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The Effects of Royal Jelly on Fitness Traits and Gene Expression in Drosophila melanogaster.

Shorter JR, Geisz M, Özsoy E, Magwire MM, Carbone MA, Mackay TF - PLoS ONE (2015)

Bottom Line: We found that lower concentrations of RJ do induce significant differences in body size in both sexes; higher concentrations reduce size, increase mortality, shorten lifespan and reduce productivity.Increased concentrations of RJ also consistently lengthened development time in both sexes.The transcriptional changes are consistent with alterations in cellular processes to cope with excess nutrients provided by RJ, including biosynthesis and detoxification, which might contribute to accelerated senescence and reduced lifespan.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, United States of America; W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, North Carolina, United States of America; Program in Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Royal Jelly (RJ) is a product made by honey bee workers and is required for queen differentiation and accompanying changes in queen body size, development time, lifespan and reproductive output relative to workers. Previous studies have reported similar changes in Drosophila melanogaster in response to RJ. Here, we quantified viability, development time, body size, productivity, lifespan and genome wide transcript abundance of D. melanogaster reared on standard culture medium supplemented with increasing concentrations of RJ. We found that lower concentrations of RJ do induce significant differences in body size in both sexes; higher concentrations reduce size, increase mortality, shorten lifespan and reduce productivity. Increased concentrations of RJ also consistently lengthened development time in both sexes. RJ is associated with changes in expression of 1,581 probe sets assessed using Affymetrix Drosophila 2.0 microarrays, which were enriched for genes associated with metabolism and amino acid degradation. The transcriptional changes are consistent with alterations in cellular processes to cope with excess nutrients provided by RJ, including biosynthesis and detoxification, which might contribute to accelerated senescence and reduced lifespan.

No MeSH data available.


Genetic network of RJ differentially expressed genes.Colored boxes denote differentially expressed genes. Red, glutathione metabolism; pink, purine metabolism; green, drug metabolism and other enzymes; teal, cysteine and methionine metabolism; yellow, lysine degradation; purple, beta-alanine metabolism.
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pone.0134612.g003: Genetic network of RJ differentially expressed genes.Colored boxes denote differentially expressed genes. Red, glutathione metabolism; pink, purine metabolism; green, drug metabolism and other enzymes; teal, cysteine and methionine metabolism; yellow, lysine degradation; purple, beta-alanine metabolism.

Mentions: We used network enrichment analysis to place the differentially expressed genes significant for the treatment effect in context. We identified a genetic network of 57 genes (P < 0.01) based on global metabolic gene data [42] (Fig 3). This network is enriched for genes encoding glutathione S transferases, which detoxify xenobiotic compounds, and for genes involved in amino acid metabolism, consistent with the high levels of protein found in RJ.


The Effects of Royal Jelly on Fitness Traits and Gene Expression in Drosophila melanogaster.

Shorter JR, Geisz M, Özsoy E, Magwire MM, Carbone MA, Mackay TF - PLoS ONE (2015)

Genetic network of RJ differentially expressed genes.Colored boxes denote differentially expressed genes. Red, glutathione metabolism; pink, purine metabolism; green, drug metabolism and other enzymes; teal, cysteine and methionine metabolism; yellow, lysine degradation; purple, beta-alanine metabolism.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520568&req=5

pone.0134612.g003: Genetic network of RJ differentially expressed genes.Colored boxes denote differentially expressed genes. Red, glutathione metabolism; pink, purine metabolism; green, drug metabolism and other enzymes; teal, cysteine and methionine metabolism; yellow, lysine degradation; purple, beta-alanine metabolism.
Mentions: We used network enrichment analysis to place the differentially expressed genes significant for the treatment effect in context. We identified a genetic network of 57 genes (P < 0.01) based on global metabolic gene data [42] (Fig 3). This network is enriched for genes encoding glutathione S transferases, which detoxify xenobiotic compounds, and for genes involved in amino acid metabolism, consistent with the high levels of protein found in RJ.

Bottom Line: We found that lower concentrations of RJ do induce significant differences in body size in both sexes; higher concentrations reduce size, increase mortality, shorten lifespan and reduce productivity.Increased concentrations of RJ also consistently lengthened development time in both sexes.The transcriptional changes are consistent with alterations in cellular processes to cope with excess nutrients provided by RJ, including biosynthesis and detoxification, which might contribute to accelerated senescence and reduced lifespan.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, United States of America; W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, North Carolina, United States of America; Program in Genetics, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Royal Jelly (RJ) is a product made by honey bee workers and is required for queen differentiation and accompanying changes in queen body size, development time, lifespan and reproductive output relative to workers. Previous studies have reported similar changes in Drosophila melanogaster in response to RJ. Here, we quantified viability, development time, body size, productivity, lifespan and genome wide transcript abundance of D. melanogaster reared on standard culture medium supplemented with increasing concentrations of RJ. We found that lower concentrations of RJ do induce significant differences in body size in both sexes; higher concentrations reduce size, increase mortality, shorten lifespan and reduce productivity. Increased concentrations of RJ also consistently lengthened development time in both sexes. RJ is associated with changes in expression of 1,581 probe sets assessed using Affymetrix Drosophila 2.0 microarrays, which were enriched for genes associated with metabolism and amino acid degradation. The transcriptional changes are consistent with alterations in cellular processes to cope with excess nutrients provided by RJ, including biosynthesis and detoxification, which might contribute to accelerated senescence and reduced lifespan.

No MeSH data available.