Limits...
Whole-Retina Reduced Electrophysiological Activity in Mice Bearing Retina-Specific Deletion of Vesicular Acetylcholine Transporter.

Bedore J, Martyn AC, Li AK, Dolinar EA, McDonald IS, Coupland SG, Prado VF, Prado MA, Hill KA - PLoS ONE (2015)

Bottom Line: One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits.Reduced a-wave amplitude was proportional to the reduction in b-wave amplitude and not associated with altered a-wave 10%-90% rise time or inner and outer segment thicknesses.Reduced amplitude across the electroretinogram wave form does not suggest dysfunction in specific retinal cell types and could reflect underlying changes in the retinal and/or extraretinal microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.

ABSTRACT

Background: Despite rigorous characterization of the role of acetylcholine in retinal development, long-term effects of its absence as a neurotransmitter are unknown. One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits. The current study investigates the effects of disrupting cholinergic signalling in mice, through deletion of vesicular acetylcholine transporter (VAChT) in the developing retina, pigmented epithelium, optic nerve and optic stalk, on electrophysiology and structure of the mature retina.

Methods & results: A combination of electroretinography, optical coherence tomography imaging and histological evaluation assessed retinal integrity in mice bearing retina- targeted (embryonic day 12.5) deletion of VAChT (VAChTSix3-Cre-flox/flox) and littermate controls at 5 and 12 months of age. VAChTSix3-Cre-flox/flox mice did not show any gross changes in nuclear layer cellularity or synaptic layer thickness. However, VAChTSix3-Cre-flox/flox mice showed reduced electrophysiological response of the retina to light stimulus under scotopic conditions at 5 and 12 months of age, including reduced a-wave, b-wave, and oscillatory potential (OP) amplitudes and decreased OP peak power and total energy. Reduced a-wave amplitude was proportional to the reduction in b-wave amplitude and not associated with altered a-wave 10%-90% rise time or inner and outer segment thicknesses.

Significance: This study used a novel genetic model in the first examination of function and structure of the mature mouse retina with disruption of cholinergic signalling. Reduced amplitude across the electroretinogram wave form does not suggest dysfunction in specific retinal cell types and could reflect underlying changes in the retinal and/or extraretinal microenvironment. Our findings suggest that release of acetylcholine by VAChT is essential for the normal electrophysiological response of the mature mouse retina.

No MeSH data available.


Related in: MedlinePlus

Characterization of OP parameters elicited by VAChTSix3-Cre-flox/flox and control mice under scotopic conditions in response to 10 cd•s/m2 light stimulus.(A-B) VAChTSix3-Cre-flox/flox retinas show reductions in individual OP amplitudes in the time-domain representation of ERG OP parameters at (A) 5 and (B) 12 months of age. (C-D) VAChTSix3-Cre-flox/flox retinas also show reductions in OP total energy and power in the frequency-domain representation of ERG OP parameters at (C) 5 and (D) 12 months of age.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4520552&req=5

pone.0133989.g006: Characterization of OP parameters elicited by VAChTSix3-Cre-flox/flox and control mice under scotopic conditions in response to 10 cd•s/m2 light stimulus.(A-B) VAChTSix3-Cre-flox/flox retinas show reductions in individual OP amplitudes in the time-domain representation of ERG OP parameters at (A) 5 and (B) 12 months of age. (C-D) VAChTSix3-Cre-flox/flox retinas also show reductions in OP total energy and power in the frequency-domain representation of ERG OP parameters at (C) 5 and (D) 12 months of age.

Mentions: VAChTSix3-Cre-flox/flox mice show changes in OP parameters that are apparent in both the time and frequency domain (Fig 6). The maximum power of the OPs (Fig 7A and 7B; P < 0.0001) as well as the total energy of the OPs (Fig 7C and 7D; P < 0.0001) are significantly lower in VAChTSix3-Cre-flox/flox than littermate control mice at both ages examined (Fig 7A and 7B; P < 0.0001). There are no changes in the dominant frequencies of the OPs between VAChTSix3-Cre-flox/flox mice and littermate controls at either age (Fig 7E and 7F).


Whole-Retina Reduced Electrophysiological Activity in Mice Bearing Retina-Specific Deletion of Vesicular Acetylcholine Transporter.

Bedore J, Martyn AC, Li AK, Dolinar EA, McDonald IS, Coupland SG, Prado VF, Prado MA, Hill KA - PLoS ONE (2015)

Characterization of OP parameters elicited by VAChTSix3-Cre-flox/flox and control mice under scotopic conditions in response to 10 cd•s/m2 light stimulus.(A-B) VAChTSix3-Cre-flox/flox retinas show reductions in individual OP amplitudes in the time-domain representation of ERG OP parameters at (A) 5 and (B) 12 months of age. (C-D) VAChTSix3-Cre-flox/flox retinas also show reductions in OP total energy and power in the frequency-domain representation of ERG OP parameters at (C) 5 and (D) 12 months of age.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4520552&req=5

pone.0133989.g006: Characterization of OP parameters elicited by VAChTSix3-Cre-flox/flox and control mice under scotopic conditions in response to 10 cd•s/m2 light stimulus.(A-B) VAChTSix3-Cre-flox/flox retinas show reductions in individual OP amplitudes in the time-domain representation of ERG OP parameters at (A) 5 and (B) 12 months of age. (C-D) VAChTSix3-Cre-flox/flox retinas also show reductions in OP total energy and power in the frequency-domain representation of ERG OP parameters at (C) 5 and (D) 12 months of age.
Mentions: VAChTSix3-Cre-flox/flox mice show changes in OP parameters that are apparent in both the time and frequency domain (Fig 6). The maximum power of the OPs (Fig 7A and 7B; P < 0.0001) as well as the total energy of the OPs (Fig 7C and 7D; P < 0.0001) are significantly lower in VAChTSix3-Cre-flox/flox than littermate control mice at both ages examined (Fig 7A and 7B; P < 0.0001). There are no changes in the dominant frequencies of the OPs between VAChTSix3-Cre-flox/flox mice and littermate controls at either age (Fig 7E and 7F).

Bottom Line: One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits.Reduced a-wave amplitude was proportional to the reduction in b-wave amplitude and not associated with altered a-wave 10%-90% rise time or inner and outer segment thicknesses.Reduced amplitude across the electroretinogram wave form does not suggest dysfunction in specific retinal cell types and could reflect underlying changes in the retinal and/or extraretinal microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.

ABSTRACT

Background: Despite rigorous characterization of the role of acetylcholine in retinal development, long-term effects of its absence as a neurotransmitter are unknown. One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits. The current study investigates the effects of disrupting cholinergic signalling in mice, through deletion of vesicular acetylcholine transporter (VAChT) in the developing retina, pigmented epithelium, optic nerve and optic stalk, on electrophysiology and structure of the mature retina.

Methods & results: A combination of electroretinography, optical coherence tomography imaging and histological evaluation assessed retinal integrity in mice bearing retina- targeted (embryonic day 12.5) deletion of VAChT (VAChTSix3-Cre-flox/flox) and littermate controls at 5 and 12 months of age. VAChTSix3-Cre-flox/flox mice did not show any gross changes in nuclear layer cellularity or synaptic layer thickness. However, VAChTSix3-Cre-flox/flox mice showed reduced electrophysiological response of the retina to light stimulus under scotopic conditions at 5 and 12 months of age, including reduced a-wave, b-wave, and oscillatory potential (OP) amplitudes and decreased OP peak power and total energy. Reduced a-wave amplitude was proportional to the reduction in b-wave amplitude and not associated with altered a-wave 10%-90% rise time or inner and outer segment thicknesses.

Significance: This study used a novel genetic model in the first examination of function and structure of the mature mouse retina with disruption of cholinergic signalling. Reduced amplitude across the electroretinogram wave form does not suggest dysfunction in specific retinal cell types and could reflect underlying changes in the retinal and/or extraretinal microenvironment. Our findings suggest that release of acetylcholine by VAChT is essential for the normal electrophysiological response of the mature mouse retina.

No MeSH data available.


Related in: MedlinePlus