MT95-4, a fully humanized antibody raised against aminopeptidase N, reduces tumor progression in a mouse model.
Bottom Line: B16 mouse melanoma cells stably expressing human APN/CD13 were also established and were inoculated s.c. or injected i.v. into nude mice.We found that expression of human APN/CD13 in murine melanoma cells increased the size of subcutaneous tumors, extent of lung metastasis and degree of angiogenesis in the subcutaneous tumors; these tumor-promoting and angiogenesis-promoting characteristics were reduced by the i.p. administration of MT95-4.MT95-4 reduced tumor growth and angiogenesis in mice bearing H1299-derived and PC14-derived tumors, but not in mice bearing A549-derived tumors.
Affiliation: Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.Show MeSH
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Mentions: We established two B16-F1-derived cell lines that differed in the expression levels of human APN/CD13 (control-B16 and APN-B16 cells). Quantitative real-time PCR and flow cytometric analysis revealed that APN-B16 cells expressed high levels of human APN/CD13, while control-B16 cells did not (Fig.2a,b). When the in vitro cell proliferation rates of the two cell lines were compared, no differences were observed (data not shown).
Affiliation: Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.