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Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus

Serum concentrations of inflammatory cytokines in the study groups.The groups evaluated were: health controls (HC) (n = 34, white circle), non-infective endocarditis (non-IE) infections (n = 30, light gray circle), and IE (n = 81, dark gray circle). The results were expressed as pg/mL and a line represented a median value. Significant differences at p<0.05 are highlighted by letters for difference of a group as compared to health controls indicated by ‘‘a” and to non-infective endocarditis indicated by ‘‘b”.
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pone.0133631.g001: Serum concentrations of inflammatory cytokines in the study groups.The groups evaluated were: health controls (HC) (n = 34, white circle), non-infective endocarditis (non-IE) infections (n = 30, light gray circle), and IE (n = 81, dark gray circle). The results were expressed as pg/mL and a line represented a median value. Significant differences at p<0.05 are highlighted by letters for difference of a group as compared to health controls indicated by ‘‘a” and to non-infective endocarditis indicated by ‘‘b”.

Mentions: The results of the serum concentrations of the cytokines (IL- 1β, IL-6, IL-8, IL-10, IL-12 and TNF-α) from the patients with IE and from the controls are presented in Fig 1. Except for IL-12, the patients with IE had significantly higher serum concentrations of the cytokines than the healthy donors. Median IL-1β, TNF-α and IL-12 serum levels were significantly higher in the IE patients than in the patients with non-IE infections. When the median serum concentrations of the cytokines were compared between the two control groups, a significant statistical difference was found only for IL-6 and IL-8, which were higher in the group of patients with non-IE infections.


Cytokine Signature in Infective Endocarditis.

Araújo IR, Ferrari TC, Teixeira-Carvalho A, Campi-Azevedo AC, Rodrigues LV, Guimarães Júnior MH, Barros TL, Gelape CL, Sousa GR, Nunes MC - PLoS ONE (2015)

Serum concentrations of inflammatory cytokines in the study groups.The groups evaluated were: health controls (HC) (n = 34, white circle), non-infective endocarditis (non-IE) infections (n = 30, light gray circle), and IE (n = 81, dark gray circle). The results were expressed as pg/mL and a line represented a median value. Significant differences at p<0.05 are highlighted by letters for difference of a group as compared to health controls indicated by ‘‘a” and to non-infective endocarditis indicated by ‘‘b”.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520569&req=5

pone.0133631.g001: Serum concentrations of inflammatory cytokines in the study groups.The groups evaluated were: health controls (HC) (n = 34, white circle), non-infective endocarditis (non-IE) infections (n = 30, light gray circle), and IE (n = 81, dark gray circle). The results were expressed as pg/mL and a line represented a median value. Significant differences at p<0.05 are highlighted by letters for difference of a group as compared to health controls indicated by ‘‘a” and to non-infective endocarditis indicated by ‘‘b”.
Mentions: The results of the serum concentrations of the cytokines (IL- 1β, IL-6, IL-8, IL-10, IL-12 and TNF-α) from the patients with IE and from the controls are presented in Fig 1. Except for IL-12, the patients with IE had significantly higher serum concentrations of the cytokines than the healthy donors. Median IL-1β, TNF-α and IL-12 serum levels were significantly higher in the IE patients than in the patients with non-IE infections. When the median serum concentrations of the cytokines were compared between the two control groups, a significant statistical difference was found only for IL-6 and IL-8, which were higher in the group of patients with non-IE infections.

Bottom Line: Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender.The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals.A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Infectologia e Medicina Tropical, Departamento de Clínica Médica, Faculdade de Medicina da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Infective endocarditis (IE) is a severe disease with high mortality rate. Cytokines participate in its pathogenesis and may contribute to early diagnosis improving the outcome. This study aimed to evaluate the cytokine profile in IE. Serum concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured by cytometric bead array (CBA) at diagnosis in 81 IE patients, and compared with 34 healthy subjects and 30 patients with non-IE infections, matched to the IE patients by age and gender. Mean age of the IE patients was 47±17 years (range, 15-80 years), and 40 (50%) were male. The IE patients had significantly higher serum concentrations of IL-1β, IL-6, IL-8, IL-10 and TNF-α as compared to the healthy individuals. The median levels of IL-1β, TNF-α and IL-12 were higher in the IE than in the non-IE infections group. TNF-α and IL-12 levels were higher in staphylococcal IE than in the non-staphylococcal IE subgroup. There was a higher proportion of both low IL-10 producers and high producers of IL-1β, TNF-α and IL-12 in the staphylococcal IE than in the non-staphylococcal IE subgroup. This study reinforces a relationship between the expression of proinflammatory cytokines, especially IL-1β, IL-12 and TNF-α, and the pathogenesis of IE. A lower production of IL-10 and impairment in cytokine network may reflect the severity of IE and may be useful for risk stratification.

No MeSH data available.


Related in: MedlinePlus