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Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus

Radar graphics showing the proportion of subjects and the immunological biomarkers production in infective endocarditis (IE) etiological subgroups.The subgroups evaluated were: culture-negative IE (CN-IE), non-staphylococcal IE (non-S IE), and staphylococcal IE (S IE). Elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the S IE subgroup. The prevalence of high producers of IL-10 was lower in the S IE subgroup as compared to the others subgroups.
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pone.0133631.g004: Radar graphics showing the proportion of subjects and the immunological biomarkers production in infective endocarditis (IE) etiological subgroups.The subgroups evaluated were: culture-negative IE (CN-IE), non-staphylococcal IE (non-S IE), and staphylococcal IE (S IE). Elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the S IE subgroup. The prevalence of high producers of IL-10 was lower in the S IE subgroup as compared to the others subgroups.

Mentions: In order to assess the levels of the cytokines in the patients of the IE group according to the infecting microorganism, the patients were stratified into three subgroups: staphylococcal IE, non-staphylococcal IE and culture-negative IE. The results are presented in Fig 3. Serum concentrations of TNF-α and IL-12 were significantly higher in the staphylococcal IE subgroup than in the non-staphylococcal IE group. When the prevalence of high producers of the immunological biomarkers among the IE subgroups was investigated (Fig 4), elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the staphylococcal IE subgroup. In addition, according to Fig 4, the prevalence of high producers of IL-10, an immunoregulatory cytokine, was lower in the staphylococcal IE subgroup. Finally, data from Figs 1 and 3 suggest that there are three distinct clustering of patients: those who present low, medium or high cytokine levels.


Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Radar graphics showing the proportion of subjects and the immunological biomarkers production in infective endocarditis (IE) etiological subgroups.The subgroups evaluated were: culture-negative IE (CN-IE), non-staphylococcal IE (non-S IE), and staphylococcal IE (S IE). Elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the S IE subgroup. The prevalence of high producers of IL-10 was lower in the S IE subgroup as compared to the others subgroups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520569&req=5

pone.0133631.g004: Radar graphics showing the proportion of subjects and the immunological biomarkers production in infective endocarditis (IE) etiological subgroups.The subgroups evaluated were: culture-negative IE (CN-IE), non-staphylococcal IE (non-S IE), and staphylococcal IE (S IE). Elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the S IE subgroup. The prevalence of high producers of IL-10 was lower in the S IE subgroup as compared to the others subgroups.
Mentions: In order to assess the levels of the cytokines in the patients of the IE group according to the infecting microorganism, the patients were stratified into three subgroups: staphylococcal IE, non-staphylococcal IE and culture-negative IE. The results are presented in Fig 3. Serum concentrations of TNF-α and IL-12 were significantly higher in the staphylococcal IE subgroup than in the non-staphylococcal IE group. When the prevalence of high producers of the immunological biomarkers among the IE subgroups was investigated (Fig 4), elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed only in the staphylococcal IE subgroup. In addition, according to Fig 4, the prevalence of high producers of IL-10, an immunoregulatory cytokine, was lower in the staphylococcal IE subgroup. Finally, data from Figs 1 and 3 suggest that there are three distinct clustering of patients: those who present low, medium or high cytokine levels.

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus