MT95-4, a fully humanized antibody raised against aminopeptidase N, reduces tumor progression in a mouse model.
Bottom Line: B16 mouse melanoma cells stably expressing human APN/CD13 were also established and were inoculated s.c. or injected i.v. into nude mice.We found that expression of human APN/CD13 in murine melanoma cells increased the size of subcutaneous tumors, extent of lung metastasis and degree of angiogenesis in the subcutaneous tumors; these tumor-promoting and angiogenesis-promoting characteristics were reduced by the i.p. administration of MT95-4.MT95-4 reduced tumor growth and angiogenesis in mice bearing H1299-derived and PC14-derived tumors, but not in mice bearing A549-derived tumors.
Affiliation: Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.Show MeSH
Related in: MedlinePlus
Mentions: To confirm the neutralizing effect of MT95-4 against APN/CD13 in vivo, we established a tail vein metastasis model and a subcutaneous xenograft model using APN-B16 cells and administered MT95-4 (1 mg/kg) i.p. twice per week in tumor-bearing mice. As shown in Figure3a and b, administration of MT95-4 reduced the number of lung surface nodules and the size of subcutaneous tumors in mice bearing APN-B16 cells. In addition, the sections of subcutaneous tumors consisting of APN-B16 cells were immunohistochemically stained with anti-CD31 antibodies, and the areas of CD31-positive vessels were significantly smaller in the MT95-4-treated group than in the control group (Fig.3c,d).
Affiliation: Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.