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Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus

Radar graphics showing the proportion of subjects and the immunological biomarkers production in different contexts.None of the individuals could be identified as high producers of cytokines in the health controls (HC) group. The infective endocarditis (IE) group presented high producers of all cytokines. The non-IE infections group presented high producers of IL-6 and IL-8.
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pone.0133631.g002: Radar graphics showing the proportion of subjects and the immunological biomarkers production in different contexts.None of the individuals could be identified as high producers of cytokines in the health controls (HC) group. The infective endocarditis (IE) group presented high producers of all cytokines. The non-IE infections group presented high producers of IL-6 and IL-8.

Mentions: Another way of presenting the results of the serum concentrations of the cytokines is to point out the high producers of these immunological biomarkers in the three study groups. These results are shown in Fig 2. These data complete and illustrate the information given in Fig 1. The IE group included high producers of all cytokines analyzed. The control group of patients with infections other than IE showed an elevated proportion of high producers of IL-6 and IL-8 and a moderate prevalence of high producers of IL-10. When the IE group and the group of other infections were compared, a more elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed in the IE group. There were high producers only of IL-10 in the group of healthy controls.


Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Radar graphics showing the proportion of subjects and the immunological biomarkers production in different contexts.None of the individuals could be identified as high producers of cytokines in the health controls (HC) group. The infective endocarditis (IE) group presented high producers of all cytokines. The non-IE infections group presented high producers of IL-6 and IL-8.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520569&req=5

pone.0133631.g002: Radar graphics showing the proportion of subjects and the immunological biomarkers production in different contexts.None of the individuals could be identified as high producers of cytokines in the health controls (HC) group. The infective endocarditis (IE) group presented high producers of all cytokines. The non-IE infections group presented high producers of IL-6 and IL-8.
Mentions: Another way of presenting the results of the serum concentrations of the cytokines is to point out the high producers of these immunological biomarkers in the three study groups. These results are shown in Fig 2. These data complete and illustrate the information given in Fig 1. The IE group included high producers of all cytokines analyzed. The control group of patients with infections other than IE showed an elevated proportion of high producers of IL-6 and IL-8 and a moderate prevalence of high producers of IL-10. When the IE group and the group of other infections were compared, a more elevated proportion of high producers of IL-1β, TNF-α and IL-12 was observed in the IE group. There were high producers only of IL-10 in the group of healthy controls.

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus