases by semiquantitative immunohistochemical analysis. Figure2 shows a breast cancer case with high DJ-1 mRNA levels and low expression of DJ-1 protein, whose expression pattern has been observed with secretion and with high DJ-1 levels in the nipple discharge, reported previously.9,10 In cases with low expression of DJ-1 protein, the mean level of DJ-1 protein in the sera was 67.0 ng/mL, significantly higher than that in cases with high expression of DJ-1 protein (23.5 ng/mL).

Bottom Line: Higher DJ-1 levels were significantly associated with advanced clinical grade, according to the TNM classification, negative hormone receptor status, and high Ki-67 labeling index, of biopsied materials; samples showed low DJ-1 protein expression despite upregulated DJ-1 mRNA.DJ-1 isoforms could be detected clearly in 17 blood samples (from 11 breast cancer patients, and 6 non-cancerous controls) by 2-D gel electrophoresis and immunoblot analysis.Conversely, in the 6 non-cancerous cases, isoforms other than the pI 6.3 isoform were highly expressed, and there was a significant difference in the isoform pattern between breast cancer cases and controls (P = 0.00025).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, National Defense Medical College, Saitama, Japan.

Show MeSH
Related in: MedlinePlus