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Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus

Networks of cytokines associated with Infective endocarditis.The groups evaluated were: health controls (HC), infections endocarditis (IE) and etiological subgroups: non-staphylococcal IE (non-S IE) and staphylococcal IE (S IE). Biomarkers networks were assembled to assess the association between human cytokines for each clinical group. The biomarkers networks were constructed using circle layouts with each cytokines represented by specific gray-scale globular nodes.
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pone.0133631.g005: Networks of cytokines associated with Infective endocarditis.The groups evaluated were: health controls (HC), infections endocarditis (IE) and etiological subgroups: non-staphylococcal IE (non-S IE) and staphylococcal IE (S IE). Biomarkers networks were assembled to assess the association between human cytokines for each clinical group. The biomarkers networks were constructed using circle layouts with each cytokines represented by specific gray-scale globular nodes.

Mentions: Biomarkers networks graphs were constructed to demonstrate the correlation between the pro-inflammatory, regulatory, and balanced cytokines with each other. The network data highlight the loss of interactions between the inflammatory cytokines and IL10 in staphylococcal IE. As a consequence, stronger correlations among the inflammatory cytokines with each other were developed in this subgroup of IE (Fig 5).


Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Networks of cytokines associated with Infective endocarditis.The groups evaluated were: health controls (HC), infections endocarditis (IE) and etiological subgroups: non-staphylococcal IE (non-S IE) and staphylococcal IE (S IE). Biomarkers networks were assembled to assess the association between human cytokines for each clinical group. The biomarkers networks were constructed using circle layouts with each cytokines represented by specific gray-scale globular nodes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520569&req=5

pone.0133631.g005: Networks of cytokines associated with Infective endocarditis.The groups evaluated were: health controls (HC), infections endocarditis (IE) and etiological subgroups: non-staphylococcal IE (non-S IE) and staphylococcal IE (S IE). Biomarkers networks were assembled to assess the association between human cytokines for each clinical group. The biomarkers networks were constructed using circle layouts with each cytokines represented by specific gray-scale globular nodes.
Mentions: Biomarkers networks graphs were constructed to demonstrate the correlation between the pro-inflammatory, regulatory, and balanced cytokines with each other. The network data highlight the loss of interactions between the inflammatory cytokines and IL10 in staphylococcal IE. As a consequence, stronger correlations among the inflammatory cytokines with each other were developed in this subgroup of IE (Fig 5).

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus